95 research outputs found
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Cytokine polymorphism differences demonstrated between living related HLA identical donor/ recipient pairs
Evaluation of the tolerogenic effects of donor bone marrow cells using a severe combined immunodeficient mouse–human islet transplant model
The immunoregulatory role of human donor bone marrow cells (DBMC) has been studied extensively in our laboratory using
in vitro and
ex vivo assays. However, new experimental systems that can overcome the limitations of tissue culture assays but with more clinical relevance than purely animal experimentation, needed to be generated. Therefore we have developed a new human peripheral blood lymphocyte (PBL) severe combined immunodeficient (SCID) mouse islet transplantation model without the occurrence of graft-versus-host disease (GvHD) and have used it to evaluate the tolerogenic effects of DBMC. Nonobese diabetogenic (NOD)–SCID mice were transplanted with human deceased donor islets and were reconstituted with human PBL (allogeneic to islets; denoted as recipient) with or without DBMC from the islet donor.
It was observed that the most cellularly economical dose was 3000 islets per animal and that injection into the portal vein was better than implantation under the kidney capsule. Even though maximal lymphoid reconstitution was observed with 40-million fresh and anti-CD3 activated recipient PBL (conventional method), the mice developed severe graft GvHD. However, with the new method of reconstitution where animals were injected with 20-million anti-CD3-activated plus 40-million anti–donor-activated recipient PBL, no discernible GvHD was observed. More importantly, this latter method was associated with islet transplant rejection, which in turn could be abrogated by co-injection of the animals with DBMC. These in vivo results confirmed our previous in vitro observations that human DBMC have regulatory activity
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IN VIVO AND IN VITRO INDUCTION OF CLASS II MOLECULES ON CANINE RENAL CELLS AND THEIR EFFECT ON THE MIXED LYMPHOCYTE KIDNEY CELL CULTURE
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Induction of T regulatory cells by stimulation with immature autologous dendritic cell enriched population
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POSTTRANSPLANT HYPERGLYCEMIA Increased Incidence in Cyclosporine-Treated Renal Allograft Recipients
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DETECTION OF ANTIBODY TO HEPATITIS C VIRUS IN RENAL TRANSPLANT RECIPIENTS
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Renal Transplantation in Systemic Lupus erythematosus: One Center’s Experience
A retrospective analysis of 15 renal transplant patients with end-stage renal disease (ESRD) secondary to systemic lupus erythematosus (SLE) was performed. Overall actuarial patient and graft survival at 6 years was 93 and 84%, respectively. Recipients of HLA-identical kidneys did not appear to be at increased risk of allograft failure due to rejection or recurrent disease. Two biopsy-proven cases of recurrent lupus involving the allograft were observed and are discussed. Those patients currently experiencing excellent graft function (creatinine < 2 mg/dl) had a significantly longer pretransplantation dialytic interval than the group whose most recent serum creatinine exceeds 2 mg/dl (or returned to dialysis). Posttransplantation monitoring of antinuclear antibody, antidouble-stranded DNA, C3, C4, and circulating immune complexes was not predictive of renal or extrarenal disease activity. Renal transplantation should be considered an excellent therapeutic modality for the lupus patient with ESRD, although an interim period on dialysis of at least 1 year seems warranted
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THE BIOLOGIC SIGNIFICANCE OF THE MIXED LYMPHOCYTE KIDNEY CULTURE IN HUMANS
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