2 research outputs found

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    Not AvailableA new leaf blight disease of browntop millet (Brachiaria ramosa) was noticed during rainy season (Kharif) 2018 at small millet experimental field, University of Agricultural Sciences, Gandhi Krishi Vignana Kendra (GKVK), Bengaluru, India. To assess the disease severity, an intensive roving survey was conducted during the 2019 cropping season. Based on the morphological characterization, the causal agent of leaf blight disease was identified as Bipolaris spp. Further sequencing and combined gene analysis of ITS (internal transcribed spacer of rDNA), GAPDH (glyceraldehyde 3-phosphate dehydrogenase) and LSU (large subunit) of all the nine isolates confirmed the pathogen as B. setariae. Pathogenicity study showed that all the isolates were pathogenic and caused leaf blight symptoms on browntop millet. The B. setariae isolates showed marked variability with respect to disease incidence on browntop millet (cv. Dundu korale) under artificial inoculation conditions. However, the host range was limited only to browntop millet and found non-pathogenic to other six small millets examined. To our knowledge, this is the first completely described study on characterization of B. setariae causing leaf blight disease of browntop millet in India.Not Availabl

    Phenotypic and Genotypic screening of fifty-two rice (Oryza sativa L.) genotypes for desirable cultivars against blast disease.

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    Magnaporthe oryzae, the rice blast fungus, is one of the most dangerous rice pathogens, causing considerable crop losses around the world. In order to explore the rice blast-resistant sources, initially performed a large-scale screening of 277 rice accessions. In parallel with field evaluations, fifty-two rice accessions were genotyped for 25 major blast resistance genes utilizing functional/gene-based markers based on their reactivity against rice blast disease. According to the phenotypic examination, 29 (58%) and 22 (42%) entries were found to be highly resistant, 18 (36%) and 29 (57%) showed moderate resistance, and 05 (6%) and 01 (1%), respectively, were highly susceptible to leaf and neck blast. The genetic frequency of 25 major blast resistance genes ranged from 32 to 60%, with two genotypes having a maximum of 16 R-genes each. The 52 rice accessions were divided into two groups based on cluster and population structure analysis. The highly resistant and moderately resistant accessions are divided into different groups using the principal coordinate analysis. According to the analysis of molecular variance, the maximum diversity was found within the population, while the minimum diversity was found between the populations. Two markers (RM5647 and K39512), which correspond to the blast-resistant genes Pi36 and Pik, respectively, showed a significant association to the neck blast disease, whereas three markers (Pi2-i, Pita3, and k2167), which correspond to the blast-resistant genes Pi2, Pita/Pita2, and Pikm, respectively, showed a significant association to the leaf blast disease. The associated R-genes might be utilized in rice breeding programmes through marker-assisted breeding, and the identified resistant rice accessions could be used as prospective donors for the production of new resistant varieties in India and around the world
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