Perfusion Decellularization of Extrahepatic Bile Duct Allows Tissue-Engineered Scaffold Generation by Preserving Matrix Architecture and Cytocompatibility

Reconstruction of bile ducts damaged remains a vexing medical problem. Surgeons have few options when it comes to a long segment reconstruction of the bile duct. Biological scaffolds of decellularized biliary origin may offer an approach to support the replace of bile ducts. Our objective was to obtain an extracellular matrix scaffold derived from porcine extrahepatic bile ducts (dECM-BD) and to analyze its biological and biochemical properties. The efficiency of the tailored perfusion decellularization process was assessed through histology stainings. Results from 4’-6-diamidino-2-phenylindole (DAPI), Hematoxylin and Eosin (H&E) stainings, and deoxyribonucleic acid (DNA) quantification showed proper extracellular matrix (ECM) decellularization with an effectiveness of 98%. Immunohistochemistry results indicate an effective decrease in immunogenic marker as human leukocyte antigens (HLA-A) and Cytokeratin 7 (CK7) proteins. The ECM of the bile duct was preserved according to Masson and Herovici stainings. Data derived from scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) showed the preservation of the dECM-BD hierarchical structures. Cytotoxicity of dECM-BD was null, with cells able to infiltrate the scaffold. In this work, we standardized a decellularization method that allows one to obtain a natural bile duct scaffold with hierarchical ultrastructure preservation and adequate cytocompatibility

Veterinaria - Mexico 26 3 277 282

The spleen is the most common primary site for hemangiosarcoma in dogs. The tumor is characterized by rapid growth and widespread metastasis, presumably due to both its tissue of origin, the vascular endothelium, and its resultant ready access to systemic circulation. The most common clinical presentation is a palpable abdominal mass, hemoperitoneum, anemia, shock and disseminated intravascular coagulopathy. At the Small Annual Species Hospital (National Autonomous University of Mexico) arrived a German Shepherd dog, a 7 year old male with the next signs: Abdominal distention, depression and anorexia. At physical examination it showed weak pulse, cyanosis, high respiratory frequency and liquid in the abdominal cavity. The radiological study showed an image that was compatible with a peritoneal effusion. An abdominal puncture was made, and a sanguinolent liquid was obtained. Fluid therapy and blood transfusion were renovated and the hematocrit was decreased. After the therapy were established, a celiotomy extracting near 41 of intraperitoneal hemorrhagic liquid was done. Multiple tumoral nodules were observed in the spleen showing the origin of the bleeding. The liver presented several metastatic foci. A total splenectomy was performed taking also a biopsy of the hepatic nodules. The necropsy revealed large areas of hemorrhages and subcutaneous edema in the ventral abdomen. The liver had numerous subscapular hemorrhagic nodules; the other abdominal viscerae were icteric, and a few petechial hemorrhages were found on the serosae. Microscopic study of the affected organs showed primary foci of hemangiosarcoma in the spleen and metastasis to liver, epiploa and bronchial lymph nodules. There was also disseminated intravascular coagulation, because there was the presence of fibrin thrombi in alveolar capillaries in lungs and in glomerular capillaries in kidneys

Pyothorax by linear foreign body

Se reporta el caso de un gato europeo doméstico de un año y tres meses de edad presentado a consulta con patrón respiratorio restrictivo, dolor a la palpación abdominal en la región cráneo ventral, sonidos cardiacos disminuidos. Se realizó estudio radiográfico rutinario de tórax que permitió observar un cuerpo extraño lineal (CEL) radio-opaco con pérdida de definición de las estructuras y signos de derrame pleural. Se practicó toracotomía exploratoria en hemitórax izquierdo para extraer el CEL y contenido purulento. Asimismo, se colocó drenaje pleural. En el postoperatorio inmediato se administró oxigenoterapia y permaneció en hospitalización durante una semana. Al mes se realizaron estudios radiográficos de control donde no se observaron cambios aparentes

Pyothorax by linear foreign body

Se reporta el caso de un gato europeo doméstico de un año y tres meses de edad presentado a consulta con patrón respiratorio restrictivo, dolor a la palpación abdominal en la región cráneo ventral, sonidos cardiacos disminuidos. Se realizó estudio radiográfico rutinario de tórax que permitió observar un cuerpo extraño lineal (CEL) radio-opaco con pérdida de definición de las estructuras y signos de derrame pleural. Se practicó toracotomía exploratoria en hemitórax izquierdo para extraer el CEL y contenido purulento. Asimismo, se colocó drenaje pleural. En el postoperatorio inmediato se administró oxigenoterapia y permaneció en hospitalización durante una semana. Al mes se realizaron estudios radiográficos de control donde no se observaron cambios aparentes