The Nascent Kuroshio of Lamon Bay

A northward flowing current, emanating from the North Equatorial Current (NEC) bifurcation at the Philippine margin, enters Lamon Bay along Luzon's eastern coast. There the NEC tropical water masses merge with subtropical water of the western North Pacific to form the Kuroshio. A northward flowing western boundary current is first observed near 16.5°N, marking the initiation of the Kuroshio. The current feeding into the nascent Kuroshio of Lamon Bay is bracketed by an anticyclonic dipole to its northeast and a cyclonic dipole to its southwest. Ship based observational programs in the spring seasons of 2011 and 2012, detect a shift of the Lamon Bay thermohaline stratification with marked enrichment of NEC tropical thermocline water in 2012 relative to a dominant western North Pacific subtropical stratification of 2011. Temperature - salinity time series from moorings spanning the two ship based observations, identify the timing of the transition as December 2011. The NEC bifurcation was further south in May 2012 than in May 2011. We suggest that the more southern bifurcation in May 2012 induced increased NEC thermocline water injection into Lamon Bay and nascent Kuroshio, increasing the linkage of the western North Pacific subtropical and tropical thermoclines. This connection was reduced in May 2011 as the NEC bifurcation shifted into a more northerly position and western North Pacific subtropical thermocline dominated Lamon Bay stratification

Dual overflows into the deep Sulu Sea

The Sulu Sea, isolated from the neighboring ocean below 570 m, is nearly isothermal below 1250 m but with a marked salinity increase with depth. The source of the deep Sulu Sea water has been attributed to South China Sea water overflowing the 570 m topographic sill of Panay Strait. However, the Panay overflow (estimated as 0.32 × 106 m3/sec) is an unlikely source for the saltier water Sulu Sea deep water. We propose that deep Sulu Sea ventilation is derived from the south, from the Sulawesi Sea through Sibutu Passage. Sulawesi Sea water between 245 to 527 m, is mixed and heaved over the Sibutu Passage 234 m sill by the energetic tidal environment. Oxygen concentrations within the deep Sulu Sea suggest that the Sulawesi overflow is 0.15 × 106 m3/sec, with a residence time of Sulu Sea deep water of 60 years. The deep tropical Sulu Sea has the unique distinction of being ventilated from two separate sources, whose ratio may fluctuate across a range of temporal scales, associated with regional thermocline depth changes

The influence of the Bass Strait outflow on the Tasman Sea central water

An advective box—model based on mass and salt conservation arguments and volume of Tasman Sea Central Water for different temperatures and salinities has been used to determine the influence of the seasonal outflow from Bass Strait on the Tasman Sea thermocline as well as to identify the regions where previously observed strong positive salinity anomalies occur. Of the total salt flux into the layer bounded by the 25.73 and 26.96 at isopycnal surface s, about 8% is supplied by the prescribed Bass Strait transport of 0.45 Sv while 34% is accounted for by downwelling from the overlying high salinity water. The highest vertical salt flux occurs in the Southern Tasman Sea somewhere between 37° and 43° S. This corresponds to the region where positive salinity anomalies were found to occur, characterized by large salinity gradients and the existence of offshore salinity maxima along isothermal and isopycnal surfaces. The distribution of the Bass Strait Water volume in the Tasman Sea displays a similar pattern suggesting that the seasonal intrusion of BSW may act as a trigger to entrain high salinity water from the upper layers by double diffusive convection. Double —diffusive arguments are also presented to explain some of the features of the intrusion of Bass Strait Water in the Tasman Sea

Movement of Water Across Passages Connecting Philippine Inland Sea Basins

Advection of Pacific water to the inland seas is through a number of straits bordering the archipelago. Movement of water was demonstrated by temperature-salinity diagrams plotted for a number of stations situated along the various passages. As water from the Pacific flowed through the straits its characteristic T-S profile was modified as it mixed with waters of different properties. This was best seen along the San Bernardino-Verde Island transect where strong surface flow during the NE monsoon resulted in separation of profiles at the surface indicating dilution as water moved away from the source. For deeper water, the erosion of the subsurface salinity minimum and maximum representing the core of the intermediate waters showed transport. These waters were restricted by shallow sill along the eastern coast of the country and limited to a depth of 441m by the sill across the Mindoro Strait

Barrier Layer Control of Entrainment and Upwelling in the Bohol Sea, Philippines

The Bohol Sea is a relatively unstudied marginal sea located in the southern part of the Philippines. Hydrographic data from the Philippines Experiment (PhilEx) cruises reveal a complex three-dimensional circulation composed of two overturning cells that may be referred to as “double-estuarine type.” This type of overturning circulation promotes upwelling of cold, nutrient-rich waters within the Bohol Sea associated with entrainment and eddy processes that promote phytoplankton blooms. Evidence from ocean color imagery supports entrainment in the eastern basin and eddy formation in the southwestern basin. However, PhilEx researchers found that the cyclonic Iligan Bay Eddy in the southwestern basin did not conform to the paradigm of cyclonic eddy upwelling. Although upwelling was evident through doming isotherms within the water column, the surface projection of this signal was suppressed by the presence of a thick barrier layer, particularly during cruises in December 2007 and January 2008, a known La Niña period. Long-term trends in chlorophyll data followed trends in rainfall and the ENSO 3.4 index, with elevated (reduced) chlorophyll during dry El Niño years (wet La Niña years). By promoting stability of surface layer stratification and preventing vertical transport of nutrients, the barrier layer is thus a mechanism by which the El Niño-Southern Oscillation influences phytoplankton biomass in the Bohol Sea

Population genetic structure of the sea urchin Tripneustes gratilla from selected sites in Western Luzon and Eastern Philippines

The genetic structure of the sea urchin Tripneustes gratilla was investigated for six wild populations in western Luzon and eastern Philippines and one batch of hatchery-reared sea urchins. Analysis of six polymorphic allozyme loci revealed low levels of genetic differentiation (Nei’s unbiased D = 0.0 – 0.014, overall FST =0.0122), implying extensive gene flow between populations. The batch of cultured T. gratilla showed no apparent genetic distinctness from wild populations. Genetic differentiation was slightly higher in one eastern population (Ticao, Masbate). Structuring was not associated with geographic distance, but might be a result of heterogeneous recruitment. Initial results suggest that intensively-fished T. gratilla populations in northwestern Luzon should be managed as a single unit, hence a region-wide fishery management scheme needs to be implemented. The establishment of a network of marine protected areas along the western coast of Luzon is also recommende

Predicting reef fish connectivity from biogeographic patterns and larval dispersal modelling to inform the development of marine reserve networks

Developing networks of no-take marine reserves is often hindered by uncertainty in the extent to which local marine populations are connected to one another through larval dispersal and recruitment (connectivity). While patterns of connectivity can be predicted by larval dispersal models and validated by empirical methods, biogeographic approaches have rarely been used to investigate connectivity at spatial scales relevant to reserve networks (10's-100's of km). Here, species assemblage patterns in coral reef fish were used together with an individual-based model of dispersal of reef fish larvae to infer patterns of connectivity in a similar to 300 km wide region in the Philippines that included the Bohol Sea and adjacent bodies of water. A dominant current flows through the study region, which may facilitate connectivity among >100 no-take reserves. Connectivity was first investigated by analysing data on the presence/absence of 216 species of reef fish and habitat variables across 61 sites. Hierarchical clustering of sites reflecting species assemblage patterns distinguished a major group of sites in the Bohol Sea (Bray-Curtis similarity >70%) from sites situated in adjacent bodies of water (bays, channels between islands and a local sea). The grouping of sites could be partly explained by a combination of degree of embayment, % cover of sand and % cover of rubble (Spearman rank correlation, rho(w) = 0.42). The individual-based model simulated dispersal of reef fish larvae monthly for three consecutive years in the region. The results of simulations, using a range of pelagic larval durations (15-45 days), were consistent with the species assemblage patterns. Sites in the model that showed strongest potential connectivity corresponded to the majority of sites that comprised the Bohol Sea group suggested by hierarchical clustering. Most sites in the model that exhibited weak connectivity were groups of sites which had fish assemblages that were least similar to those in the Bohol Sea group. Concurrent findings from the two approaches suggest a strong influence of local oceanography and geography on broad spatial patterns of connectivity. The predictions can be used as an initial basis to organise existing reserves to form ecologically meaningful networks. This study showed that species assemblage patterns could be a viable supplementary indicator of connectivity if used together with predictions from a larval dispersal model and if the potential effect of habitat on the structuring of species assemblages is taken into consideration

Predicting reef fish connectivity from biogeographic patterns and larval dispersal modelling to inform the development of marine reserve networks

Developing networks of no-take marine reserves is often hindered by uncertainty in the extent to which local marine populations are connected to one another through larval dispersal and recruitment (connectivity). While patterns of connectivity can be predicted by larval dispersal models and validated by empirical methods, biogeographic approaches have rarely been used to investigate connectivity at spatial scales relevant to reserve networks (10's–100's of km). Here, species assemblage patterns in coral reef fish were used together with an individual-based model of dispersal of reef fish larvae to infer patterns of connectivity in a ∼300 km wide region in the Philippines that included the Bohol Sea and adjacent bodies of water. A dominant current flows through the study region, which may facilitate connectivity among >100 no-take reserves. Connectivity was first investigated by analysing data on the presence/absence of 216 species of reef fish and habitat variables across 61 sites. Hierarchical clustering of sites reflecting species assemblage patterns distinguished a major group of sites in the Bohol Sea (Bray–Curtis similarity >70%) from sites situated in adjacent bodies of water (bays, channels between islands and a local sea). The grouping of sites could be partly explained by a combination of degree of embayment, % cover of sand and % cover of rubble (Spearman rank correlation, ρw = 0.42). The individual-based model simulated dispersal of reef fish larvae monthly for three consecutive years in the region. The results of simulations, using a range of pelagic larval durations (15–45 days), were consistent with the species assemblage patterns. Sites in the model that showed strongest potential connectivity corresponded to the majority of sites that comprised the Bohol Sea group suggested by hierarchical clustering. Most sites in the model that exhibited weak connectivity were groups of sites which had fish assemblages that were least similar to those in the Bohol Sea group. Concurrent findings from the two approaches suggest a strong influence of local oceanography and geography on broad spatial patterns of connectivity. The predictions can be used as an initial basis to organise existing reserves to form ecologically meaningful networks. This study showed that species assemblage patterns could be a viable supplementary indicator of connectivity if used together with predictions from a larval dispersal model and if the potential effect of habitat on the structuring of species assemblages is taken into consideration

Nutrient load estimates for Manila Bay, Philippines using population data

A major source of nutrient load to periodically hypoxic Manila Bay is the urban nutrient waste water flow from humans and industries to surface water. In Manila alone, the population density is as high as 19,137 people/km2. A model based on a global point source model by Morée et al. (2013) was used to estimate the contribution of the population to nitrogen and phosphorus emissions which was then used in a water transport model to estimate the nitrogen (N) and phosphorus (P) loads to Manila Bay. Seven scenarios for 2050 were tested, with varying degrees and amounts for extent of sewage treatment, and population growth rates were also included. In scenario 1, the sewage connection and treatment remains the same as 2010; in scenario 2, sewage connection is improved but the treatment is the same; in scenario 3, the sewage connection as well as treatment is improved (70% tertiary); and in scenario 4, a more realistic situation of 70% primary treatment achieved with 100% connection to pipes is tested. Scenarios 5, 6, and 7 have the same parameters as 1, 2, and 3 respectively, but with the population growth rate per province reduced to half of what was used in 1, 2, and 3. In all scenarios, a significant increase in N and P loads was observed (varying from 27% to 469% relative to 2010 values). This was found even in scenario 3 where 70% of the waste water undergoes tertiary treatment which removes 80% N and 90% P. However, the lowest increase in N and P load into the bay was achieved in scenarios 5 to 7 where population growth rate is reduced to half of 2010 values. The results suggest that aside from improving sewage treatment, the continued increase of the human population in Manila at current growth rates will be an important determinant of N and P load into Manila Bay

Diversity and community structure of marine microbes around the Benham Rise underwater plateau, northeastern Philippines

Microbes are central to the structuring and functioning of marine ecosystems. Given the remarkable diversity of the ocean microbiome, uncovering marine microbial taxa remains a fundamental challenge in microbial ecology. However, there has been little effort, thus far, to describe the diversity of marine microorganisms in the region of high marine biodiversity around the Philippines. Here, we present data on the taxonomic diversity of bacteria and archaea in Benham Rise, Philippines, Western Pacific Ocean, using 16S V4 rRNA gene sequencing. The major bacterial and archaeal phyla identified in the Benham Rise are Proteobacteria, Cyanobacteria, Actinobacteria, Bacteroidetes, Marinimicrobia, Thaumarchaeota and, Euryarchaeota. The upper mesopelagic layer exhibited greater microbial diversity and richness compared to surface waters. Vertical zonation of the microbial community is evident and may be attributed to physical stratification of the water column acting as a dispersal barrier. Canonical Correspondence Analysis (CCA) recapitulated previously known associations of taxa and physicochemical parameters in the environment, such as the association of oligotrophic clades with low nutrient surface water and deep water clades that have the capacity to oxidize ammonia or nitrite at the upper mesopelagic layer. These findings provide foundational information on the diversity of marine microbes in Philippine waters. Further studies are warranted to gain a more comprehensive picture of microbial diversity within the region