Comparative study of job satisfaction in workers with a degree in Physical Activity and Sports Science

Job satisfaction is the favourable or unfavourable subjective feeling through which workers perceive their job. Numerous studies show the significant relationship between job satisfaction, motivation, performance and the quality of services. Thereby, the success of a sports organization depends upon the internal client to a great extent. However, job satisfaction an important subjective emotional variable that has been poorly evaluated in the sports sector. The aim of this study was to compare five professional profiles with a degree in Physical Activity and Sports Science, through the Herzberg’s theory of job satisfaction. The sample consisted of 974 university graduates from the entire Spanish territory with a degree in Physical Activity and Sports Science who had a relevant position in this field (69.8% men; 30.2% women), of whom more than half were in the age groups of 26 to 30 years (33.8%) and 31 to 35 years (23.4%). Based on the findings of this study, there were differences in job satisfaction between workers of Physical Activity and Sports Science according to the professional scope (teaching, management, fitness, training/high performance and other scopes)

Numerical simulation of heat transfer in a pipe with non-homogeneous thermal boundary condition

Direct numerical simulations of heat transfer in a fully-developed turbulent pipe flow with circumferentially-varying thermal boundary conditions are reported. Three cases have been considered for friction Reynolds number in the range 180–360 and Prandtl number in the range 0.7–4. The temperature statistics under these heating conditions are characterized. Eddy diffusivities and turbulent Prandtl numbers for radial and circumferential directions are evaluated and compared to the values predicted by simple models. It is found that the usual assumptions made in these models provide reasonable predictions far from the wall and that corrections to the models are needed near the wall.O.F. and M.G.-V. were partially supported by Grant TRA2013-41103-P of the Spanish Ministry of Economy and Competitiveness. This grant includes FEDER funding

Analysis of Hop Acids and Their Oxidized Derivatives and Iso-α-acids in Beer by Capillary Electrophoresis−Electrospray Ionization Mass Spectrometry

This study investigates the applicability of on-line coupling of capillary electrophoresis with electrospray ionization tandem mass spectrometry (CZE-ESI-MS) for the separation and characterization of α ¬and β-acids and oxidized hop acids from crude extracts of different hop varieties. CZE-ESI-MS with negative-ion electrospray ionization proved to be a suitable technique for the determination of these types of natural compounds and their oxidized derivatives. The CZE parameters (pH, concentration, and buffer type) and ESI-MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure, drying gas flow rate, temperature, and compound stability) were optimized. The optimized method provides the potential for a fast qualitative determination of hop acids and their oxidation compounds. The method was also applied to the determination of iso-α-acids in beer

Una subespecie nueva en el género Sideritis L. (Lamiaceae)

.

Comparative metabolomic study of transgenic versus conventional soybean using capillary electrophoresis–time-of-flight mass spectrometry

In this work, capillary electrophoresis–time-of-flight mass spectrometry (CE–TOF-MS) is proposed to identify and quantify the main metabolites found in transgenic soybean and its corresponding non-transgenic parental line both grown under identical conditions. The procedure includes optimization of metabolites extraction, separation by CE, on-line electrospray-TOF-MS analysis and data evaluation. A large number of extraction procedures and background electrolytes are tested in order to obtain a highly reproducible and sensitive analytical methodology. Using this approach, a large number of metabolites were tentatively identified based on the high mass accuracy provided by TOF-MS analyzer, together with the isotopic pattern and expected electrophoretic mobility of these compounds. In general, the same metabolites and in similar amounts were found in the conventional and transgenic variety. However, significant differences were also observed in some specific cases when the conventional variety was compared with its corresponding transgenic line. The selection of these metabolites as possible biomarkers of transgenic soybean is discussed, although a larger number of samples need to be analyzed in order to validate this point. It is concluded that metabolomic procedures based on CE-MS can open new perspectives in the study of transgenic foods in order to corroborate (or not) the equivalence with their conventional counterparts.Peer reviewe

Nano- and rapid resolution liquid chromatography-electrospray ionization-time of flight mass spectrometry to identify and quantify phenolic compounds in olive oil

The applicability of nano-liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (nanoLC-ESI-TOF MS) for the analysis of phenolic compounds in olive oil was studied and compared with a HPLC method. After the injection, the compounds were focused on a short capillary trapping column (100 μm i.d., effective length 20 mm, 5 μm particle size) and then nanoLC analysis was carried out in a fused silica capillary column (75 μm i.d., effective length 10 cm, 3 μm particle size) packed with C18 stationary phase. The mobile phase was a mixture of water + 0.5% acetic acid and acetonitrile eluting at 300 nL/min in a gradient mode. Phenolic compounds from different families were identified and quantified. The quality parameters of the nanoLC method (linearity, limits of detection and quantification, repeatability) were evaluated and compare to those obtained with HPLC. The new methodology presents better sensitivity (reaching LOD values below 1 ppb) with less consumption of mobile phases, but worse repeatability, especially inter-day repeatability, doing more difficult to get highly accurate quantification. The results described in this paper open up the application fields of this technique to cover a larger variety of compounds and its advantages will make it especially useful for the analysis of samples containing low concentration of phenolic compounds, as for instance, in biological samples.Ministry of Education and Science (FPU, AP2005- 4356 and Proyect AGL 2008-05108-CO3-03/ALI), and Junta de Andalucía (Proyect P07-AGR-02619)

Uptake and metabolism of olive oil polyphenols in human breast cancer cells using nano-liquid chromatography coupled to electrospray ionization–time of flight-mass spectrometry

Polyphenols from extra virgin olive oil (EVOO), a main component of the Mediterranean diet, have demonstrated repeatedly anti-tumor activity in several in vitro and in vivo studies. However, little is known about the efficiency of the absorption process and metabolic conversion of these compounds at cellular level. In this study, a nano liquid chromatography–electrospray ionization–time of flight mass spectrometry (nanoLC–ESI–TOF MS) method was developed to study the cellular uptake and metabolism of olive oil phenols in JIMT-1 human breast cancer cells. After incubation for different time periods with EVOO-derived phenolic extracts, culture media, cytosolic fraction and solid particles fraction were separated and analyzed. Most of the free phenols, mainly hydroxytyrosol, its secoiridoid derivatives, and the flavonoid luteolin, disappeared in the culture media in different ways and at different times. Besides, several metabolites were detected in the culture media, fact that may indicate absorption and intracellular metabolism followed by rapid cellular export. Low intracellular accumulation was observed with only traces of some compounds detected in the cytosolic and solid particles fractions. Methylated conjugates were the major metabolites detected, suggesting a catalytic action of catechol-O-methyl transferase (COMT) in cancer cells.The authors are very grateful to Ministry of Education and Science (FPU, AP2005-4356) and Junta de Andalucia (project P09-FQM-5469, project P07-AGR-02619 and AGL 2008-05108- CO3-03/ALI). Besides, this work was supported in part by Instituto de Salud Carlos III (Ministerio de Sanidad y Consumo, Fondo de Investigación Sanitaria – FIS, Spain, Grants CP05-00090, PI06-0778 and RD06-0020-0028)

Syndrome coronarien aigu chez une jeune patiente: au-delà des lésions coronaires

Nous exposons le cas d’une patiente de 47 ans qui présente un syndrome coronarien aigu et une probable embolie artérielle du membre inférieur droit dans l’étude duquel nous détectons la présence de la mutation du gène de la prothrombine G201210A associée à la présence de facteur anticoagulant lupique. La patiente a bénéficié d’une transplantation cardiaque avec bonne évolution clinique

Exploratory analysis of human urine by LC–ESI-TOF MS after high intake of olive oil: understanding the metabolism of polyphenols

Olive oil polyphenols have important biological properties which closely depend on their bioavailability, therefore it is essential to understand how polyphenols are absorbed, metabolized and eliminated from the body. An analytical methodology based on rapid resolution liquid chromatography (RRLC) coupled to mass spectrometry detection with a time of flight analyzer (RRLC-ESI-TOF MS) was developed for the determination of the main olive oil phenolic compounds and their metabolites in human urine. Urine samples from ten healthy volunteers were collected before and 2, 4 and 6 h after the intake of 50 mL of extra-virgin olive oil. The proposed method includes liquid-liquid extraction with ethyl acetate that provides extraction recoveries of the phenolic compounds studied between 35 and 75% from spiked urine samples. Good repeatability was obtained, since the relative standard deviations (RSDs) of peak areas in the intra- and inter-day studies were 4.3 and 6.5%, respectively. Statistical studies allowed us to discriminate between the urine samples before and after the intake, and facilitated to find out the m/z values responsible of this discrimination. Based on the very accurate mass information and the isotopic pattern provided by TOF-MS analyzer, together with other available information, ten of these biomarkers and more than 50 metabolites, obtained through phase I and phase II biotransformation reactions, were tentatively identified. Additionally, kinetic studies of the metabolites identified as possible biomarkers were developed, obtaining maximal values in the first two hours for most compounds.The authors are very grateful to Ministry of Education and Science (FPU, AP2005-4356 and Project AGL 2008- 05108-613 CO3-03/ALI), and Junta de Andalucía (P07-AGR-02629 and P09-FQM-5469)