Synthesis and characterization of novel 2, 2'-bipyrimidine fluorescent derivative for protein binding

<p>Abstract</p> <p>Background</p> <p>Fluorescent dyes with biocompatible functional group and good fluorescence behavior are used as biosensor for monitoring different biological processes as well as detection of protein assay. All reported fluorophore used as sensors are having high selectivity and sensitivity but till there is more demand to synthesized new fluorophore which have improved fluorescence properties and good biocompatibility.</p> <p>Results</p> <p>Novel 4, 4'-(1, 1'-(5-(2-methoxyphenoxy)-[2, 2'-bipyrimidine]-4, 6-diyl)bis(1<it>H</it>-pyrazol-3, 1-diyl)) dianiline fluorescent dye was synthesized by multistep synthesis from 2-phenylacetonitrile, 2-chloropyrimidine and 2-methoxyphenol. This dye has absorption at 379 nm with intense single emission at 497 nm having fairly good quantum yield (0.375) and Stokes shift. The intermediates and dye were characterized by FT-IR, ¹H NMR, ¹³C NMR and Mass spectral analysis. The pyrazole bipyrimidine based fluorescent dye possessing two amino groups suitable for binding with protein is reported. Its utility as a biocompatible conjugate was explained by conjugation with bovine serum albumin. The method is based on direct fluorescence detection of fluorophore-labelled protein before and after conjugation. Purified fluorescent conjugate was subsequently analyzed by fluorimetry. The analysis showed that the tested conjugation reaction yielded fluorescent conjugates of the dye through carbodiimide chemistry.</p> <p>Conclusion</p> <p>In summery synthesized fluorophore pyrazole-bipyrimidine has very good interaction towards protein bovine serum albumin and it acts as good candidate for protein assay.</p

THE PHARMACEUTICAL AND ANALYTICAL STUDY OF PALASH KSHARA

Background: Kshara is an important dosage form mentioned in Ayurveda which is derived from plant ash. The Kshara have some similar properties like alkaline nature, water solubility, whitish color etc. Kshara are given advantage over all surgical and parasurgical measures as it can be used in thin, weak and patients who fears for surgery. Different herbs like Apamarga, Snuhi, Kadalipalasha are used to prepare Kshara either single or collectively in classical texts. Therapeutic use of Kshara of different herbs differs from each other hence analytical study of each Kshara is necessary. Aims &amp; Objectives: In the present study preparation of Palash (Butea monosperma) kshara and analytical study is done to understand the characteristics of Palashkshara. Material &amp; methods: The preparation of Kshara is a multistep procedure. In the present study preparation of Palasha Kshara is done according general method of preparation of Kshara mentioned in Sharangadhara Sanhita. Prepared Kshara is subjected to various Physico chemical analyses and results are concluded. Results: The Palash Kshara shows physical properties like whitish in color, pungent odor and soft in touch. Chemically it shows combination of Potassium, Phosphate and Sulphate along with other elements in traces. The present study gives the details of methodology and results of the pharmaceutical and analytical study of Palash Kshara

PROVACATIVE POSITION WHILE NERVE CONDUCTION VELOCITY STUDY FOR DIAGNOSIS OF CARPAL TUNNEL SYNDROME

Research findings indicate that carpal tunnel syndrome (CTS) was a prevailing form of compressive neuropathy, resulting from the compression of the median nerve as it traverses the wrist within the CT. It constitutes 90% of all cases of entrapment neuropathies. In the majority of instances, both parties involved are impacted. The process of formulating a diagnosis is a cumulative one, relying on the evaluation of signs, symptoms, and specific physical tests. Confirmation of a suspected diagnosis may be achieved through the use of electrodiagnostic tests (EDT), specifically the Nerve Conduction Velocity Test(NCVT).Therefore, the objective of our study was to assess the relevance of the Provocative Position (PP) in conjunction with the NCVT for the diagnosis of CTS. We have conducted NCVT on a sample of 25 patients in our study. Additionally, the data was divided by the impulse latency in order to calculate the conduction velocity. Therefore, these steps were also replicated in the PP, and the resulting findings were subsequently compared. In our study, it was observed that bilateral tests exhibited the highest number of patients, with 10 individuals accounting for 40% of the sample. Additionally, 8 patients (32%) displayed left-side test results, while 7 patients (28%) exhibited right-side test results. The findings of this research indicate that there is a notable advantage in utilizing EDT investigations to promptly confirm suspected cases of CTS. This enables early intervention and alleviation of symptoms

Synthesis of novel dipodal-benzimidazole, benzoxazole and benzothiazole from cyanuric chloride: Structural, photophysical and antimicrobial studies

AbstractIn the present study, new benzimidazole, benzoxazole and benzothiazole derivatives were prepared and screened for antimicrobial activity. The structure of 4,4′-((6-(4-(diethylamino)phenyl)-1,3,5-triazine-2,4-diyl)bis(oxy))dibenzaldehyde (DIPOD) 5 was established from p-hydroxy benzaldehyde 4 and 4-(4,6-dichloro-1,3,5-triazin-2-yl)-N,N-diethylaniline 3. The reaction of DIPOD 5 with different o-phenylenediamine or o-amino phenol or o-amino thiophenol in ethanol gave benzimidazole, benzoxazole and benzothiazole 7. Novel heterocycles showed excellent broad-spectrum antimicrobial activity against bacterial strain (Escherichia coli, Staphylococcus aureus) and fungal strain (Candida albicans, Aspergillus niger) cultures. Activity data was compared with standard Streptomycin and Fluconazole drug. Photophysical and thermal properties of synthesized compounds were also studied

STUDIES ON GENETIC VARIABILITY IN DAHLIA (Dahlia variabilis L.)

ABSTRACT Twenty five dahlia accessions were evaluated to ascertain genetic parameters of variability. Phenotypic coefficients of variation (PCV) were higher than genotypic coefficients of variation (GCV) for all the characters studied. However higher PCV and GCV estimates were found for stalk length of the flower, duration of flowering, number of ray florets/flower and individual flower weight. High heritability with high genetic advance was observed for vase life, diameter of flower, stalk length of the flower and individual flower weight

Balancing glucose and oxygen uptake rates to enable high amorpha‐4,11‐diene production in Escherichia coli via the methylerythritol phosphate pathway

Amorpha‐4,11‐diene (AMD4,11) is a precursor to artemisinin, a potent antimalarial drug that is traditionally extracted from the shrubs of Artemisia annua. Despite significant prior efforts to produce artemisinin and its precursors through biotechnology, there remains a dire need for more efficient biosynthetic routes for its production. Here, we describe the optimization of key process conditions for an Escherichia coli strain producing AMD4,11 via the native methylerythritol phosphate (MEP) pathway. By studying the interplay between glucose uptake rates and oxygen demand, we were able to identify optimal conditions for increasing carbon flux through the MEP pathway by manipulating the availability of NADPH required for terpenoid production. Installation of an optimal qO2/qglucose led to a 6.7‐fold increase in product titers and a 6.5‐fold increase in carbon yield.Manus Bio, Inc.Projekt DEA

Leptin gene polymorphism in association with lactation milk yield in Murrah buffaloes

PCR amplification of exon-2 of leptin gene yielded an amplified product of 210 bp in Murrah buffaloes. RFLP of leptin gene done with Aci1 restriction enzyme revealed 3 types of genotypes CC, TC and TT in Murrah buffaloes of NDRI herd with a frequency of 0.26 , 0.73 and 0.013% respectively. Results suggested that CC genotype of leptin gene in Murrah buffaloes is significantly associated with 305 days milk yield. Overall study suggested the scope of considering genetic information of leptin gene in breeding and management strategies for the improvement of Murrah buffaloes of NDRI herd

Visible light-driven photocatalyst δ‑Bi7VO13 nanoparticles synthesized by thermal plasma

peer reviewedUnderstanding the electronic structure of photocatalysts is crucial for enhancing their efficiency. In this study, we have successfully synthesized novel monoclinic bismuth vanadate (Bi7VO13) nanoparticles using the gas phase condensation technique, with an average particle size of 40 nm. To investigate the crystallographic structure of the as-synthesized nanoparticles, we conducted X-ray diffraction (XRD) experiments. Additionally, we employed advanced characterization techniques to provide a detailed analysis of the electronic structure of Bi7VO13 nanoparticles. This study presents the first report on the electronic structure of Bi7VO13 nanoparticles using the aforementioned spectroscopic methods. Remarkably, the investigation revealed that the valence band maximum (VB) and conduction band minimum (CB) are dominated by O 2p and V 3d states, respectively. Moreover, X-ray absorption spectroscopy (XAS) reveals splitting the V 3d conduction band state into a triplet d-manifold at the V L-edge and O K-edge. This splitting arises from the lattice distortion induced by lone pairs, which gives rise to a band gap of 2.28 eV. Under visible light irradiation, the Bi7VO13 nanoparticles exhibit efficient visible light absorption, highlighting their potential for photocatalytic applications. Notably, our experiments demonstrated outstanding photodegradation properties of methylene blue, serving as a model effluent, further underscoring the photocatalytic progress of Bi7VO13 nanoparticles. In conclusion, this research explains the functioning of Bi7VO13 photocatalysts and opens the doors for utilizing their potential to generate a cleaner and brighter future