ANALYSIS OF BIOACTIVE CONSTITUENTS FROM A NEW STREPTOMYCES VARIABILIS STRAIN SU5 BY GAS CHROMATOGRAPHY - MASS SPECTROMETRY

Objectives: To analyze the bioactive constituents found in the crude extract of a new Streptomyces variabilis strain SU5 by gas chromatography - mass spectrometry. Methods: A total of fifty two actinomycetes were isolated from sediment samples of South east coast of Tamil Nadu by the serial dilution method. A new strain was identified based on cultural and molecular analysis as Streptomyces variabilis strain SU 5 and submitted in GENBANK with the accession number KF551876. The ethyl acetate extract of the new strain was produced by submerged fermentation and has been subjected to GC-MS analysis. Results: The major chemical constituents are Diisobutyl phthalate (31.84), Monoethylhexyl phthalate (20.33), tributylamine (13.24), Dibutyl phthalate (5.51), 2-Bromotetradecane (3.79) with the retention time 15.635, 21.606, 7.164, 16.608, 12.657 respectively. Conclusion: The presence of some of these constituents in the actinomycetes extract provides the scientific evidences for eliminating tumor cells in bone marrow, purging agent in autologous bone marrow transplantation, cytotoxic activity, stimulates adipogenesis and glyceroneogenesis, affects the differentiation of Human Liposarcoma