EVALUATION OF GASTROPROTECTIVE EFFECT OF COENZYME Q 10 AND L - GLUTAMINE IN STRESS AND ETHANOL-INDUCED PEPTIC ULCER IN WISTAR RATS: A NOVEL PRECLINICAL STUDY

Objective: Coenzyme Q 10 (CoQ10) and L-glutamine have antioxidant property their role in peptic ulcer diseases is not well known. Hence, the aim of present study is to evaluate the gastroprotective role of CoQ10 and L-glutamine in stress and ethanol-induced peptic ulcers in Wistar albino rats.Methods: A total of 90 rats were used for the conduct of study in two experimental models, the stress- and the ethanol-induced ulcer model. Each model consists of eight groups with six rats in each group. Sucralfate and pantoprazole were used as standard drugs along with CoQ10 and L- glutamine as test drugs. Drugs were administered for 10 days in stress model and 7 days in case of ethanol model. Statistically analyzed using analysis of variance and post-hoc test with significance as p>0.05Results: CoQ10 and L- glutamine when used alone as well in combination with pantoprazole and sucralfate showed no ulcer formation. Test drugs showed decrease in gastric acid secretion, decreased total and free acidity levels, higher gastric pH, increased mucous secreting ability, higher levels of reduced glutathione levels in both tissue as well as blood and lower malondialdehyde levels when compared with pantoprazole and sucralfate suggestive of their antioxidative benefit, in both the models.Conclusion: Well-designed clinical trials can be done to evaluate the use of CoQ10 and L- glutamine in the treatment of peptic ulcer disease due to various etiology

Comparative evaluation of postobturation apical seal following intracanal irrigation with maleic acid or a combination of chlorhexidine and ethylenediaminetetraacetic acid: An in vitro study

Aim: The aim of this study is to evaluate postobturation apical seal following intracanal irrigation with maleic acid (MA) or a combination of chlorhexidine (CHX) and ethylenediaminetetraacetic acid (EDTA). Materials and Methods: Thirty single-rooted human premolar teeth were decoronated and subjected to root canal instrumentation. Based on the final irrigating regimen, samples were divided into 3 groups: Group 1- Irritrol (EDTA + CHX), Group 2–7% MA, and Group 3–0.9% Saline (control). The samples were then obturated and placed in humidor for 7 days. Specimens were subjected to microleakage analysis at 24 h, 7 days, and 14 days using glucose filtration technique. The samples were then analyzed with a glucose kit in a calorimeter at 500 nm wavelength. Data were analyzed using one-way ANOVA, post hoc Tukey's test, post hoc Games–Howell test, and Bonferroni test. Results: There were overall significant differences in the mean scores among all the three groups (P < 0.001). Saline group demonstrated a significant higher leakage than that of MA followed by Irritrol. 14th day had significantly higher leakage when compared to 7th day with least being at 24 h. Conclusion: Final irrigation with Irritrol improved the postobturation apical seal when compared to 7% MA