GENOTOXICITY OF SHALLOW WATERS NEAR THE BRAZILIAN ANTARCTIC STATION "COMANDANTE FERRAZ" (EACF), ADMIRALTY BAY, KING GEORGE ISLAND, ANTARCTICA

Series of biomonitoring surveys were undertaken weekly in February 2012 to investigate the genotoxicity of the shallow waters around the Brazilian Antarctic Station "Comandante Ferraz" (EACF). The comet assay was applied to assess the damage to the DNA of hemocytes of the crustacean amphipods Gondogeneia antarctica collected from shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) of the research station, and compare it to the DNA damage of animals from Punta Plaza (PPL) and Yellow Point (YP), natural sites far from the EACF defined as experimental controls. The damage to the DNA of hemocytes of G. antarctica was not significantly different between sites in the biomonitoring surveys I and II. In survey III, the damage to the DNA of animals captured in shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) was significantly higher than that of the control site of Punta Plaza (PPL). In biomonitoring survey IV, a significant difference was detected only between the FT and PPL sites. Results demonstrated that the shallow waters in front of the station may be genotoxic and that the comet assay and hemocytes of G. antarctica are useful tools for assessing genotoxicity in biomonitoring studies of Antarctic marine coastal habitats.Séries de biomonitoramentos foram executadas semanalmente, durante o mês de fevereiro de 2012, para se investigar a genotoxicidade de ambientes costeiros rasos no entorno da Estação Antártica "Comandante Ferraz (EACF). O ensaio cometa foi aplicado para se avaliar os danos ao DNA hemocitário de crustáceos anfípodes Gondogeneia antarctica coletados em áreas rasas próximas aos tanques de armazenamento de combustível (FT) e saída de efluentes da estação de tratamento de esgoto (STO), em comparação aos danos ao DNA de animais provenientes de Punta Plaza (PPL) e Yellow Point (YP), locais naturais distantes da EACF, definidos como controles experimentais. O dano ao DNA hemocitário de G. antarctica não foi significativamente diferente entre locais, nos biomonitoramentos I e II. No biomonitoramento III, o dano ao DNA de animais coletados em águas rasas próximas aos tanques de combustível e saída de efluentes de esgoto foi significativamente maior do que aquele do controle de Punta Plaza. No biomonitoramento IV, a diferença foi significativa somente entre os locais dos tanques de combustível e de Punta Plaza. Estes resultados demonstram que a contaminação das águas em frente à EACF pode ser genotóxica e que, tanto o ensaio cometa quanto os hemócitos de G. antarctica são ferramentas úteis na avaliação da genotoxicidade em estudos de biomonitoramento de habitats marinhos costeiros da Antártica

Assessment of genotoxicity and depuration of anthracene in the juvenile coastal fish Trachinotus carolinus using the comet assay

Ambientalmente, o antraceno é considerado persistente, bioacumulativo e tóxico para organismos aquáticos. A biotransformação de substâncias xenobióticas, como o antraceno, produz espécies reativas de oxigênio que podem agir sobre o DNA. O objetivo deste trabalho foi avaliar o dano ao DNA em T. carolinus expostos a diferentes concentrações de antraceno (8, 16 e 32 µg.L-1) por 24 h no escuro e subsequentemente mantidos em água limpa para depuração por três diferentes períodos de tempo (48, 96 e 144 h) através de ensaio cometa. Os resultados obtidos demonstram que o antraceno é genotóxico para T. carolinus e o dano ao DNA foi dose- e tempo de depuração- dependente. A genotoxicidade do antraceno foi detectada nas três concentrações utilizadas. A depuração pareceu ser mais eficiente nos peixes expostos a menor concentração de antraceno e mantidos por 96 h em água limpa.In the environment, anthracene is characterized as being persistent, bioaccumulative and toxic to aquatic organisms. Biotransformation of xenobiotic substances, such as anthracene, produces reactive oxygen species that may induce DNA strand breaks. The aim of the present study was to evaluate the DNA damage in juvenile T. carolinus exposed to different concentrations (8, 16 and 32 µg.L-1) of anthracene for 24 h in the dark then subsequently allowed to depurate in clean water for different periods of time (48, 96 or 144 h) using the comet assay. Our results show that anthracene is genotoxic to T. carolinus and that DNA damage was dose- and depuration/time- dependent. Anthracenegenotoxicity was observed in all experimental concentrations. Depuration seemed to be more efficient in fish exposed to thelowest anthracene concentration and maintained in clean water for 96 h

Autolesão não suicida na adolescência como fator de predisposição ao suicídio

A autolesão não suicida na adolescência é um evento frequente e está associada a tentativas subsequentes de suicídio ou ao suicídio completo, sugerindo que esse comportamento e aspectos psicológicos relacionados a ele possam estar na mesma trajetória de risco. As regras legais determinam a obrigação do médico notificar casos suspeitos ou confirmados de automutilação às autoridades de saúde. No entanto, não se trata apenas de cumprir com os requisitos legais; o médico deve estar ciente dessa possibilidade, conhecendo os mecanismos aplicados pelos adolescentes, sabendo os motivos que possam desencadear esse comportamento prejudicial e, consequentemente, adotar medidas preventivas eficazes. De maneira geral, os adolescentes relatam que a autolesão alivia o sentimento de angústia, culpa ou vergonha, sendo uma maneira de autopunição. Embora a autolesão não-suicida geralmente resulte em alívio momentâneo da angústia, frequentemente leva a consequências negativas a longo prazo. Este estudo narrativo teve como objetivo mostrar os principais tópicos relacionados a este grave e prevalente problema.Non-suicidal self-injury is frequent among adolescents and is associated with subsequent attempted and completed suicides, suggesting that these behaviors and their related psychology may be on the same risk trajectory. Legal rules determine the doctor's obligation to notify suspected or confirmed cases of self-harm to health authorities. However, it is not just a matter of complying with the legal requirements; the doctor must be aware of this possibility, knowing how adolescents function and what might trigger harmful behavior to be able to adopt effective preventive measures. In general, adolescents report that self-harm relieves angst, guilt, or shame as a form of self-punishment. While non-suicidal self-injury often momentarily relieves anxiety, it frequently leads to long-term negative consequences. This narrative study aimed to point out the main topics related to this grave and highly prevalent problem

Massive coastal tourism influx to the Mediterranean Sea: The environmental risk of sunscreens

The Mediterranean region is, by far, the leading tourism destination in the world, receiving more than 330 million tourists in 2016. This tourism is undertaken mostly for seaside holidays, and during the summer season concentrates between 46% and 69% of the total international arrivals; this is equivalent to a density of 2.9 tourists per meter of Mediterranean coast, or double this number taking into account the local/permanent population in addition. Previous studies have reported not only the presence of sunscreen in the various environmental compartments (water, sediments and biota) of the Mediterranean Sea (MS) and other regions, but also show that sunscreen products are toxic for marine biota and are accumulated and biomagnificated. Here, we highlight that the environmental risk of these chemicals is likely to be exacerbated in the MS due to the massive influx of tourists and its densely populated coasts, the basin's limited exchanges with the ocean, the high residence time of surface waters, and its oligotrophic waters.A. Rodríguez-Romero is supported by the Spanish grant Juan de la Cierva Formación 2015 (JCI-2015-26873) and thanks to the University of Cantabria “Proyecto Puente 2017”under SODERCAN and ERDF Operational Programme

GENOTOXICITY OF SHALLOW WATERS NEAR THE BRAZILIAN ANTARCTIC STATION "COMANDANTE FERRAZ" (EACF), ADMIRALTY BAY, KING GEORGE ISLAND, ANTARCTICA

Series of biomonitoring surveys were undertaken weekly in February 2012 to investigate the genotoxicity of the shallow waters around the Brazilian Antarctic Station "Comandante Ferraz" (EACF). The comet assay was applied to assess the damage to the DNA of hemocytes of the crustacean amphipods Gondogeneia antarctica collected from shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) of the research station, and compare it to the DNA damage of animals from Punta Plaza (PPL) and Yellow Point (YP), natural sites far from the EACF defined as experimental controls. The damage to the DNA of hemocytes of G. antarctica was not significantly different between sites in the biomonitoring surveys I and II. In survey III, the damage to the DNA of animals captured in shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) was significantly higher than that of the control site of Punta Plaza (PPL). In biomonitoring survey IV, a significant difference was detected only between the FT and PPL sites. Results demonstrated that the shallow waters in front of the station may be genotoxic and that the comet assay and hemocytes of G. antarctica are useful tools for assessing genotoxicity in biomonitoring studies of Antarctic marine coastal habitats

Electronic word of mouth communications and consumer experience : an evaluation of pre- and post- purchase responses and reactions to verbal and visual online reviews

Increased Internet access and consequent use of electronic word of mouth ( e WO M) has re­ defined the word of mouth (WOM) landscape with communication coming increasingly from weak tie sources, as consumers share verbal (written reviews) and visual (photos) content. This online information exchange is frequent but lacks the physical cues usually found in face-to-face interactions. As a result, the process of evaluation of the perceived helpfulness of pre-and post-purchase eWOM differs from that of its traditional offline sibling. This thesis researches how consumers interact with eWOM relating to hotel choice before and after a booking is made. It investigates consumers' eWOM processing of, both verbal and visual; the development of consumers' perceptions of helpfulness of eWOM over time, and consumers' eWOM communication behaviour in a three-study staged process. The concepts underpinning this research are related to functional and hedonic aspects of information processing; and approach-avoidance theory. A multi-method approach is adopted in which consumers' interactions with pre-and post-purchase eWOM are examined qualitatively (Studies 1 and 2); with post-purchase interactions also studied quantitatively (Study 3). Thematic analysis of Study I indicates multiple roles of the aspects of eWOM (i.e. valence, volume, temporal cue, consensus, homophily, fairness, completeness, relevance and accuracy). Consumers might use them to: (1) perceive trustworthiness; (2) to save-time; and (3)to interpret the helpfulness of eWOM. Their interpretation of helpfulness is informed by their perceived fairness and relevance of verbal and visual eWOM. The longitudinal element of Study 2 subsequently identifies the fluid and complex development of these perceptions (i.e. fairness and relevance) over time (between pre-and post-consumption) for verbal and visual eWOM. Study 2 also untangles the different meanings given to motivators of eWOM communication and identifies a number of elements discouraging eWOM production. Study 3 develops specific measures of these inhibitors using a well-accepted methodology. This research makes several distinct contributions: Study 1 advances knowledge by combining functional and hedonic elements of information processing and develops the previously understudied concepts of fairness and subjective aspects of homophily. It also explores the roles of visual eWOM on information processing. The major contributions of Study 2 are identifying changes in perceptions of eWOM over time and to expand understanding of the positive and negative issues affecting eWOM production post-purchase. Previous research has focused on motivators; inhibitors by contrast have received little attention. Study 3 addresses this omission by developing specific measures to assess the effects of inhibitors since no scales currently exist. If marketers more fully understand the reasons why consumers fail to produce WOM, they can develop strategies to reduce the imbalance between widespread eWOM use and its restricted production

Ecological implications of copper-based nanoparticles in aquatic complex matrices: Fate, behavior, and toxicity assessment

Engineered nanomaterials (ENMs) are likely to undergo some degree of modification when released into the environment, which can influence the fate, behavior, and toxicity of nanoparticles (NPs). The environmental factors in natural aquatic ecosystems, such as water chemistry, hydrology, disturbance, and biotic interactions, can transform or “age” toxic chemicals through physical, chemical, and biological processes, including aggregation and disaggregation, adsorption, redox reaction, dissolution, complexation and biotransformation. The extent of aging can vary considerably over time and within a single or a number of water bodies, such as a river that flows into the ocean through an estuary. However, the ecological effects of NPs under realistic environmental exposure scenarios are not yet fully understood. Adding to these challenges are problems arising from traditional ecotoxicological risk assessments, which are inevitably hampered by narrow subsets of relevant species, toxicants, exposure conditions, and levels of impact. The present work examined the toxicity of copper-based nanoparticles (CBNPs), which frequently enter natural aquatic ecosystems due to their increasing application in consumer products, by assessing their impact on marine phytoplankton and estuarine amphipods, organisms that are central to aquatic ecosystems. Standard toxicological methods were used, along with physiological measurements, studies of fate and transport, and mechanistic biological models based on Dynamic Energy Budget (DEB) theory. The aim of the work was to understand 1) the influence of aging processes on CBNPs under environmentally relevant test conditions, 2) the impact of aged CBNPs on a marine phytoplankton population, 3) the potential impact of CBNPs on non-target estuarine organisms, and 4) the potential for detecting and predicting the toxic effects of CBNPs on an individual, to generate model estimates of effects on populations and communities. CBNPs were found to be toxic to benthic estuarine organisms at concentrations of CBNPs already found in the natural environment. However, sublethal toxicity may not be detected by traditional ecotoxicological tests. Additionally, aging was found to influence the fate and transport of CBNPs through oxidation, aggregation, and dissolution processes, increasing Cu toxic ion bioavailability to pelagic organisms over time. While current studies increasingly consider more realistic environmental exposure scenarios, this work, as well as that of other researchers, suggests that CBNPs behave differently under prolonged environmental exposure, and nanoecotoxicological research should focus on sublethal impacts, integrated with mechanistic biological models and based on Dynamic Energy Budget (DEB) theory

Study on the genotoxicity of titanium dioxide nanoparticle in juvenille pompano, Trachinotus carolinus (Linnaeus, 1766), using cytogenotoxic methods

Nanopartículas possuem características físico-químicas úteis para o uso humano, com aplicação na ciência, tecnologia, medicina e produtos de uso diário. A fabricação e a variedade destes produtos vêm crescendo rapidamente, gerando uma preocupação quanto ao risco de exposição aos efeitos tóxicos da contaminação por nanopartículas. Vários efeitos adversos, derivados da exposição de nanopartículas foram descritos tanto para organismos terrestres como aquáticos, porém efeitos genotóxicos ainda são pouco conhecidos para organismos marinhos. Para avaliar a citogenotoxicidade da nanopartícula de dióxido de titânio no peixe marinho T. carolinus, foram administradas injeções intraperitoneais com dosagens de 1,5 e 3,0 ?g-nano-TiO2/grama-de-peixe. Amostras de sangue foram coletadas para realização do teste de micronúcleo (MN) e outras anormalidades nucleares eritrocitárias (ANE) e ensaio cometa (pH>13), 24, 48 e 72 horas após a injeção. Foram investigadas a viabilidade de eritrócitos e a possibilidade de acumulação da nano-TiO2 nos tecidos dos peixes injetados com a maior dosagem, após 72 horas, pelo teste de exclusão do azul de tripano e por microscopia eletrônica de transmissão. Os resultados mostraram que a nano-TiO2 é uma substância possivelmente capaz de entrar nas células e induzir efeito genotóxico e citotóxico para esta espécie, por provocar uma diminuição na viabilidade de eritrócitos, um aumento no dano ao DNA e formação de MN e outras ANE. De acordo com nosso conhecimento, este é o único trabalho sobre a genotoxicidade de nano-TiO2 em um peixe marinho.Nanoparticles have physicochemical characteristics useful for human use, with applications in science, technology, medicine and everyday products. The manufacture and variety of these products have been growing rapidly, creating a concern about the risk of exposure to the toxic effects of contamination by nanoparticles. Several adverse effects of exposure to nanoparticles have been reported for both terrestrial and aquatic organisms, but genotoxic effects are still poorly known for marine organisms. To evaluate the citogenotoxicity of titanium dioxide nanoparticles (nano-TiO2) in marine fish T. carolinus, the substance was administered with intraperitoneal injections at dosages of 1,5 and 3,0 ?g-nano-TiO2/gram-of-fish. Blood samples were collected for the tests of micronucleus (MN) and other erythrocytes nuclear abnormalities (ENA) and comet assay (pH> 13), 24, 48 and 72 hours after injection. We also investigated the viability of erythrocytes and the possibility of accumulation of nano-TiO2 in the tissues of fish injected with the highest dose after 72 hours using trypan blue exclusion test and transmission electron microscopy. The results showed that the nano-TiO2 is a substance that can possibly be able to enter cells and induce cytotoxic and genotoxic effect for this species, by causing a decrease in the viability of erythrocytes, an increase in DNA damage and formation of MN and other ANE. As far as we know, this is the only work done on the genotoxicity of nano-TiO2 in a marine fis

Ecological implications of copper-based nanoparticles in aquatic complex matrices: Fate, behavior, and toxicity assessment

Engineered nanomaterials (ENMs) are likely to undergo some degree of modification when released into the environment, which can influence the fate, behavior, and toxicity of nanoparticles (NPs). The environmental factors in natural aquatic ecosystems, such as water chemistry, hydrology, disturbance, and biotic interactions, can transform or “age” toxic chemicals through physical, chemical, and biological processes, including aggregation and disaggregation, adsorption, redox reaction, dissolution, complexation and biotransformation. The extent of aging can vary considerably over time and within a single or a number of water bodies, such as a river that flows into the ocean through an estuary. However, the ecological effects of NPs under realistic environmental exposure scenarios are not yet fully understood. Adding to these challenges are problems arising from traditional ecotoxicological risk assessments, which are inevitably hampered by narrow subsets of relevant species, toxicants, exposure conditions, and levels of impact. The present work examined the toxicity of copper-based nanoparticles (CBNPs), which frequently enter natural aquatic ecosystems due to their increasing application in consumer products, by assessing their impact on marine phytoplankton and estuarine amphipods, organisms that are central to aquatic ecosystems. Standard toxicological methods were used, along with physiological measurements, studies of fate and transport, and mechanistic biological models based on Dynamic Energy Budget (DEB) theory. The aim of the work was to understand 1) the influence of aging processes on CBNPs under environmentally relevant test conditions, 2) the impact of aged CBNPs on a marine phytoplankton population, 3) the potential impact of CBNPs on non-target estuarine organisms, and 4) the potential for detecting and predicting the toxic effects of CBNPs on an individual, to generate model estimates of effects on populations and communities. CBNPs were found to be toxic to benthic estuarine organisms at concentrations of CBNPs already found in the natural environment. However, sublethal toxicity may not be detected by traditional ecotoxicological tests. Additionally, aging was found to influence the fate and transport of CBNPs through oxidation, aggregation, and dissolution processes, increasing Cu toxic ion bioavailability to pelagic organisms over time. While current studies increasingly consider more realistic environmental exposure scenarios, this work, as well as that of other researchers, suggests that CBNPs behave differently under prolonged environmental exposure, and nanoecotoxicological research should focus on sublethal impacts, integrated with mechanistic biological models and based on Dynamic Energy Budget (DEB) theory