Coronavírus canino (ccov): isolamento e detecção molecular em amostras clínicas

Until now, the disease caused by the canine coronavirus (CCoV) was not fully elucidated, the CCoV role in the pathogenesis of the illness is not quite established. Only after the SARS (Severe Acute Respiratory Syndrome) appearance in human beings caused by a coronavirus in 2002, isolated of a wild mammal, many studies were developed with coronavirus of different species in search of circulating variants. Few studies had reported the presence of CCoV and his variability's in Brazil. Viral enteritis are responsible by high morbidity taxes and mortality in the small animals clinic and, although there are several etiologic agents described, the CCoV and the CPV are not yet considered a major cause of acute gastroenteritis pathogens in young dogs. For the present study, were collected 100 stool samples from dogs attended in a veterinary hospital routine with clinical signs of gastroenteritis (hemorrhagic or not). The CCoV and CPV RNA were amplified in the tested samples. Some of the by-products from the CPV amplification were purified, sequenced, aligned and subjected to the GenBank for the detection of CPV-2c and characterization of CCoV. The CCoV was present in 6% of the analyzed samples, while CPV-2a was identified in 43% of samples and CPV-2b in 18% of the total. A sample pool submitted to the sequencing and filogenetic analysis identified the CPV-2c, reveling that the new variant is already present in Araçatuba - SP. The phylogenetic analyses for CCoV revealed that the studied samples belong to the Subtype CCoV-IIa (pantropic), strengthening the agent identification importance in the canine population, once that the actual available vaccines don't have the new variants so that the dogs from Brazil are susceptible to severe outbreaks, as described in other countries.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Estimation of the diagnostic accuracy of the glyco-C and US9 gene-based polymerase chain reaction technique for the detection of bovine Herpesvirus type 5 DNA in decomposed brain suspension from a slaughter house using Bayesian analysis, Brazil

Brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. The goal of this study was to estimate the accuracy of the glycol and US9 gene-based polymerase chain reactions (PCRs) for the detection of bovine Herpesvirus type 5 (BoHV-5) from decomposed brain samples (n = 95). For this purpose, a latent-class (bayesian) approach was used. Sensitivity (Se) was estimated to be 70% (95% probability interval, 40-80) and specificity (Sp) 100% in the statistical analysis for both PCRs used. Accordingly, a minimum of ≥40% of the calves was estimated to harbor BoHV-5 DNA even after 72 h of decomposition at room temperature. It was concluded that US9 gene-based PCR could also be considered a cost-effective alternative in sanitary programmers. However, given the importance of veterinary diagnoses, PCR-positive samples should be further confirmed through in vitro isolation and/or sequencing.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Resumos em andamento - Saúde Coletiva

Resumos em andamento - Saúde Coletiv

Resumos em andamento - Saúde Coletiva

Resumos em andamento - Saúde Coletiv