3 research outputs found
ΠΠ¦ΠΠΠΠ ΠΠΠΠ’ΠΠ ΠΠΠ¦ΠΠΠΠΠΠΠΠΠ‘Π’Π ΠΠΠΠΠΠΠΠΠ«Π₯ ΠΠΠΠΠ§ΠΠΠΠΠ‘ΠΠ«Π₯ ΠΠΠΠ’ΠΠ ΠΠ, ΠΠΠΠ£Π§ΠΠΠΠ«Π₯ Π‘ Π’Π£Π¨ ΠΠ Π£ΠΠΠΠΠ Π ΠΠΠΠ’ΠΠΠ Π‘ΠΠΠ’Π
Cattle carcasses of ritual slaughter (Halal) from the lateral and medial side were investigated in order to identify the indigenous strains of lactic acid microorganisms capable of producing bacteriocins. Only 14 strains, from 36 strains of microorganisms isolated from washings taken from carcasses, were used for further research and identified as lactabacilus. In the study of their bacteriocinogenicity by the method of two-layer agars of modified composition, high inhibitory efficiency was proved with respect to the growth of the pathogen Salmonella typhimurium, only 6 isolated strains of lactabacilus. The use of this method of evaluation will not only reveal the presence of the ability of the lactabacilus to produce bacteriocins, but also to study their antagonistic activity against many of the studied microorganisms, which are indicators of compliance with various modes of technological processes. The presence of such microorganisms in the meat will increase the shelf life of meat, due to the suppression of the growth of closely related lactabacilus, some of which are spoilage microorganisms, as well as the growth of opportunistic and pathogenic microflora. In addition, the use of lactabacilus with bacteriocinogenicity in the production technology of raw sausages will reduce the risk of production of unsafe products. Thus, the presence of indigenous lactabacilus in meat is an additional factor that ensures the flow of desired biochemical processes and the safety of raw smoked sausages.ContributionAll authors bear responsibility for the work and presented data. All authors made an equal contribution to the work. Dagmara S. Bataeva developed scientific and methodological approaches to work, determined the scope of research, analyzed the data obtained, performed the narrative and corrected it after submitting to the editorial office. Olga V. Sokolova conducted a review and analysis of the literature, carried out the descriptive part Elena V. Zaiko selected research objects, carried out microbiological analysis. Victoria V. Pashkova carried out preparatory work and microbiological analysis. The authors were equally involved in writing the manuscript and bear the equal responsibility for plagiarism.Conflict of interestThe authors declare no conflict of interest.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ ΡΡΡΠΈ ΠΊΡΡΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΠ³Π°ΡΠΎΠ³ΠΎ ΡΠΊΠΎΡΠ° ΡΠΈΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ±ΠΎΡ (Ρ
Π°Π»ΡΠ»Ρ) ΡΒ Π»Π°ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈΒ ΠΌΠ΅Π΄ΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠΎΡΠΎΠ½Ρ ΡΒ ΡΠ΅Π»ΡΡ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΠΈΠ½Π΄ΠΈΠ³Π΅Π½Π½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΊΠΈΡΠ»ΡΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΡΠΏΠΎΡΠΎΠ±Π½ΡΡ
ΠΏΡΠΎΠ΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠΈΠ½Ρ. ΠΠ·Β 36Β ΡΡΠ°ΠΌΠΌΠΎΠ² ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΠΈΠ· ΡΠΌΡΠ²ΠΎΠ², ΠΎΡΠΎΠ±ΡΠ°Π½Π½ΡΡ
ΡΒ ΡΡΡ, ΡΠΎΠ»ΡΠΊΠΎ 14 Π±ΡΠ»ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ Π΄Π»Ρ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈΒ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΊΠΈΡΠ»ΡΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΈ (ΠΠΠ). ΠΡΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠΈ ΠΈΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠΈΠ½ΠΎΠ³Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΠΎ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠ΅ Π΄Π²ΡΡ
ΡΠ»ΠΎΠΉΠ½ΡΡ
Π°Π³Π°ΡΠΎΠ² ΠΌΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π°, Π±ΡΠ»Π° Π΄ΠΎΠΊΠ°Π·Π°Π½Π° Π²ΡΡΠΎΠΊΠ°Ρ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ°Ρ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²Β ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΡΠΎΡΡΠ° ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ° Salmonella typhimurium, ΡΠΎΠ»ΡΠΊΠΎ 6 Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΡΡΠ°ΠΌΠΌΠΎΠ² ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΊΠΈΡΠ»ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅ΡΠΎΠ΄Π° ΠΎΡΠ΅Π½ΠΊΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ Π½Π΅ ΡΠΎΠ»ΡΠΊΠΎ Π²ΡΡΠ²ΠΈΡΡ Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΠΏΠΎΡΠΎΠ±Π½ΠΎΡΡΠΈ ΠΠΠ ΠΏΡΠΎΠ΄ΡΡΠΈΡΠΎΠ²Π°ΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠΈΠ½Ρ, Π½ΠΎ ΠΈΒ ΠΈΠ·ΡΡΠΈΡΡ ΠΈΡ
Π°Π½ΡΠ°Π³ΠΎΠ½ΠΈΡΡΠΈΡΠ΅ΡΠΊΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π²Β ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΈΡ
ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ²Π»ΡΡΡΡΡ ΠΈΠ½Π΄ΠΈΠΊΠ°ΡΠΎΡΠ°ΠΌΠΈ ΡΠΎΠ±Π»ΡΠ΄Π΅Π½ΠΈΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠ΅ΠΆΠΈΠΌΠΎΠ² ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ². ΠΠ°Π»ΠΈΡΠΈΠ΅ ΡΠ°ΠΊΠΈΡ
ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π²Β ΠΌΡΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ ΡΠ²Π΅Π»ΠΈΡΠΈΡΡ ΡΡΠΎΠΊ Ρ
ΡΠ°Π½Π΅Π½ΠΈΡ ΠΌΡΡΠ°, Π·Π° ΡΡΠ΅Ρ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΡ ΡΠΎΡΡΠ° Π±Π»ΠΈΠ·ΠΊΠΎΡΠΎΠ΄ΡΡΠ²Π΅Π½Π½ΡΡ
Π»Π°ΠΊΡΠΎΠ±Π°ΡΠΈΠ»Π», Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΈΠ· ΠΊΠΎΡΠΎΡΡΡ
ΡΠ²Π»ΡΡΡΡΡ ΠΌΠΈΠΊΡΠΎΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ ΠΏΠΎΡΡΠΈ, Π°Β ΡΠ°ΠΊΠΆΠ΅ ΡΠΎΡΡ ΡΡΠ»ΠΎΠ²Π½ΠΎ-ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΈΒ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ. ΠΡΠΎΠΌΠ΅ ΡΡΠΎΠ³ΠΎ, ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΊΠΈΡΠ»ΡΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΡ
Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΠΈΠ½ΠΎΠ³Π΅Π½Π½ΠΎΡΡΡΡ, Π²Β ΡΠ΅Ρ
Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° ΡΡΡΠΎΠΊΠΎΠΏΡΠ΅Π½ΡΡ
ΠΊΠΎΠ»Π±Π°Ρ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ ΡΠ½ΠΈΠ·ΠΈΡΡ ΡΠΈΡΠΊ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²Π° Π½Π΅Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΠΉ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π½Π°Π»ΠΈΡΠΈΠ΅ Π²Β ΠΌΡΡΠ΅ ΠΈΠ½Π΄ΠΈΠ³Π΅Π½Π½ΡΡ
ΠΠΠ ΡΠ²Π»ΡΠ΅ΡΡΡ Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠΈΠΌ ΠΏΡΠΎΡΠ΅ΠΊΠ°Π½ΠΈΠ΅ ΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΡΡ
Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΠΈΒ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΡΡΡ ΡΡΡΠΎΠΊΠΎΠΏΡΠ΅Π½ΡΡ
ΠΊΠΎΠ»Π±Π°Ρ.ΠΡΠΈΡΠ΅ΡΠΈΠΈ Π°Π²ΡΠΎΡΡΡΠ²Π°ΠΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎΡΡΡ Π·Π° ΡΠ°Π±ΠΎΡΡ ΠΈΒ ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ Π½Π΅ΡΡΡ Π²ΡΠ΅ Π°Π²ΡΠΎΡΡ. ΠΡΠ΅ Π°Π²ΡΠΎΡΡ Π²Β ΡΠ°Π²Π½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠ°ΡΡΠ²ΠΎΠ²Π°Π»ΠΈ Π²Β ΡΡΠΎΠΉ ΡΠ°Π±ΠΎΡΠ΅. ΠΠ°ΡΠ°Π΅Π²Π° Π.Π‘. ΡΠ°Π·ΡΠ°Π±Π°ΡΡΠ²Π°Π»Π° Π½Π°ΡΡΠ½ΠΎ-ΠΌΠ΅ΡΠΎΠ΄ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Ρ ΠΊΒ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΡΠ°Π±ΠΎΡ, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»Π° ΠΎΠ±ΡΠ΅ΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅, Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π° ΠΎΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΡ ΡΠ°ΡΡΡ ΡΡΠ°ΡΡΠΈ ΠΈΒ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»Π° ΠΏΠΎΡΠ»Π΅ ΠΏΠΎΠ΄Π°ΡΠΈ Π²Β ΡΠ΅Π΄Π°ΠΊΡΠΈΡ Π‘ΠΎΠΊΠΎΠ»ΠΎΠ²Π° Π.Π. ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΎΠ±Π·ΠΎΡ ΠΈΒ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ, Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π° ΠΎΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΡ ΡΠ°ΡΡΡ ΠΠ°ΠΉΠΊΠΎ Π.Π. ΠΎΡΠ±ΠΈΡΠ°Π»Π° ΠΎΠ±ΡΠ΅ΠΊΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ, Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π° ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ·. ΠΠ°ΡΠΊΠΎΠ²Π° Π.Π. Π²ΡΠΏΠΎΠ»Π½ΡΠ»Π° ΠΏΠΎΠ΄Π³ΠΎΡΠΎΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΠ°Π±ΠΎΡΡ, ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΠ²ΡΠΎΡΡ Π²Β ΡΠ°Π²Π½ΡΡ
Π΄ΠΎΠ»ΡΡ
ΠΈΠΌΠ΅ΡΡ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ ΠΊΒ Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΡ ΡΡΠΊΠΎΠΏΠΈΡΠΈ ΠΈΒ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΠΎ Π½Π΅ΡΡΡ ΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎΡΡΡ Π·Π° ΠΏΠ»Π°Π³ΠΈΠ°Ρ.ΠΠΎΠ½ΡΠ»ΠΈΠΊΡ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠΎΠ²ΠΠ²ΡΠΎΡΡ Π·Π°ΡΠ²Π»ΡΡΡ ΠΎΠ± ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ ΠΊΠΎΠ½ΡΠ»ΠΈΠΊΡΠ° ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠΎΠ²
ASSESSMENT OF THE BACTERIOCINOGENICITY OF INDIGEN LACTOBACILLUS ONTO CATTLE CARCASSES
Cattle carcasses of ritual slaughter (Halal) from the lateral and medial side were investigated in order to identify the indigenous strains of lactic acid microorganisms capable of producing bacteriocins. Only 14 strains, from 36 strains of microorganisms isolated from washings taken from carcasses, were used for further research and identified as lactabacilus. In the study of their bacteriocinogenicity by the method of two-layer agars of modified composition, high inhibitory efficiency was proved with respect to the growth of the pathogen Salmonella typhimurium, only 6 isolated strains of lactabacilus. The use of this method of evaluation will not only reveal the presence of the ability of the lactabacilus to produce bacteriocins, but also to study their antagonistic activity against many of the studied microorganisms, which are indicators of compliance with various modes of technological processes. The presence of such microorganisms in the meat will increase the shelf life of meat, due to the suppression of the growth of closely related lactabacilus, some of which are spoilage microorganisms, as well as the growth of opportunistic and pathogenic microflora. In addition, the use of lactabacilus with bacteriocinogenicity in the production technology of raw sausages will reduce the risk of production of unsafe products. Thus, the presence of indigenous lactabacilus in meat is an additional factor that ensures the flow of desired biochemical processes and the safety of raw smoked sausages.ContributionAll authors bear responsibility for the work and presented data. All authors made an equal contribution to the work. Dagmara S. Bataeva developed scientific and methodological approaches to work, determined the scope of research, analyzed the data obtained, performed the narrative and corrected it after submitting to the editorial office. Olga V. Sokolova conducted a review and analysis of the literature, carried out the descriptive part Elena V. Zaiko selected research objects, carried out microbiological analysis. Victoria V. Pashkova carried out preparatory work and microbiological analysis. The authors were equally involved in writing the manuscript and bear the equal responsibility for plagiarism.Conflict of interestThe authors declare no conflict of interest