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    ΠžΠ¦Π•ΠΠšΠ Π‘ΠΠšΠ’Π•Π Π˜ΠžΠ¦Π˜ΠΠžΠ“Π•ΠΠΠžΠ‘Π’Π˜ Π˜ΠΠ”Π˜Π“Π•ΠΠΠ«Π₯ ΠœΠžΠ›ΠžΠ§ΠΠžΠšΠ˜Π‘Π›Π«Π₯ Π‘ΠΠšΠ’Π•Π Π˜Π™, ΠŸΠžΠ›Π£Π§Π•ΠΠΠ«Π₯ Π‘ Π’Π£Π¨ ΠšΠ Π£ΠŸΠΠžΠ“Πž Π ΠžΠ“ΠΠ’ΠžΠ“Πž БКОВА

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    Cattle carcasses of ritual slaughter (Halal) from the lateral and medial side were investigated in order to identify the indigenous strains of lactic acid microorganisms capable of producing bacteriocins. Only 14 strains, from 36 strains of microorganisms isolated from washings taken from carcasses, were used for further research and identified as lactabacilus. In the study of their bacteriocinogenicity by the method of two-layer agars of modified composition, high inhibitory efficiency was proved with respect to the growth of the pathogen Salmonella typhimurium, only 6 isolated strains of lactabacilus. The use of this method of evaluation will not only reveal the presence of the ability of the lactabacilus to produce bacteriocins, but also to study their antagonistic activity against many of the studied microorganisms, which are indicators of compliance with various modes of technological processes. The presence of such microorganisms in the meat will increase the shelf life of meat, due to the suppression of the growth of closely related lactabacilus, some of which are spoilage microorganisms, as well as the growth of opportunistic and pathogenic microflora. In addition, the use of lactabacilus with bacteriocinogenicity in the production technology of raw sausages will reduce the risk of production of unsafe products. Thus, the presence of indigenous lactabacilus in meat is an additional factor that ensures the flow of desired biochemical processes and the safety of raw smoked sausages.ContributionAll authors bear responsibility for the work and presented data. All authors made an equal contribution to the work. Dagmara S. Bataeva developed scientific and methodological approaches to work, determined the scope of research, analyzed the data obtained, performed the narrative and corrected it after submitting to the editorial office. Olga V. Sokolova conducted a review and analysis of the literature, carried out the descriptive part Elena V. Zaiko selected research objects, carried out microbiological analysis. Victoria V. Pashkova carried out preparatory work and microbiological analysis. The authors were equally involved in writing the manuscript and bear the equal responsibility for plagiarism.Conflict of interestThe authors declare no conflict of interest.Π˜ΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ‹ Ρ‚ΡƒΡˆΠΈ ΠΊΡ€ΡƒΠΏΠ½ΠΎΠ³ΠΎ Ρ€ΠΎΠ³Π°Ρ‚ΠΎΠ³ΠΎ скота Ρ€ΠΈΡ‚ΡƒΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ убоя (Ρ…Π°Π»ΡΠ»ΡŒ) ΡΒ Π»Π°Ρ‚Π΅Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ и мСдиальной стороны ΡΒ Ρ†Π΅Π»ΡŒΡŽ выявлСния ΠΈΠ½Π΄ΠΈΠ³Π΅Π½Π½Ρ‹Ρ… ΡˆΡ‚Π°ΠΌΠΌΠΎΠ² молочнокислых ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ², способных ΠΏΡ€ΠΎΠ΄ΡƒΡ†ΠΈΡ€ΠΎΠ²Π°Ρ‚ΡŒ Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΎΡ†ΠΈΠ½Ρ‹. Из 36Β ΡˆΡ‚Π°ΠΌΠΌΠΎΠ² ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ², Π²Ρ‹Π΄Π΅Π»Π΅Π½Π½Ρ‹Ρ… ΠΈΠ· смывов, ΠΎΡ‚ΠΎΠ±Ρ€Π°Π½Π½Ρ‹Ρ… ΡΒ Ρ‚ΡƒΡˆ, Ρ‚ΠΎΠ»ΡŒΠΊΠΎ 14 Π±Ρ‹Π»ΠΈ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΠΎΠ²Π°Π½Ρ‹ для дальнСйшСго исслСдования ΠΈΒ ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Ρ‹ ΠΊΠ°ΠΊ молочнокислыС Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΈ (ΠœΠšΠ‘). ΠŸΡ€ΠΈ ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΠΈ ΠΈΡ… бактСриоциногСнности ΠΏΠΎ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠ΅ двухслойных Π°Π³Π°Ρ€ΠΎΠ² ΠΌΠΎΠ΄ΠΈΡ„ΠΈΡ†ΠΈΡ€ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ состава, Π±Ρ‹Π»Π° Π΄ΠΎΠΊΠ°Π·Π°Π½Π° высокая ΠΈΠ½Π³ΠΈΠ±ΠΈΡ€ΡƒΡŽΡ‰Π°Ρ ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Π²Β ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠΈ роста ΠΏΠ°Ρ‚ΠΎΠ³Π΅Π½Π½ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠ° Salmonella typhimurium, Ρ‚ΠΎΠ»ΡŒΠΊΠΎ 6 Π²Ρ‹Π΄Π΅Π»Π΅Π½Π½Ρ‹Ρ… ΡˆΡ‚Π°ΠΌΠΌΠΎΠ² молочнокислых Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ. ИспользованиС Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Ρ‚ΠΎΠ΄Π° ΠΎΡ†Π΅Π½ΠΊΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ Π½Π΅ Ρ‚ΠΎΠ»ΡŒΠΊΠΎ Π²Ρ‹ΡΠ²ΠΈΡ‚ΡŒ Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ способности ΠœΠšΠ‘ ΠΏΡ€ΠΎΠ΄ΡƒΡ†ΠΈΡ€ΠΎΠ²Π°Ρ‚ΡŒ Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΎΡ†ΠΈΠ½Ρ‹, Π½ΠΎ ΠΈΒ ΠΈΠ·ΡƒΡ‡ΠΈΡ‚ΡŒ ΠΈΡ… Π°Π½Ρ‚Π°Π³ΠΎΠ½ΠΈΡΡ‚ΠΈΡ‡Π΅ΡΠΊΡƒΡŽ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Π²Β ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΈΡ… ΠΈΠ·ΡƒΡ‡Π°Π΅ΠΌΡ‹Ρ… ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ², ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΡΠ²Π»ΡΡŽΡ‚ΡΡ ΠΈΠ½Π΄ΠΈΠΊΠ°Ρ‚ΠΎΡ€Π°ΠΌΠΈ соблюдСния Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… Ρ€Π΅ΠΆΠΈΠΌΠΎΠ² тСхнологичСских процСссов. НаличиС Ρ‚Π°ΠΊΠΈΡ… ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ² в мясС ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ ΡƒΠ²Π΅Π»ΠΈΡ‡ΠΈΡ‚ΡŒ срок хранСния мяса, Π·Π° счСт подавлСния роста близкородствСнных Π»Π°ΠΊΡ‚ΠΎΠ±Π°Ρ†ΠΈΠ»Π», Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΈΠ· ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… ΡΠ²Π»ΡΡŽΡ‚ΡΡ ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ ΠΏΠΎΡ€Ρ‡ΠΈ, Π°Β Ρ‚Π°ΠΊΠΆΠ΅ рост условно-ΠΏΠ°Ρ‚ΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΈΒ ΠΏΠ°Ρ‚ΠΎΠ³Π΅Π½Π½ΠΎΠΉ ΠΌΠΈΠΊΡ€ΠΎΡ„Π»ΠΎΡ€Ρ‹. ΠšΡ€ΠΎΠΌΠ΅ этого, ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ молочнокислых Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ, ΠΎΠ±Π»Π°Π΄Π°ΡŽΡ‰ΠΈΡ… Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΎΡ†ΠΈΠ½ΠΎΠ³Π΅Π½Π½ΠΎΡΡ‚ΡŒΡŽ, Π²Β Ρ‚Π΅Ρ…Π½ΠΎΠ»ΠΎΠ³ΠΈΠΈ производства сырокопчСных колбас ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ‚ ΡΠ½ΠΈΠ·ΠΈΡ‚ΡŒ риск производства нСбСзопасной ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ, Π½Π°Π»ΠΈΡ‡ΠΈΠ΅ в мясС ΠΈΠ½Π΄ΠΈΠ³Π΅Π½Π½Ρ‹Ρ… ΠœΠšΠ‘ являСтся Π΄ΠΎΠΏΠΎΠ»Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΌ Ρ„Π°ΠΊΡ‚ΠΎΡ€ΠΎΠΌ, ΠΎΠ±Π΅ΡΠΏΠ΅Ρ‡ΠΈΠ²Π°ΡŽΡ‰ΠΈΠΌ ΠΏΡ€ΠΎΡ‚Π΅ΠΊΠ°Π½ΠΈΠ΅ ΠΆΠ΅Π»Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… биохимичСских процСссов ΠΈΒ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½ΠΎΡΡ‚ΡŒ сырокопчСных колбас.ΠšΡ€ΠΈΡ‚Π΅Ρ€ΠΈΠΈ Π°Π²Ρ‚ΠΎΡ€ΡΡ‚Π²Π°ΠžΡ‚Π²Π΅Ρ‚ΡΡ‚Π²Π΅Π½Π½ΠΎΡΡ‚ΡŒ Π·Π° Ρ€Π°Π±ΠΎΡ‚Ρƒ ΠΈΒ  прСдоставлСнныС свСдСния нСсут всС Π°Π²Ρ‚ΠΎΡ€Ρ‹. ВсС Π°Π²Ρ‚ΠΎΡ€Ρ‹ Π²Β Ρ€Π°Π²Π½ΠΎΠΉ стСпСни участвовали в этой Ρ€Π°Π±ΠΎΡ‚Π΅. Π‘Π°Ρ‚Π°Π΅Π²Π° Π”.Π‘. Ρ€Π°Π·Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°Π»Π° Π½Π°ΡƒΡ‡Π½ΠΎ-мСтодичСскиС ΠΏΠΎΠ΄Ρ…ΠΎΠ΄Ρ‹ ΠΊΒ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡŽ Ρ€Π°Π±ΠΎΡ‚, опрСдСляла объСм исслСдований, Π°Π½Π°Π»ΠΈΠ·ΠΈΡ€ΠΎΠ²Π°Π»Π° ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π½Ρ‹Π΅ Π΄Π°Π½Π½Ρ‹Π΅, выполняла ΠΎΠΏΠΈΡΠ°Ρ‚Π΅Π»ΡŒΠ½ΡƒΡŽ Ρ‡Π°ΡΡ‚ΡŒ ΡΡ‚Π°Ρ‚ΡŒΠΈ ΠΈΒ ΠΊΠΎΡ€Ρ€Π΅ΠΊΡ‚ΠΈΡ€ΠΎΠ²Π°Π»Π° послС ΠΏΠΎΠ΄Π°Ρ‡ΠΈ Π²Β Ρ€Π΅Π΄Π°ΠΊΡ†ΠΈΡŽ Π‘ΠΎΠΊΠΎΠ»ΠΎΠ²Π° О.Π’. ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»Π° ΠΎΠ±Π·ΠΎΡ€ ΠΈΒ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΡ‚Π΅Ρ€Π°Ρ‚ΡƒΡ€Ρ‹, выполняла ΠΎΠΏΠΈΡΠ°Ρ‚Π΅Π»ΡŒΠ½ΡƒΡŽ Ρ‡Π°ΡΡ‚ΡŒ Π—Π°ΠΉΠΊΠΎ Π•.Π’. ΠΎΡ‚Π±ΠΈΡ€Π°Π»Π° ΠΎΠ±ΡŠΠ΅ΠΊΡ‚Ρ‹ исслСдования, выполняла микробиологичСский Π°Π½Π°Π»ΠΈΠ·. Пашкова Π’.Π’. выполняла ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ Ρ€Π°Π±ΠΎΡ‚Ρ‹, микробиологичСскиС исслСдования. Авторы Π²Β Ρ€Π°Π²Π½Ρ‹Ρ… долях ΠΈΠΌΠ΅ΡŽΡ‚ ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠ΅ к написанию рукописи ΠΈΒ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΠΎ нСсут ΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²Π΅Π½Π½ΠΎΡΡ‚ΡŒ Π·Π° ΠΏΠ»Π°Π³ΠΈΠ°Ρ‚.ΠšΠΎΠ½Ρ„Π»ΠΈΠΊΡ‚ интСрСсовАвторы Π·Π°ΡΠ²Π»ΡΡŽΡ‚ ΠΎΠ± отсутствии ΠΊΠΎΠ½Ρ„Π»ΠΈΠΊΡ‚Π° интСрСсов

    ASSESSMENT OF THE BACTERIOCINOGENICITY OF INDIGEN LACTOBACILLUS ONTO CATTLE CARCASSES

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    Cattle carcasses of ritual slaughter (Halal) from the lateral and medial side were investigated in order to identify the indigenous strains of lactic acid microorganisms capable of producing bacteriocins. Only 14 strains, from 36 strains of microorganisms isolated from washings taken from carcasses, were used for further research and identified as lactabacilus. In the study of their bacteriocinogenicity by the method of two-layer agars of modified composition, high inhibitory efficiency was proved with respect to the growth of the pathogen Salmonella typhimurium, only 6 isolated strains of lactabacilus. The use of this method of evaluation will not only reveal the presence of the ability of the lactabacilus to produce bacteriocins, but also to study their antagonistic activity against many of the studied microorganisms, which are indicators of compliance with various modes of technological processes. The presence of such microorganisms in the meat will increase the shelf life of meat, due to the suppression of the growth of closely related lactabacilus, some of which are spoilage microorganisms, as well as the growth of opportunistic and pathogenic microflora. In addition, the use of lactabacilus with bacteriocinogenicity in the production technology of raw sausages will reduce the risk of production of unsafe products. Thus, the presence of indigenous lactabacilus in meat is an additional factor that ensures the flow of desired biochemical processes and the safety of raw smoked sausages.ContributionAll authors bear responsibility for the work and presented data. All authors made an equal contribution to the work. Dagmara S. Bataeva developed scientific and methodological approaches to work, determined the scope of research, analyzed the data obtained, performed the narrative and corrected it after submitting to the editorial office. Olga V. Sokolova conducted a review and analysis of the literature, carried out the descriptive part Elena V. Zaiko selected research objects, carried out microbiological analysis. Victoria V. Pashkova carried out preparatory work and microbiological analysis. The authors were equally involved in writing the manuscript and bear the equal responsibility for plagiarism.Conflict of interestThe authors declare no conflict of interest
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