Synthesis of 2-substituted 8-propargyloxyquinoline derivatives and determination of their antioxidant, antibacterial and DNA binding activities

One-pot synthesis of novel quinoline- and quinolone-substituted 1,2,3-triazoles has been performed from the key intermediates, quinoline- and quinolone-substituted propargyl derivatives 1–3 (48%–88% yields). The antioxidant properties of the newly synthesized compounds, 1a–1c, 2a–2c, and 3a–3c, were evaluated by monitoring DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging abilities, metal chelating effects, and reducing power. The scavenging effects of compounds on the free radical decreased in the order of 3b > 3a > 1b and were found to be 36.3%, 34.9%, and 27.6% at the concentration of 500 g/mL, respectively. All of the compounds showed low chelating capacity. Furthermore, the antibacterial activity was studied against gram-positive and gram-negative bacteria and the DNA binding ability of the compounds was evaluated with calf thymus DNA using agarose gel electrophoresis

Heavy metals biosorption by submerged aquatic plant nasturtium officinale

In the present investigation an aquatic plant, Nasturtium officinale was examined for its biosorption ability of heavy metal ions. The results obtained from biosorption experiments were used to understand the driving forces that govern the interaction between metal ions and biosorbent. The experimental biosorption data were fitted to the Scatchard plot, Langmiur and Freundlich isotherms. According to the parameters of the Langmiur isotherm, the maximum biosorption capacities of Cd2+ and Co2+ were 0.56 and 0.49 mg/g, respectively. In view of Freundlich isotherms, the maximum biosorption capacities of Cd2+ and Co2+ were 2.23 mg/g for both of metal ions

İmmobilize Coriolus versicolor kullanılarak Pb (II) iyonlarının biyosorpsiyonu ve önderiştirilmesi

Ağır metaller gıda sektörünün başlıca kirleticileri arasında yer alır ve özellikle bizim gibi gelişmekte olan ülkelerde en önemli sorunlardan biri olarak düşünülebilir. Gıdalardaki ağır metallerin aşırı içeriği, özellikle kardiyovasküler, böbrek, sinir ve diğer hastalıklarla birlikte bir dizi hastalığın etiyolojisi ile ilişkilidir. Kurşun, ciddi bir kümülatif vücut zehiridir ve vücut sistemimize hava, su ve yiyecek yoluyla girer. Bazı ülkelerde insanlar, içme suyunun kurşun borulardan kaynaklanan kirlenmeleri nedeniyle önemli ölçüde bu metale maruz kalabilir. Kurşun kirliliğinin çoğu kümülatif etki gösterir ve insanlar kurşun ile uzun süre teması sonucunda, küçük konsantrasyonlarda bile sağlığa zararlı olabilir. Gıda ve su örneklerinde kurşun gibi metal iyonlarının aletli analizle doğrudan belirlenmesi, düşük miktarda olmaları nedeniyle çoğu zaman zordur. Bu nedenle, kantitatif olarak belirlenmeden önce genellikle bir ayırma ve önderiştirme tekniği gereklidir. Bu çalışmada Katı faz ekstraksiyonu (SPE) yöntemi ile Pb iyonlarının önderiştirilmesi için alternatif bir yöntem geliştirilmiştir., Pb'nin indüktif eşleşmiş plazma optik emisyon spektrometrisi (ICP-OES) ile belirlenmesinden önce önderiştirilmesi için immobilize Coriolus versicolor fungal biyosorbent olarak kullanıldı. Geliştirilen SPE yöntemindeki bazı parametreler (pH, akış hızı, biyosorbent miktarı, kolonun tekrar kullanılabilirliği) optimize edildi. Yöntemin doğruluğu sertifikalı referans çay numunelerinin analizi ile doğrulandı. Geliştirilen kolonun 30 kullanımdan sonra bile oldukça stabil olduğu belirlendi

Synthesis of 2-substituted 8-propargyloxyquinoline derivatives and determination of their antioxidant, antibacterial and DNA binding activities. Turkish Journal of Chemistry

2-Substituted 8-propargyloxyquinoline derivatives have been synthesized from 2-substituted-8-hydroxyquinolines by O-propargylation method (30%–98% yields). The newly synthesized compounds were tested for in vitro antioxidant activities such as DPPH radical scavenging, ferrous chelating, and reducing power activities. The maximum radical scavenging (46.5%) and reducing power activities were obtained from 1 and maximum ferrous chelating effect was obtained from 6 (72.1%) at the concentration of 500 g/mL. To indicate DNA binding activity of the complexes calf thymus DNA was used. The compounds were also evaluated for their antimicrobial activity against three gram-positive and three gram-negative bacteria. Compounds 3 and 5 exhibited antibacterial activity against all tested gram-positive bacteria

The use of fungal biomass agaricus bisporus immobilized on amberlite XAD-4 resin for the solid phase preconcentration of thorium

Solid-phase extraction method was developed for the preconcentration of thorium (Th). Fungal biomass Agaricus bisporus was immobilized to Amberlite XAD-4 as solid-phase sorbent. The critical parameters such as pH of the sample solution, flow rate of the sample, volume of the sample, and the effect of major ions that affect the preconcentration of thorium in this system were evaluated. The optimum pH for the sorption of Th is 6.0, and quantitative elution occurs with 1.0 mol L−1 HCl. The loading capacity was determined as 0.079 mmol g−1. The optimized method was validated through analysis of the certified reference material of tea leaves (NCS ZC73014) and successfully applied to the determination of Th in a real ore sample with satisfactory results

DNA cleavage properties and synthesis of metallophthalocyanines with 5-methyl-[1, 2, 4] triazolo [1, 5-a] pyrimidin-7-oxy substituents.

The aim of the present study is to perform synthesis of novel metallophthalocyanines (pcs) with high solubility. The synthesis and characterization of 5-methyl-[1, 2, 4] triazolo [1, 5-a] pyrimidin-7-yloxysubstituted zinc, magnesium, and cobalt pcs are reported. These compounds have been characterized using electronic absorption, nuclear magnetic resonance spectroscopy, infrared, elemental analysis and mass spectra. The aggregation investigations carried out in different concentrations indicate that 5- methyl-[1, 2, 4] triazolo [1, 5-a] pyrimidin-7-yloxy-substituted pc complexes do not have any aggregation behavior for the concentration range of 1 £ 10¡5¡1 £ 10¡6 M in THF. DPPH radical scavenging activity, metal chelating activity, and reducing power of the compounds were evaluated. The DNA gel electrophoresis studies revealed that new phthalonitrile and its metallophthalocyanine compounds cleavaged plasmid DNA (pBR322). Additionally, the ground-state geometries of the complexes were optimized using density functional theory methods at B3LYP/6-31G (d, p) level in order to obtain information about the 3D geometries and electronic structure

Synthesis of some novel phthalocyanines with methyl 2-(oxy)-2, 2-diphenylacetate substituents, evaluation of their antioxidant- antibacterial activities and electronic properties

The synthesis, characterization, spectral, antioxidant, and antibacterial properties of methyl 2-(oxy)-2,2-diphenylacetate substituted metallophthalocyanines (4–7) are reported. A novel phthalonitrile, methyl 2-(2,3-dicyanophenoxy)-2,2-diphenylacetate (3) was prepared and characterized. The new compounds were characterized by using electronic absorption spectroscopy, nuclear magnetic resonance spectroscopy, infrared spectroscopy, and elemental analysis. All compounds were tested for their antioxidant activity by using 2,2- diphenyl-1-picrylhydrazyl radical scavenging ability, metal chelating, and reducing power. Furthermore, these compounds were screened for their in vitro growth inhibiting activity by disc diffusion method against four gram positive and two gram negative bacteria. The electronic data of the new compounds were obtained by computational calculations at B3LYP/6-31G(d,p) level of theory

Simultaneous removal of indomethacine, papaverine and allopurinol from aqueous solution by using submerged aquatic plant nasturtium officinale

Simultaneous removal of indomethacine, papaverine and allopurinol from aqueous solution by using submerged aquatic plant Nasturtium officinale biomass in high performance thin layer chromatography (HPTLC) was studied. Optimum biosorption conditions were determined as a function of contact time, pH, removal capacity of the amount of biomass and initial dry concentration. Experiments were performed in batch conditions. Concentrations of the drugs in the remaining solutions were simultaneously analyzed by HPTLC. Langmuir and Freundlich models were applied to describe the biosorption isotherm of the drugs by aquatic plant Nasturtium officinale biomass. According to the results, optimum parameters were found as 2.0 g biomass, pH:5.0 and 60 min contact time. Obtained from plots of Langmuir and Freundlich adsorption models, the highest drug uptakes were calculated from Langmuir isotherm and found to be 43.10, 39.68 and 38.61 mg/g for indomethacine, papaverine and allopurinol, respectively

Antioxidant properties of cultured mycelia from four pleurotus species produced in submerged medium.

The ethanolic extracts of dried cultured mycelia of Pleurotus ostreatus, Pleurotus eryngii, Pleurotus florida, and Pleurotus sajor-caju were analyzed for antioxidant activity in different systems. Tests used are as follows: reducing power, free radical scavenging, superoxide anion radical scavenging, total antioxidant activity, metal chelating activitiy, etc.; total phenolic content was determined. The percentage inhibition of P. ostreatus, P. eryngii, P. florida, and P. sajor-caju at 20 mg/mL concentration on peroxidation in a β-carotene–linoleic acid system was 57.19, 60.68, 62.12, and 58.81%, respectively. The reducing power of P. eryngii was higher than the other samples, and its value was 0.86 at 10 mg/mL concentration. P. ostreatus and P. sajor-caju proved to be better at scavenging superoxide anion radicals than the P. eryngii and P. florida. In the scavenging effect of DPPH radical test, P. ostreatus showed the highest activity potential and P. sajor-caju showed the strongest metal chelating capacity

Metallo phthalocyanines bearing (2-((2-hydroxyethyl) (p-tolyl)amino)ethoxy) and (2-((2-phenoxyethyl) (p-tolyl)amino)ethoxy) phthalonitrile substituents: Synthesis, characterization, aggregation behavior, antioxidant and antibacterial activity

tThe novel phthalonitrile derivatives bearing (2-((2-hydroxyethyl)(p-tolyl)amino)ethoxy) and (2-((2-phenoxyethyl)(p-tolyl)amino)ethoxy)phthalonitrile substituents at peripheral position were preparedby a nucleophilic displacement reaction. Cyclotetramerization of these phthalonitrile derivatives in thepresence of corresponding metal salts gave the new metallophthalocyanines. The novel compounds werecharacterized by using various spectroscopic data. The aggregation investigations carried out in differ-ent concentrations indicate that phthalocyanine compounds do not have any aggregation behavior forthe concentration range of 10−4–10−6M in tetrahydrofuran. In vitro three antioxidant test methods,namely diphenylpicrylhydrazyl radical scavenging activity, metal chelating activity and reducing powerwere used to determine the antioxidant activity of compounds. Compounds were analyzed for theirantibacterial activity against some bacteria by using the disk-diffusion method