7 research outputs found
Hydrolysis of Crystalline Cellulose by Cellobiohydrolases Cel6a & Cel7a from Trichoderma reesei and their Synergistic Effects
Genetic variation of the 5âHT1A rs6295, 5âHT2A rs6311, and CNR1 rs1049353 and an altered endocannabinoid system in depressed patients
Abstract Background The reasons for developing depression are not fully understood. However, it is known that the serotonergic system plays a role in the etiology, but the endocannabinoid system receives attention. Method In this study, 161 patients with a depressive disorder and 161 healthy participants were examined for the distribution of the CNR1 rs4940353, 5âHT2A rs6311, and 5âHT1A rs6295 by highâresolution melting genotyping. The concentration of arachidonoyl ethanolamide (AEA) and 2âarachidonoylglycerol (2âAG) in the blood was measured by liquid chromatographyâtandem mass spectrometry. Additionally, depression and anxiety symptoms were evaluated based on selfâquestionnaires. Fiftyânine patients participated in a second appointment to measure the concentration of AEA, 2âAG, and symptoms of depression and anxiety. Results We observed higher AEA and decreased 2âAG concentrations in patients with depression compared to healthy participants. During the treatment, the concentrations of AEA and 2âAG did not change significantly. In patients higher symptoms of anxiety correlated with lower concentrations of 2âAG. Gender differences were found concerning increased 2âAG concentration in male patients and increased anxiety symptoms in female patients. Genotypic variations of 5âHT1A rs6295 and 5âHT2A rs6311 are associated with altered serotonergic activity and serotonin content in patients. Conclusion In conclusion, it seems that the endocannabinoid system, especially the endocannabinoids 2âAG and AEA, and genetic variations of the 5âHT1A and 5âHT2A could play a role in patients with depression and may be involved in a depressive disorder
Genetic variation of the 5-HT1A rs6295, 5-HT2A rs6311, and CNR1 rs1049353 and an altered endocannabinoid system in depressed patients
The reasons for developing depression are not fully understood. However, it is known that the serotonergic system plays a role in the etiology, but the endocannabinoid system receives attention.
In this study, 161 patients with a depressive disorder and 161 healthy participants were examined for the distribution of the CNR1 rs4940353, 5-HT2A rs6311, and 5-HT1A rs6295 by high-resolution melting genotyping. The concentration of arachidonoyl ethanolamide (AEA) and 2-arachidonoylglycerol (2-AG) in the blood was measured by liquid chromatographyâtandem mass spectrometry. Additionally, depression and anxiety symptoms were evaluated based on self-questionnaires. Fifty-nine patients participated in a second appointment to measure the concentration of AEA, 2-AG, and symptoms of depression and anxiety.
We observed higher AEA and decreased 2-AG concentrations in patients with depression compared to healthy participants. During the treatment, the concentrations of AEA and 2-AG did not change significantly. In patients higher symptoms of anxiety correlated with lower concentrations of 2-AG. Gender differences were found concerning increased 2-AG concentration in male patients and increased anxiety symptoms in female patients. Genotypic variations of 5-HT1A rs6295 and 5-HT2A rs6311 are associated with altered serotonergic activity and serotonin content in patients.
In conclusion, it seems that the endocannabinoid system, especially the endocannabinoids 2-AG and AEA, and genetic variations of the 5-HT1A and 5-HT2A could play a role in patients with depression and may be involved in a depressive disorder