Farrowing accommodation for organic pigs

Newborn piglets in organic farrowing pens have a lower survival rate than in conventional farrowing pens. This difference is mainly caused by housing the sow loose compared to crated and by climatic effects of the outdoor temperature. Organic lactating sows should have at least 7.5 m² indoor area with straw and a 2.5 m² outdoor run. The aim of the project was to increase piglet survival in order to improve animal welfare as well as the profitability of organic farms. In the experiment we compared survival and behaviour in 3 pen types: type 1 with outdoor run, type 2 with an indoor run and a higher proportion of solid floor and type 3 without run. Data was analysed with Analysis of Variance using parity and liveborn piglets as covariables. Results of 131 litters in total showed 9.6a, 10.8b and 9.4a (p=0.05) weaned piglets per litter for pen type 1, 2 and 3. Fouling scores indicating dunging behaviour in the indoor lying area showed 13a, 21b and 19b (p=0.04) for pen types 1, 2 and 3. We found a tendency that litters with high survival rates used the separate piglet nest sooner for lying than the litters with low survival rates. Climatic conditions seemed to be crucial for the vitality and survival of the newborn piglets. The better climatic conditions combined with the higher proportion of solid floor resulted in a higher survival rate of the piglets. These results are currently used in a second experiment focussing on extra heating around farrowing and solid floor proportion in a new farrowing accommodation

Effect of cleaning /disinfection strategies on helminth infections in finishing pigs

Ascaris suum is the most prevalent helminth on organic pig farms (Carstensen et al., 2002) and is transmitted mainly via the faeces. The use of anthelmintics does not fit in the organic principles and preventive measures are promoted. This project focused on assessing the efficacy of a cleaning protocol for the dunging area of pens on Ascaris s. transmission to pigs. In 4 batches with 8 identical pens for 15 pigs each (n=480 pigs) 6 pigs per pen were orally infected with Ascaris s. The other pigs can be earliest infected at 10 weeks and half of the pens were thoroughly cleaned at that time. Affected livers and egg counts in the manure had to show if this cleaning protocol keeps the non infected animals free of Ascaris s. The results show no effect of the cleaning protocol on the non infected pigs. These pigs had 57% damaged livers in both treatment and 50% had positive egg counts in the manure. The conclusion is that pen hygiene does not contribute to a reduction of Ascaris s. infections

Effect of rooting area and drinker in the outdoor run on behaviour and Ascaris infection of organic pigs

Hygiene is often a problem on the outdoor runs of growing organic pigs. Manure and urine are mainly excreted outside, but spread all over the run. Reduc-ing the dirty surface may improve well being, reduce ammonia emission, improve hygiene and reduce labour and parasite infections. The presence of a rooting area and of a drinker on the outdoor run were tested in a 2x2 factorial design. The pens with the rooting areas had a higher number of pigs on the outdoor run than the pens without a rooting area (1.6 vs 1.2 pigs). This was caused by more pigs in area 2 and 3. At higher temperatures there were more pigs outside, except in the root-ing area: this was popular at all temperatures. A rooting area resulted in a cleaner outdoor area, however in some cases the root-ing area became a dunging area. An extra outdoor drinker leads to a cleaner area around the drinker, but to a dirtier indoor area. No difference in Ascaris infection was found between the four treatment combinations

Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis

Human papillomavirus induced (HPV+) cancer incidence is rapidly rising, comprising 60–80% of oropharyngeal squamous cell carcinomas (OPSCCs); while rare, recurrent/metastatic disease accounts for nearly all related deaths. An in vivo pre-clinical model for these invasive cancers is necessary for testing new therapies. We characterize an immune competent recurrent/metastatic HPV+ murine model of OPSSC which consists of four lung metastatic (MLM) cell lines isolated from an animal with HPV+ OPSCC that failed cisplatin/radiation treatment. These individual metastatic clonal cell lines were tested to verify their origin (parental transgene expression and define their physiological properties: proliferation, metastatic potential, heterogeneity and sensitivity/resistance to cisplatin and radiation. All MLMs retain expression of parental HPV16 E6 and E7 and degrade P53 yet are heterogeneous from one another and from the parental cell line as defined by Illumina expression microarray. Consistent with this, reverse phase protein array defines differences in protein expression/activation between MLMs as well as the parental line. While in vitro growth rates of MLMs are slower than the parental line, in vivo growth of MLM clones is greatly enhanced. Moreover, in vivo resistance to standard therapies is dramatically increased in 3 of the 4 MLMs. Lymphatic and/or lung metastasis occurs 100% of the time in one MLM line. This recurrent/metastatic model of HPV+ OPSCC retains the characteristics evident in refractory human disease (heterogeneity, resistance to therapy, metastasis in lymph nodes/lungs) thus serving as an ideal translational system to test novel therapeutics. Moreover, this system may provide insights into the molecular mechanisms of metastasis

Prevention and treatment of parasitic infections in organic pigs

Organic and free range pigs are potentially exposed to a wider range of parasites and zoonotic challenges. The livestock Subproject QLIF addresses 4 strategies to combat these challenges, and the present paper describes the initial results. Rodent control is the first strategy, and a survey concludes that Salmonella and Campylobacter infections were encountered in house mice and Norway rats, but not in other species. T. gondii antibodies could be detected in 6.4% of the blood samples taken from 235 wild small mammals. To reduce the presence of rodents around farms, the use of live-traps may be a good alternative for the use of rodenticides on organic farms. A second strategy is aimed at directing pig dunging behaviour in such a way that contact with (infected) faeces is minimised. The study showed that a rooting area resulted in a cleaner outdoor are and an extra outdoor drinker led to a cleaner area around the drinker, but to a dirtier indoor area. However, no difference in Ascaris suum infection were found. The nutritional strategy tested showed that pure inulin appeared the most effective as Oesophagostomum dentatum presence was reduced by 91%. Finally, as part of Strategy 4, a mixture of dried Thymus vulgaris, Melissa officinalis and Echinacea purpurea in a dosage of 5% in the diet showed to be preventive against a mild round-worm infection. The same mixture, however, was not effective in a lower dosage (3% in the diet) against a serious round-worm infection. Work is continuing to develop the above strategies further

Portion size: review and framework for interventions

The prevalence of overweight and obesity has increased. A strong environmental factor contributing to the obesity epidemic is food portion size. This review of studies into the effects of portion size on energy intake shows that increased food portion sizes lead to increased energy intake levels. Important mechanisms explaining why larger portions are attractive and lead to higher intake levels are value for money and portion distortion. This review also shows that few intervention studies aiming to reverse the negative influence of portion size have been conducted thus far, and the ones that have been conducted show mixed effects. More intervention studies targeted at portion size are urgently needed. Opportunities for further interventions are identified and a framework for portion size interventions is proposed. Opportunities for intervention include those targeted at the individual as well as those targeted at the physical, economic, political and socio-cultural environment

Dynamic cytokinin signalling landscapes during lateral root formation in Arabidopsis

By forming lateral roots, plants expand their root systems to improve anchorage and absorb more water and nutrients from the soil. Each phase of this developmental process in Arabidopsis is tightly regulated by dynamic and continuous signalling of the phytohormones cytokinin and auxin. While the roles of auxin in lateral root organogenesis and spatial accommodation by overlying cell layers have been well studied, insights on the importance of cytokinin is still somewhat limited. Cytokinin is a negative regulator of lateral root formation with versatile modes of action being activated at different root developmental zones. Here, we review the latest progress made towards our understanding of these spatially separated mechanisms of cytokinin-mediated signalling that shape lateral root initiation, outgrowth and emergence and highlight some of the enticing open questions

Extra snijmais goed voor welzijn varkens

Vleesvarkens hebben graag een emmer snijmais. Dat blijkt uit praktijkonderzoek op Varkens Innovatie Centrum Sterksel, onderdeel van Wageningen UR. In vergelijking met stro heeft snijmais meer voederwaarde en het leidt minder snel tot verstoppingen in de mestput