The Lantern Vol. 29, No. 1, December 1961

• King Fitzgerald\u27s Court • Deliberate Beyond Conception • Jean • The Dumb Superman • Epilogue to Death • Afternoon in August • Eichmann • A Nose Thumbed • Observation • The Forgetful Evening • Truditur Dies Die • The Deserted Pier • Thoughts on Love • Laughter • A Waste? • Villanelle: Interlude • Lines on a Rhetorical Question • Villanellehttps://digitalcommons.ursinus.edu/lantern/1081/thumbnail.jp

The Lantern Vol. 29, No. 2, Spring 1962

• A Deadly Diatribe on Daydreaming • Iter Animae • In Retrospection • Collegiate, Collegiate, Yes We Are Collegiate • Saint Zachary • Lost Horizons • Rune Green Stones • Druidics • Leanthalamion • Thoughts on Leaving Derr Hall • Opus 36; Literature 3 • Chinese Gill • Times of Sandhttps://digitalcommons.ursinus.edu/lantern/1082/thumbnail.jp

The Lantern Vol. 30, No. 1, February 1963

• Mechanical Duplicity • The Practical Profits of Purism • \u27Tis Better • Misha • Manuel • An American Fairy Tale • The Christ of Christopher Street • Nocturne • Various Reflections • He Came and Gently Lifted Me • Poem, In a Minor Key • World Fell to Ruin • The Map • On Being Jilted • Manna • Traitor • The Leaves Cling • Oh Freedom! • The Insurance Man • Translation - The Vampire • Four Poems • Sanguis • Phoney is the Color of My Love\u27s Life • To a Barmaidhttps://digitalcommons.ursinus.edu/lantern/1083/thumbnail.jp

The Lantern Vol. 32, No. 1, January 1965

• The Wise Man • Of Men and Lobsters • Deliberate-Beyond Conception • Villanelle • Villanelle: Interlude • Rune Green Stones • Redemption • John Ten • Torch Ends • The General and the Birdnest • Not Quite Free • Hymn to the Morning • Walking Togetherhttps://digitalcommons.ursinus.edu/lantern/1087/thumbnail.jp

The Lantern Vol. 30, No. 2, May 1963

• An Observation • Interim • The Collected Raid • Please • Love Me • Waiting With the Sun • Upon a Summit • A Hedonist Comments on His Religion • Like Ice • Dark Morning • So Soft the Breeze • The Heat of Youth • Solemnity • A Laugh • Peter Departinghttps://digitalcommons.ursinus.edu/lantern/1084/thumbnail.jp

The Lantern Vol. 28, No. 2, Spring 1961

• A New Bedlam • A Priori • Germ Warfare • Verse for a Sympathy Card • On Lamartine\u27s Crucifix • On Art • Hope • Hymn to the Morning • An Educator Speaks • Come Out • Insemination • A Day\u27s Hope • Laura • Walking Together • 20 September 1960 • 15 October 1960 • The Governor\u27s Dog • One of the Gang • Poem • Knowledge is Freedom • To Conservative Child • Seventeen American Skating Careers at the Zenithhttps://digitalcommons.ursinus.edu/lantern/1080/thumbnail.jp

Spike Protein Fusion Peptide and Feline Coronavirus Virulence

Mutations can occur erratically and accompany tropism changes, resulting in unpredictable new diseases

Pathogenic characteristics of persistent feline enteric coronavirus infection in cats

Feline coronaviruses (FCoV) comprise two biotypes: feline enteric coronaviruses (FECV) and feline infectious peritonitis viruses (FIPV). FECV is associated with asymptomatic persistent enteric infections, while FIPV causes feline infectious peritonitis (FIP), a usually fatal systemic disease in domestic cats and some wild Felidae. FIPV arises from FECV by mutation. FCoV also occur in two serotypes, I and II, of which the serotype I viruses are by far the most prevalent in the field. Yet, most of our knowledge about FCoV infections relates to serotype II viruses, particularly about the FIPV, mainly because type I viruses grow poorly in cell culture. Hence, the aim of the present work was the detailed study of the epidemiologically most relevant viruses, the avirulent serotype I viruses. Kittens were inoculated oronasally with different doses of two independent FECV field strains, UCD and RM. Persistent infection could be reproducibly established. The patterns of clinical symptoms, faecal virus shedding and seroconversion were monitored for up to 10 weeks revealing subtle but reproducible differences between the two viruses. Faecal virus, i.e. genomic RNA, was detected during persistent FECV infection only in the large intestine, downstream of the appendix, and could occasionally be observed also in the blood. The implications of our results, particularly our insights into the persistently infected state, are discussed

Assembly of the Coronavirus Envelope: Homotypic Interactions between the M Proteins

The viral membrane proteins M and E are the minimal requirements for the budding of coronavirus particles. Since the E protein occurs in particles only in trace amounts, the lateral interactions between the M proteins apparently generate the major driving force for envelope formation. By using coimmunoprecipitation and envelope incorporation assays, we provide extensive evidence for the existence of such M-M interactions. In addition, we determined which domains of the M protein are involved in this homotypic association, using a mutagenetic approach. Mutant M proteins which were not able to assemble into viruslike particles (VLPs) by themselves (C. A. M. de Haan, L. Kuo, P. S. Masters, H. Vennema, and P. J. M. Rottier, J. Virol. 72:6838–6850, 1998) were tested for the ability to associate with other M proteins and to be rescued into VLPs formed by assembly-competent M proteins. We found that M proteins lacking parts of the transmembrane cluster, of the amphipathic domain, or of the hydrophilic carboxy-terminal tail, or M proteins that had their luminal domain replaced by heterologous ectodomains, were still able to associate with assembly-competent M proteins, resulting in their coincorporation into VLPs. Only a mutant M protein in which all three transmembrane domains had been replaced lost this ability. The results indicate that M protein molecules interact with each other through multiple contact sites, particularly at the transmembrane level. Finally, we tested the stringency with which membrane proteins are selected for incorporation into the coronavirus envelope by probing the coassembly of some foreign proteins. The observed efficient exclusion from budding of the vesicular stomatitis virus G protein and the equine arteritis virus M protein indicates that envelope assembly is indeed a highly selective sorting process. The low but detectable incorporation of CD8 molecules, however, demonstrated that this process is not perfect