О питьевой воде Украины

The criteria for assessing drinking water are considered. Nearly 40 years of author`s research involving 26 academic and departmental research institutes and laboratories in Ukraine and abroad, as well as 20-year medical clinical research and investigation by individual fellow scientists have shown the extremely high efficiency of the use of the silvaite mineral and its vitalized water in various sectors of the economy, science and technology. But they are of the greatest and primary interest for the production of healthy drinking water. Studies on the identification and research of the healing properties of drinking water are described.Рассмотрены критерии оценки питьевой воды. Почти 40-летние авторские исследования с привлечением 26 академических и ведомственных НИИ и лабораторий Украины и зарубежья, а также 20-летние медицинские клинические исследования и исследования отдельных коллег-ученых показали исключительно высокую эффективность использования минерала силевит и витализированной им воды в различных отраслях хозяйства, науки и техники. Но наибольший и первостепенный интерес представляют они для производства здоровой питьевой воды. Описаны исследования по выявлению и изучению оздоровительных свойств питьевой воды

Силевит и здоровая вода

Healthy water, besides meeting present requirements on structure, microbiological condition and other parameters, also pos­sesses ability to activate living organisms, plus has structurally-information adjustment for its self-improvement, allowing inde­pendently extinguish the centers of pathologies. Improvement of drinking water can be carried out uncompulsorily and naturally as it happens in the nature, by its activation by the natural mineral activator — silevit. The characteristics of waters activated by silevit and its improving action are stated. The implementation of a silevit makes possible the preparation of natural and steadily structured water with information saturation, i. e., healthy water.Здоровая питьевая вода, кроме соответствия традиционно определенным показателям, отвечает требованиям организма человека и обладает способностью активизировать его жизнедеятельность, наделяя его, благодаря своим энерго-информационным свойствам, настройкой на самооздоровление, самостоятельно гасить очаги патологий. Оздоровление воды осуществляется непосредственно, как это происходит в природе, путем ее активации природным минеральным активатором–силевитом. Приводится характеристика силевита, активированной (оздоровленной) им воды и ее оздоровительного воздействия. Отмечены направления и области эффективного практичного использования силевита и здоровой воды, прежде всего для оздоровления окружающей среды и населения, т. е. для преодоления экологического и демографического кризиса

Vitamin D Deficiency and Diabetes Mellitus

Vitamin D (VD) is a molecule that can be synthesized directly in the humans’ body or enter the organism with food in the form of inactive precursors. To exert its biological action, VD undergoes two-stage hydroxylation (at the 25th and 1st position) catalyzed by cytochromes P450, the presence of which has already been shown in almost all tissues of the human body. The product of hydroxylation is hormone-active form of vitamin D–1,25(OH)2D. 1,25(OH)2D binds to specific vitamin D receptor (VDR) and regulates the expression of genes involved in bone remodeling (classical function) and genes that control immune response, hormone secretion, cell proliferation, and differentiation (nonclassical functions). VD deficiency is prevalent around the globe and may be one of the key factors for diabetes development. The direct association between vitamin D deficiency and type 1 (T1D) and type 2 (T2D) diabetes has been proven. Detection of VDR in pancreas and adipose tissue, skeletal muscles, and immune cells allowed implying the antidiabetic role of vitamin D by enhancing insulin synthesis and exocytosis, increasing the expression of the insulin receptor, and modulating immune cells’ functions. This chapter summarizes data about relationship between VD insufficiency/deficiency and development of T1D and T2D, and their complications

Encapsulation of Viscous High-Fat Foods in Calcium Alginate Gel Tubes at Ambient Temperature

Viscous high-fat foods such as cream, egg yolk, or mayonnaise are co-extruded with a 3% sodium alginate solution from a syringe into a 50 mM calcium chloride solution. The food sample passes through the inner tube of a double needle assembly while the alginate solution is simultaneously extruded through a mantle surrounding the inner needle. As the sodium alginate solution forms a gel on contact with calcium ions, the food sample becomes encapsulated in the calcium alginate gel formed on the surface of the food sample. The encapsulation procedure may be carried out within a temperature range between 0 C and 25 C. Samples may be prepared for scanning electron microscopy or for transmission electron microscopy by selecting either wide or narrow bore needles, respectively

Endovascular Detection of Catheter-Thrombus Contact by Vacuum Excitation

Objective: The objective of this work is to introduce and demonstrate the effectiveness of a novel sensing modality for contact detection between an off-the-shelf aspiration catheter and a thrombus. Methods: A custom robotic actuator with a pressure sensor was used to generate an oscillatory vacuum excitation and sense the pressure inside the extracorporeal portion of the catheter. Vacuum pressure profiles and robotic motion data were used to train a support vector machine (SVM) classification model to detect contact between the aspiration catheter tip and a mock thrombus. Validation consisted of benchtop accuracy verification, as well as user study comparison to the current standard of angiographic presentation. Results: Benchtop accuracy of the sensing modality was shown to be 99.67%. The user study demonstrated statistically significant improvement in identifying catheter-thrombus contact compared to the current standard. The odds ratio of successful detection of clot contact was 2.86 (p=0.03) when using the proposed sensory method compared to without it. Conclusion: The results of this work indicate that the proposed sensing modality can offer intraoperative feedback to interventionalists that can improve their ability to detect contact between the distal tip of a catheter and a thrombus. Significance: By offering a relatively low-cost technology that affords off-the-shelf aspiration catheters as clot-detecting sensors, interventionalists can improve the first-pass effect of the mechanical thrombectomy procedure while reducing procedural times and mental burden

Pharmacological justification of the composition of active ingredients in the composition of «Molozol» gel

Today, in connection with the rapid increase in the number of skin diseases complicated by secondary microbial and fungal infection, the creation of new drugs with a complex dermatomycosis effect for their local use is gaining particular relevance. In modern dermatological practice, mostly soft dosage forms are used. The wide use of soft medicinal forms is due to the presence of some advantages: high local concentration in the skin, relative economy and manufacturability of these means. However, on the modern Ukrainian pharmaceutical market, the range of drugs with a complex dermatomycosis effect is limited. This makes it necessary to find new means for local application in dermatitis with complicated fungal pathology. The aim of our research is an experimental ground of rational composition of operating substances in a gael under the conditional name «Molozol» for treatment of dermatomycoses. Gel was prepared on general rules by preparations of soft medical forms. Taking into account physical and chemical properties of operating substances, in particular their solubility, sucking, salicylic and sorbic acid was entered as a solution in propylene glycol and entered in the basis procured in advance for gel. With the aim of ground of rational concentration milk, salicylic and sorbic acid in the combined gel of «Molozol», and also study of his biological activity were conducted microbiological and toxicological. Determination of the antibacterial properties of the gel was carried out by diffusion in agar using reference strains of microorganisms Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 885/653. The rational composition of the active substances of the gel under the conventional name «Molosol» for the treatment of dermatomycosis was experimentally substantiated. Based on the data of microbiological studies, the concentration of active substances – lactic acid, salicylic acid and sorbic acid in the composition of the gel «Molosol» was justified. With the combination of active substances salicylic acid 8%, lactic acid 10%, for sorbic acid concentration 0.5%, «Molosol» gel shows the most pronounced antimicrobial and fungicidal activity against the tested strains of microorganisms. The index of acute toxicity when applied to the skin of animals was determined, which proved that the developed soft drug «Molosol» in the form of a gel belongs to the IV class of low-toxic substances. The combined gel «Molosol» is promising for further research as an antimicrobial and antifungal agent with keratolytic properties

Vitamin D(3) regulates hepatic VEGF-A and apelin expression in experimental type 1 diabetes

The deficiency of vitamin D is associated with the risk of various chronic diseases, including diabetes mellitus and its complications. Given the strong genomic action of vitamin D hormone-active form, its deficiency can lead to dysfunction of cytokine signaling pathways, including those dependent on vascular endothelial growth factors (VEGFs) and apelin. The present study was carried out to define the link between VEGF-A and apelin expression in liver, hepatocytes viability and vitamin D status at experimental type 1 diabetes in mice. We established that chronic hyperglycemia at streptozotocin-induced diabetes was accompanied by a 2.2-fold decrease in 25OHD content in the serum and increased hepatocytes apoptosis and necrosis. Vitamin D deficiency correlated with increased apelin and VEGF-A (8- and 1.6-fold respectively) expression. Almost complete restoration of circulatory 25OHD content in serum was achieved at vitamin D3 treatment (800 IU/kg, per os, for 2 months) followed by reduced apelin and VEGF-A expression in liver and the decline of hepatocytes apoptosis. We conclude that vitamin D3 can be involved in cell survival, angiogenesis and fibrogenesis by modulating VEGF-A and apelin dependent regulatory systems in diabetic liver

Brain vitamin D3-auto/paracrine system in relation to structural, neurophysiological, and behavioral disturbances associated with glucocorticoid-induced neurotoxicity

IntroductionVitamin D3 (VD3) is a potent para/autocrine regulator and neurosteroid that can strongly influence nerve cell function and counteract the negative effects of glucocorticoid (GC) therapy. The aim of the study was to reveal the relationship between VD3 status and behavioral, structural-functional and molecular changes associated with GC-induced neurotoxicity.MethodsFemale Wistar rats received synthetic GC prednisolone (5 mg/kg b.w.) with or without VD3 (1000 IU/kg b.w.) for 30 days. Behavioral, histological, physiological, biochemical, molecular biological (RT-PCR, Western blotting) methods, and ELISA were used.Results and discussionThere was no difference in open field test (OFT), while forced swim test (FST) showed an increase in immobility time and a decrease in active behavior in prednisolone-treated rats, indicative of depressive changes. GC increased the perikaryon area, enlarged the size of the nuclei, and caused a slight reduction of cell density in CA1-CA3 hippocampal sections. We established a GC-induced decrease in the long-term potentiation (LTP) in CA1-CA3 hippocampal synapses, the amplitude of high K+-stimulated exocytosis, and the rate of Ca2+-dependent fusion of synaptic vesicles with synaptic plasma membranes. These changes were accompanied by an increase in nitration and poly(ADP)-ribosylation of cerebral proteins, suggesting the development of oxidative-nitrosative stress. Prednisolone upregulated the expression and phosphorylation of NF-κB p65 subunit at Ser311, whereas downregulating IκB. GC loading depleted the circulating pool of 25OHD3 in serum and CSF, elevated VDR mRNA and protein levels but had an inhibitory effect on CYP24A1 and VDBP expression. Vitamin D3 supplementation had an antidepressant-like effect, decreasing the immobility time and stimulating active behavior. VD3 caused a decrease in the size of the perikaryon and nucleus in CA1 hippocampal area. We found a recovery in depolarization-induced fusion of synaptic vesicles and long-term synaptic plasticity after VD3 treatment. VD3 diminished the intensity of oxidative-nitrosative stress, and suppressed the NF-κB activation. Its ameliorative effect on GC-induced neuroanatomical and behavioral abnormalities was accompanied by the 25OHD3 repletion and partial restoration of the VD3-auto/paracrine system.ConclusionGC-induced neurotoxicity and behavioral disturbances are associated with increased oxidative-nitrosative stress and impairments of VD3 metabolism. Thus, VD3 can be effective in preventing structural and functional abnormalities in the brain and behavior changes caused by long-term GC administration