HPLC analysis of phenolic compounds in leaves and inflorescences of

At least 22 phenolic compounds were identified in aqueous-ethanol extracts from Sorbaria pallasii (G. Don fil.) Pojark. leaves and at least 28 in its inflorescences by High-Performance Liquid Chromatography. Two acids (chlorogenic and p-hydroxybenzoic) and five flavonols (hyperoside, isoquercitrin, quercitrin, kaempferol, and astragalin) were identified among them. Flavonoid aglycones were obtained from the leaves and inflorescences by hydrochloric-acid hydrolysis of the aqueous-ethanol extracts (1:1). Three flavonol aglycones (quercetin, kaempferol, and isorhamnetin) were identified in the extract hydrolysates from S. pallasii above-ground organs through chromatographic analysis. Additionally, a flavone aglycone (luteolin) was found in the extract hydrolysates from the leaves. Kaempferol glycosides are predominant phenolic compounds in S. pallasii inflorescences, and quercetin glycosides in its leaves

Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro

Sorbaria pallasii is an endemic species of the Far East and Siberia and grows along the Goltsy altitudinal belt. Data on micropropagation and phytochemical characteristics of this plant are not available, probably because of the inaccessibility of the plant material. Morphogenesis initiation from flower buds of S. pallasii in vitro and micropropagation were performed here in the Murashige and Skoog medium supplemented with 5.0 µM 6-benzylaminopurine and 0.0–1.0 µM α-naphthylacetic acid; elongation was implemented in the same medium without the hormones. A well-growing sterile culture of S. pallasii was obtained; the number of microshoots per explant reached 5.7 ± 1.2. Phytochemical analyses of in vitro propagated S. pallasii detected 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in a water-ethanol extract from its microshoots and revealed phenolic compounds in it. The phenolic compounds that likely contribute to its biological activity are tannins (74.9 mg/g), phenolcarboxylic acids (30.8 mg/g), and catechins (13.3 mg/g). In the microshoot extract, high-performance liquid chromatography identified three catechins. Microshoots showed the highest concentration of (±)-catechin (3.03 mg/(g of absolutely dry mass; ADM)). Concentrations of epigallocatechin gallate (0.38 mg/(g of ADM)) and (−)-epicatechin (0.55 mg/(g of ADM)) were significantly lower. This study paves the way for further biotechnological and phytochemical research on S. pallasii