Phytochemical Screening, Antimicrobial Potential and GC-MS Analysis of Chloroform Leaf Extract of Hyptis suaveolens (L.) Poit

Bioactive compounds possessing therapeutic value are used for long time by vast group of researchers to treat various diseases for the goodness of the mankind. Most of the commercially available bioactive compounds are derived from plants because of their affirmative property on human health with minimum side effects. Hyptis suaveolens (L.) Poit. is one of the most important traditional and therapeutic plant, which is belongs to Lamiaceae family. The Biochemical test of the leaf extract of Hyptis suaveolens using different solvent shows the presence of biologically significant compounds. Thereby, the Antimicrobial properties of leaf extract of H. suaveolens carried out against six different bacterial pathogens and three fungi pathogens.  Most potential activity was observed in the crude extract of Hexane, Ethyl acetate and Chloroform against E.coli, Enterococcus faecalis and Streptococcus pyrogenes respectively when compared to Klebshilla pneumonia. Among the other crude extract, the chloroform crude extract resulted with more number of molecules in the phytochemical analysis and also it exhibits superior antibacterial activity. Hence, the Gas Chromatography-Mass Spectrometry (GC-MS) analysis of chloroform extract was achieved and 17 different bioactive compounds were discovered.  Neophytadiene, (S,Z)-Heptadeca-1,9-dien-4,6-diyn-3-ol, 2,3-Diazabicyclo[2.2.1]hept-2-ene, 7,7-dime and  Stigmasterol are the significant molecules present in the chloroform leaf extract it has the highest value for the mankind

Biocontrol Properties and Functional Characterization of Rice Rhizobacterium Pseudomonas sp. VSMKU4036

A total of 30 fluorescent pseudomonads (FPs) were showed significant antagonistic activity against different fungal phytopathogens with different level of the zone of inhibition (ZOI) for Rhizoctonia solani (5mm-34mm), Macrophomina phaseolina (9mm-37mm), Scleotium rolfsii (4mm-36mm), Helminthusporium solani (5mm-27mm), Fusarium oxysporum (2mm-25mm) and Fusarium oxysporum RACE (4mm-31mm) compared to control. The maximum growth of our selected isolate VSMKU4036 was observed in King’B Broth (KBB), pH 7.0 and at 37°C. The VSMKU4036 isolate has been recognized as Pseudomonas sp, based on the morphological, biological, and different functional characteristics. Antagonistic rhizobacterium Pseudomonas sp VSMKU4036 produced antimicrobial traits, such as plant growth promotion and various functional characters like siderophores, hydrogen cyanide (HCN), phosphate solubilization, indole acetic acid (IAA), biofilms formation, protease, gelatinase, amylase, and pectinase. Our superior biocontrol isolate VSMKU4036 was high resistance to tetracycline, streptomycin and nalidixic acid, however, it was sensitive to ampicillin and rifamycin. Pseudomonas sp VSMKU4036 showed maximum resistance to cadmium, nickel chloride, copper sulphate, magnesium sulphate, zinc chloride and ferric chloride where as highly sensitive to mercuric chloride, and selenium dioxide compared to control