How to manage gametes and embryos from virus carrier couples ?

info:eu-repo/semantics/nonPublishe

HCV Seropositivity impaired in vitro fertilization outcome

info:eu-repo/semantics/publishe

Cryopreservation of human failed maturation oocytes shows that vitrification gives superior outcomes to slow cooling.

This study investigated whether failed maturation oocytes could be used to evaluate different cryopreservation procedures. A total of 289 failed maturation oocytes (GV and MI stages), obtained from 169 patients undergoing IVF treatment (mean age 33.84±5.0) were divided into two different slow-cooling groups (1.5 mol/l 1,2-propanediol+0.2 mol/l sucrose in either NaCl (group A) or choline chloride (ChCl) (group B) based cryopreservation solutions) and one vitrification group (15% ethylene glycol+15% dimethyl sulphoxide). Survival rate, in vitro maturation (IVM) rate, fertilization and developmental rate of cryopreserved oocytes were assessed. Regardless of the stage at which cryopreservation was performed (GV+MI), the slow cooling with ChCl based medium always gave significantly lower survival rate than the slow cooling in NaCl based medium (p=0.01) and vitrification (p<0.001). An extended study also showed statistically reduced survival rate between slow-cooling NaCl based medium and vitrification (p<0.05). Global results of in vitro maturation and fertilization showed worse results between both slow-cooling NaCl and ChCl based media versus vitrification. In conclusion, for oocytes that had failed to mature, vitrification gave better survival, maturation, fertilization and also cleavage rates than the slow-cooling protocols. Four cells embryos were obtained only from vitrified in vitro matured MI oocytes.Journal ArticleResearch Support, Non-U.S. Gov'tSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Development and evaluation of single sperm washing (SSW) for decontamination in extreme oligospermic HIV positive patients

info:eu-repo/semantics/nonPublishe

Development and evaluation of single sperm washing for risk reduction in artificial reproductive technology (ART) for extreme oligospermic HIV positive patients

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Development and evaluation of a qualitative reverse-transcriptase nested polymerase chain reaction protocol for same-day viral validation of human immunodeficiency virus type 1 ribonucleic acid in processed semen

Objective: To develop a method for same-day validation of processed semen in the setting of assisted reproductive techniques (ART) with patients who are seropositive for human immunodeficiency virus, type 1 (HIV-1). Design: Laboratory experiments. Setting: University hospital. Patient(s): Volunteers who are HIV-1 seronegative and seropositive. Intervention(s): Evaluation of the sensitivity of a reverse-transcriptase (RT)-nested polymerase chain reaction (PCR) in HIV-1 RNA-positive blood plasma, in artificially infected blood plasma and semen, and in 85 semen samples of 29 HIV-1-seropositive volunteers. Semen was submitted to gradient separation, followed by swim-up. Main Outcome Measure(s): Qualitative detection of HIV-1 RNA in blood plasma and in different parts of semen preparation by using RT-nested PCR, PCR inhibition control by dilution of samples, and an internal control. Result(s): The detection limit of our PCR was 20 HIV-1 RNA copies per milliliter. Among seropositive patients, RNA was detected in 25% of fresh semen, 36.5% of seminal plasma, 27.5% of gradient supernatants, and 7.1% of final preparations before the migration-sedimentation stage. Positive final preparations were observed in patients who had blood viral loads of ≥20,000 HIV-1 RNA copies per milliliter. Inhibition was present in 17.6% of seminal plasma and in 20% gradient supernatants and in 2 final preparations among 69 tested. Among 25 preparations tested after the migration-sedimentation stage, 2 were positive (1 patient; 70,000 HIV-1 RNA copies per milliliter). Conclusion(s): The RT-nested PCR detects low viral load and allows the validation of semen preparations of HIV-1-seropositive patients for ART on the day of sampling. For this purpose, the validation is performed on spermatozoa that are obtained after gradient separation before swim-up. Inhibition of the PCR must be controlled by using an internal control that is well-designed to explore the detection limit of the method. © 2006 American Society for Reproductive Medicine.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Impaired ovarian stimulation during in vitro fertilization in women who are seropositive for hepatitis C virus and seronegative for human immunodeficiency virus

Objective: To analyze the impact of seropositivity with hepatitis C virus (HCV) on in vitro fertilization (IVF) outcomes. Design: Retrospective, case-controlled study. Setting: Fertility clinic of academic hospital. Patient(s): 42 IVF/intracytoplasmic sperm injection cycles in HCV-seropositive women and 84 matched control cycles. Intervention(s): IVF/intracytoplasmic sperm injection treatment for infertility. Main Outcome Measure(s): Ovarian response to stimulation, laboratory findings, and implantation and pregnancy rates. Result(s): Absence of ovarian response was statistically significantly higher for HCV-seropositive women compared with controls (10/42 vs 5/84 cycles, respectively). For cycles with oocyte retrieval, HCV-seropositive women required more gonadotropin units compared with controls. The maximum estradiol levels and number of collected oocytes were similar, but HCV-seropositive women had statistically significantly fewer embryos available compared with controls. Embryo morphologic features, number of transferred embryos, and rates of implantation and pregnancy were similar for HCV-seropositive women and controls. Conclusion(s): When compared with matched uninfected controls, HCV-seropositive women display a decreased ovarian response. © 2007 American Society for Reproductive Medicine.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Quality survey of embryo culture performances of two incubators

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Diminuer le risque de grossesse multiple en fecondation in vitro: Du transfert selectif de 2 embryons a cului d'un seul blastocyste ?

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Comparison of ethylene glycol, 1,2-propanediol and glycerol for cryopreservation of slow-cooled mouse zygotes, 4-cell enmbryos and blastocysts

SCOPUS: ar.jinfo:eu-repo/semantics/publishe