DEA 1.1. krvne grupe hrvatskih autohtonih pasmina pasa: posavskog goniča i tornjaka.

The study included 60 clinically healthy dogs of two Croatian indigenous breeds: the Posavaz Hound and the Tornjak Hound. Their ages ranged from 2 months to 8 years. Determination of blood group DEA 1.1. (Dog Erythrocyte Antigen) was performed using tests based on serological agglutination reaction. The blood of dogs that showed a positive reaction to the blood group DEA 1.1., intensity of agglutination (low, medium and strong) was determined. The Posavaz Hound breed showed 60% of dogs positive for DEA 1.1. group, and the Tornjak Hound breed showed 53.3% positive. In the Tornjak Hounds the strongest positive reaction was found for the largest number of individuals (56.3%), while in the Posavaz Hound breed for most individuals the lowest positive response was determined (55.6%). The strongest intensity of reaction was found in Tornjak Hound females and the lowest in Posavaz Hound males. Using Kruskal-Wallis and Chi-square tests no statistical difference was determined between the positive reaction intensity of Posavaz and Tornjak Hound DEA 1.1. blood groups.Istraživanjem je bilo obuhvaćeno 60 klinički zdravih pasa dviju hrvatskih autohtonih pasmina: posavski gonič (15 ženki i 15 mužjaka u dobi od 7 mjeseci do 8 godina) i tornjak (18 ženki i 12 mužjaka u dobi od 2 mjeseca do 7 godina). Uzorak krvi uzet je iz krvne žile v. cephalica u epruvete s EDTA antikoagulansom. Nakon vađenja krvi određena je moguća reakcija autoaglutinacije, koja je u svim uzorcima bila negativna. Određivanje krvne grupe DEA 1.1. (Dog Erythrocyte Antigen) obavljeno je pomoću serološkog testa RAPIDVET®-H (Canine DEA 1.1., dms/agrolabo products ag Neuhausen am Rheinfall, Switzerland), temeljenog na serološkoj reakciji aglutinacije koja nastupa kada eritrociti pasa koji sadrže DEA 1.1. antigen dođu u dodir s mišjim monoklonskim protutijelima. U krvi pasa koji su pokazali pozitivnu reakciju na krvnu grupu DEA 1.1., određena je jakost aglutinacije (slaba, srednja jak i jaka). Utvrđeno je 60% pozitivnih pasa na DEA 1.1. grupu u posavskog goniča, a 53,3% u tornjaka. Jaka reakcija zabilježen je u 56,3% pasa pasmine tornjak, dok je većina pozitivnih pasa pasmine posavski gonič imala slabu reakciju (55,6%). Najjača reakcija utvrđena je u ženki tornjaka, a najslabija u mužjaka posavskog goniča. Primjenom Kruskal-Wallis i Hi kvadrat testa nije utvrđena statistički značajna razlika