A Student¿s Project: Choices in Media for Communication and Presentation

Architectur

Architectural and urban simulation techniques in research and education: Developments of analogue and digital eye level visualisation

ArchitectureArchitectur

Visualising by Means of Endoscope, Computer and Hand-Drawn Techniques

Architectur

Design communication and image processing

Architectur

IMAG(IN)ING, a fresh look at design, presentation and communication

Architectur

Overdracht en simulatie: Onderzoek naar waarde en betekenis van mediatoepassing bij architektonische simulaties

Onderzoek naar waarde en betekenis van mediatoepassing bij architektonische simulaties.ArchitectureDelft University of Technolog

Vervolg onderzoek filters

Zoals bekend wordt in nota DREMBU-M-78023 (Eindrapport Drempelontwerp) voor het fundatie bed de oplossing, bestaande uit een laag grof zand van 0,3 - 1 a 2 mm en een laag gegradeerd materiaal van 0,3 - 32 mm voorgesteld.(zie voor de korrelverdelingen bijlage 1). V~~r de beoordeling van de filterwerking van deze oplossing was het noodzakelijk een groot aantal proeven uit te voeren, omdat in de literatuur niet of nauwelijks filterregels beschikbaar waren, die geldig waren voor de extreme belastingen (cyclische verhangen met amplituden tot circa 400 %), zoals die in het geval van de stormvloedkering waren berekend. parallel aan dit typisch -:modelonderzoek werd evenwel een uitgebreide literatuurstudie opgestart, teneinde meer inzicht te krijgen in hetgeen wel beschikbaar is en om het mechanisme beter te begrijpen. In deze nota worden deze activiteiten geëvalueerd en wordt tevens een aanpak voorgesteld, waarmee het ontwerp van het fundatie bed nader onderbouwd kan worden.Deltawerken, Oosterscheld

Direct observation of end resection by RecBCD during double-stranded DNA break repair in vivo

The formation of 3 single-stranded DNA overhangs is a first and essential step during homology-directed repair of double-stranded breaks (DSB) of DNA, a task that in Escherichia coli is performed by RecBCD. While this protein complex has been well characterized through in vitro single-molecule studies, it has remained elusive how end resection proceeds in the crowded and complex environment in live cells. Here, we develop a two-color fluorescent reporter to directly observe the resection of individual inducible DSB sites within live E. coli cells. Realtime imaging shows that RecBCD during end resection degrades DNA with remarkably high speed (i"1.6 kb/s) and high processivity (>7sim;100 kb). The results show a pronounced asymmetry in the processing of the two DNA ends of a DSB, where much longer stretches of DNA are degraded in the direction of terminus. The microscopy observations are confirmed using quantitative polymerase chain reaction measurements of the DNA degradation. Deletion of the recD gene drastically decreased the length of resection, allowing for recombination with short ectopic plasmid homologies and significantly increasing the efficiency of horizontal gene transfer between strains.We thus visualized and quantified DNA end resection by the RecBCD complex in live cells, recorded DNA-degradation linked to end resection and uncovered a general relationship between the length of end resection and the choice of the homologous recombination template.BN/Cees Dekker LabApplied Science

Controlling Healing and Toughness in Polyurethanes by Branch-Mediated Tube Dilation

In this work, we propose the use of regular branching of polyurethanes as a way to regulate chain dynamics and govern crystallization in highly dense hydrogen-bonded systems. As a result, robust and healable polyurethanes can be obtained. To this end, we synthesized a range of aliphatic propane diol derivatives with alkyl branches ranging from butyl (C4) to octadecanyl (C18). The series of brush polyurethanes was synthesized by polyaddition of the diols and hexamethylene diisocyanate. Polyurethanes with very short (C 4) and very long (C = 18) brush lengths did not lead to any significant healing due to crystallization. An intermediate amorphous regime appears for polymers with middle branch lengths (C = 4 to 8) showing a fine control of material toughness. For these systems, the side chain length regulates tube dilation, and significant macroscopic healing of cut samples was observed and studied in detail using melt rheology and tensile testing. Despite the high healing degrees observed immediately after repair, it was found that samples with medium to long length brushes lost their interfacial strength at the healed site after being heated to the healing temperature for some time after the optimal time to reach full healing. Dedicated testing suggests that annealed samples, while keeping initial tackiness, are not able to completely heal the cut interface. We attribute such behavior to annealing-induced interfacial crystallization promoted by the aliphatic branches. Interestingly, no such loss of healing due to annealing was observed for samples synthesized with C4 and C7 diols, which is identified as the optimal healing regime. These results point at the positive effect of branching on healing, provided that a critical chain length is not surpassed, as well as the need to study healing behavior long after the optimal healing times.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Novel Aerospace Material

Printing of Patterned, Engineered E. coli Biofilms with a Low-Cost 3D Printer

Biofilms can grow on virtually any surface available, with impacts ranging from endangering the lives of patients to degrading unwanted water contaminants. Biofilm research is challenging due to the high degree of biofilm heterogeneity. A method for the production of standardized, reproducible, and patterned biofilm-inspired materials could be a boon for biofilm research and allow for completely new engineering applications. Here, we present such a method, combining 3D printing with genetic engineering. We prototyped a low-cost 3D printer that prints bioink, a suspension of bacteria in a solution of alginate that solidifies on a calcium-containing substrate. We 3D-printed Escherichia coli in different shapes and in discrete layers, after which the cells survived in the printing matrix for at least 1 week. When printed bacteria were induced to form curli fibers, the major proteinaceous extracellular component of E. coli biofilms, they remained adherent to the printing substrate and stably spatially patterned even after treatment with a matrix-dissolving agent, indicating that a biofilm-mimicking structure had formed. This work is the first demonstration of patterned, biofilm-inspired living materials that are produced by genetic control over curli formation in combination with spatial control by 3D printing. These materials could be used as living, functional materials in applications such as water filtration, metal ion sequestration, or civil engineering, and potentially as standardizable models for certain curli-containing biofilms.Accepted Author ManuscriptBN/Marie-Eve Aubin-Tam LabBN/Timon Idema LabBN/Anne Meyer La