Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment

Planarians are flatworms capable of regenerating any missing body region. This capacity is mediated by neoblasts, a proliferative cell population that contains pluripotent stem cells. Although population-based studies have revealed many neoblast characteristics, whether functionally distinct classes exist within this population is unclear. Here, we used high-dimensional single-cell transcriptional profiling from over a thousand individual neoblasts to directly compare gene expression fingerprints during homeostasis and regeneration. We identified two prominent neoblast classes that we named ζ (zeta) and σ (sigma). Zeta-neoblasts encompass specified cells that give rise to an abundant postmitotic lineage including epidermal cells, and are not required for regeneration. By contrast, sigma-neoblasts proliferate in response to injury, possess broad lineage capacity, and can give rise to zeta-neoblasts. These findings present a new view of planarian neoblasts, in which the population is comprised of two major and functionally distinct cellular compartments.Human Frontier Science Program (Strasbourg, France)National Institutes of Health (U.S.) (Grant R01GM080639

Artificial neural networks for 3D cell shape recognition from confocal images

We present a dual-stage neural network architecture for analyzing fine shape details from microscopy recordings in 3D. The system, tested on red blood cells, uses training data from both healthy donors and patients with a congenital blood disease. Characteristic shape features are revealed from the spherical harmonics spectrum of each cell and are automatically processed to create a reproducible and unbiased shape recognition and classification for diagnostic and theragnostic use.Comment: 17 pages, 8 figure

Bayesian Analysis of Two Stellar Populations in Galactic Globular Clusters II: NGC 5024, NGC 5272, and NGC 6352

We use Cycle 21 Hubble Space Telescope (HST) observations and HST archival ACS Treasury observations of Galactic Globular Clusters to find and characterize two stellar populations in NGC 5024 (M53), NGC 5272 (M3), and NGC 6352. For these three clusters, both single and double-population analyses are used to determine a best fit isochrone(s). We employ a sophisticated Bayesian analysis technique to simultaneously fit the cluster parameters (age, distance, absorption, and metallicity) that characterize each cluster. For the two-population analysis, unique population level helium values are also fit to each distinct population of the cluster and the relative proportions of the populations are determined. We find differences in helium ranging from $\sim$0.05 to 0.11 for these three clusters. Model grids with solar $\alpha$-element abundances ([$\alpha$/Fe] =0.0) and enhanced $\alpha$-elements ([$\alpha$/Fe]=0.4) are adopted.Comment: ApJ, 21 pages, 14 figures, 7 table

Optimizing information flow in small genetic networks. II: Feed forward interactions

Central to the functioning of a living cell is its ability to control the readout or expression of information encoded in the genome. In many cases, a single transcription factor protein activates or represses the expression of many genes. As the concentration of the transcription factor varies, the target genes thus undergo correlated changes, and this redundancy limits the ability of the cell to transmit information about input signals. We explore how interactions among the target genes can reduce this redundancy and optimize information transmission. Our discussion builds on recent work [Tkacik et al, Phys Rev E 80, 031920 (2009)], and there are connections to much earlier work on the role of lateral inhibition in enhancing the efficiency of information transmission in neural circuits; for simplicity we consider here the case where the interactions have a feed forward structure, with no loops. Even with this limitation, the networks that optimize information transmission have a structure reminiscent of the networks found in real biological systems

Solid-phase C60 in the peculiar binary XX Oph?

We present infrared spectra of the binary XX Oph obtained with the Infrared Spectrograph on the Spitzer Space Telescope. The data show some evidence for the presence of solid C60– the first detection of C60 in the solid phase – together with the well-known ‘unidentified infrared’ emission features. We suggest that, in the case of XX Oph, the C60 is located close to the hot component, and that in general it is preferentially excited by stars having effective temperatures in the range 15 000–30 000 K. C60 may be common in circumstellar environments, but unnoticed in the absence of a suitable exciting source

Comparison of optical probes and X-ray tomography for bubble characterization in fluidized bed methanation reactors

The performance of many fluidized bed reactors strongly depends on the bubble behavior since they influence the mass transfer to the dense phase where the catalyst is present. An example is the methanation in a fluidized bed that allows for conversion of unsaturated hydrocarbons in the gasification gas without catalyst deactivation [1]. The BFB reactor is a very challenging step in the process chain to produce SNG out of biomass as feedstock since next to the bubble behavior a lot of other parameters like temperature, pressure, particle size, attrition of the catalyst, internals, bed height and reactor diameter etc. affect the overall performance. The focus of this research work lies on the determination of the bubble properties which are an important factor to model a bubbling fluidized methanation reactor in order to predict and optimize its performance and to support its scale-up [2]. Tomographic methods such as X-ray measurements are often used to characterize bubbles in a fluidized bed. Compared to intrusive measurement, e.g. optical probing, this method possesses the advantage of measuring bubbles throughout the entire cross section. However, X-ray measurements cannot be applied to all installation, especially not in large scale plants. For these purpose, we have developed optical probes that can be employed to investigate the fluidization state in a hot pilot scale reactor. A main drawback of the optical measurements lies in their locally limited detection of the hydrodynamic pattern since they are only able to measure at one point in the reactor. Therefore, conclusions on the bubble behavior of the whole cross section based on optical measurements are not easy to derive. To compare the influence of the measurement method on the measured bubble properties, in the scope of this study, an artificial optical signal is created out of the existing X-ray measurement data set for a cold flow model of the pilot scale methanation reactor. The obtained bubble properties of both methods (i.e. evaluation of the derived artificial optical probe signal and image reconstruction based on the original X-ray tomographic data) are compared with regard to the hold-up, bubble rise velocity and the bubble size (for the X-ray method) or chord length (for the optical evaluation method), respectively. The process to obtain an artificial optical signal is depicted in Figure 1. The comparison shows that for the evaluation of optical probe data, statistical effects have to be considered carefully. The detected mean chord length of the optical method does not represent the mean bubble size determined by the X-ray method. Moreover, also a difference in the bubble rise velocity was detected for some fluidization states. This knowledge may be the basis for the derivation of a statistically sound method to calculate different hydrodynamic properties in fluidized bed reactors based on optical probe measurements. Please click Additional Files below to see the full abstract

Thermal Suppression of Strong Pinning

We study vortex pinning in layered type-II superconductors in the presence of uncorrelated disorder for decoupled layers. Introducing the new concept of variable-range thermal smoothing, we describe the interplay between strong pinning and thermal fluctuations. We discuss the appearance and analyze the evolution in temperature of two distinct non-linear features in the current-voltage characteristics. We show how the combination of layering and electromagnetic interactions leads to a sharp jump in the critical current for the onset of glassy response as a function of temperature.Comment: LaTeX 2.09, 4 pages, 2 figures, submitted to Phys. Rev. Let

Cavity effects on the Fermi velocity renormalization in a graphene sheet

Recently, in the literature, it was shown that the logarithmic renormalization of the Fermi velocity in a plane graphene sheet (which, in turn, is related to the Coulombian static potential associated to electrons in the sheet) is inhibited by the presence of a single parallel conducting plate. In the present paper, we investigate the situation of a suspended graphene sheet in a cavity formed by two conducting plates parallel to the sheet. The effect of a cavity on the interaction between electrons in the graphene is not merely the addition of the effects of each plate individually. From this, one can expect that the inhibition of the renormalization of the Fermi velocity generated by a cavity is not a mere addition of the inhibition induced by each single plate. In other words, the simple addition of the result for the inhibition of the renormalization of the Fermi velocity found in the literature for a single plate could not be used to predict the exact behavior of the inhibition for the graphene between two plates. Here, we show that, in fact, this is what happens and calculate how the presence of a cavity formed by two conducting plates parallel to the suspended graphene sheet amplifies, in a non-additive manner, the inhibition of the logarithmic renormalization of the Fermi velocity. In the limits of a single plate and no plates, our formulas recover those found in the literature.This work was partially supported by the following Brazilian Agencies: Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ). E. C. Marino was partially supported by CNPq and FAPERJ. D. T. Alves was partially supported by CAPES via Programa Estagio Senior no Exterior - Processo 88881.119705/2016-01, by CNPq via Processos 461826/2014-3 (Edital Universal) and 311920/2014-4 (Bolsa de Produtividade em Pesquisa), and also thanks Jaime Santos, Mikhail I. Vasilevskiy, Nuno M. R. Peres and Yuliy Bludov for useful discussions, as well as the hospitality of the Centro de Fisica, Universidade do Minho, Braga - Portugal. V. S. Alves acknowledges CNPq for support through Bolsa de Produtividade em Pesquisa n. 312654/2017-0. The authors also thank Ygor P. Silva for useful comments

Observation of macroscopic Landau-Zener transitions in a superconducting device

A two-level system traversing a level anticrossing has a small probability to make a so-called Landau-Zener (LZ) transition between its energy bands, in deviation from simple adiabatic evolution. This effect takes on renewed relevance due to the observation of quantum coherence in superconducting qubits (macroscopic "Schrodinger cat" devices). We report an observation of LZ transitions in an Al three-junction qubit coupled to a Nb resonant tank circuit.Comment: REVTeX4, 4pp., 4 EPS figures. v2: clarifications added; final, to appear in EP