Development and validation of a fast gas chromatography combustion isotope ratio mass spectrometry method for the detection of epiandrosterone sulfate in urine

In doping control, to confirm the exogenous origin of exogenously administered anabolic androgenic steroids (AAS), a gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) analysis is performed. Recently published work suggests that epiandrosterone sulfate (EpiAS) is a promising IRMS target compound for the detection of AAS, capable of prolonging the detection window. However, EpiAS is only excreted in urine in its sulfoconjugated form, while all other IRMS target compounds are excreted glucuronidated, meaning that EpiAS cannot be incorporated in the existing IRMS methods. A separate extensive sample preparation needs to be performed on this compound with a different hydrolysis and extraction procedure and a different liquid chromatography (LC) clean-up. The current work presents a new, fast, and easy to implement EpiAS IRMS method. The approach was based on the direct GC analysis of non-hydrolyzed EpiAS, making the solid phase extraction, hydrolysis, and acetylation step redundant. Sample preparation consisted of a simple liquid-liquid extraction, followed by LC fraction collection. A population study was performed to check compliance with the criteria drafted by the World Anti-Doping Agency (WADA). To verify the applicability of the developed approach, the method was applied to the samples of four administration studies (i.e. dehydroepiandrosterone (DHEA), testosterone gel (T gel), androstenedione (ADION), and intramuscular testosterone undecanoate. In contrast to previously published data, the strength of EpiAS as the target compound and the prolongation of the detection window in comparison with the conventional IRMS target compounds was less pronounced

Porous stones increase the noise shielding of a gabion

Gabions - metal-wired cages filled up with stones - are increasingly becoming popular as decorative elements and land property boundaries. It has been shown before that such structures can be used as road traffic noise barriers as well. However, the types of stones used in gabions have not been experimentally studied so far. Exploratory measurements at full scale in a semi-anechoic room were performed to study the effect of both porous and rigid stones on their noise reducing potential. At the 1/3 octave bands below 1 kHz, low-height gabions (with depths of 20 cm and 30 cm) hardly provide any sound pressure level reduction. At higher sound frequencies, in contrast, the shielding rapidly increases. Porous lava stones were found to significantly increase the shielding compared to rigid stones. Reflections on such nondeep low-height barriers towards the source side were found to be of minor importance when considering a standardized A-weighted road traffic noise spectrum. (C) 2018 Elsevier Ltd. All rights reserved