Synthesis of antiulcer drug esomeprazole.

Esomeprazole (Nexium®), the (S)-isomer of Omeprazole, is the first proton-pump inhibitor developed as a single isomer for the treatment of acid-related diseases. It is used for the treatment of peptic ulcers, gastroesophagal reflux disease, and erosive esophagitis. Herein, we report our synthetic study of esomeprazole sodium salt from the starting    2-mercapto-5-methoxybenzimidazole and 2-(chloromethyl)-4-methoxy-3,5-dimethylpyridine hydrochloride reagents. The Esomeprazole sodium salt was obtained from enantioselectivesulfoxidation reaction in moderate yield with high enantioselectivity

Isolation, screening antimicrobial activity and identification of fungi from marine sediments of the area Thanh Lan, Co To, Vietnam

Marine environment is rich in natural product resources, including marine microorganisms, especially fungi which are not only seen as a potential source of highly applicable bioactive substances but also can provide for science new chemical structures. The objective of this study is to isolate and screen fungal strains with antibacterial activity from the marine environment. Twenty five strains of fungi were isolated from marine sediments of Thanh Lan, Co To island and assessed on antibiotic activity against 7 tested microbial strains, including three Gram-negative bacteria (Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Salmonella enterica ATCC13076), three Gram-positive bacteria (Enterococcus faecalis ATCC29212, Stapphylococus aureus ATCC25923, Bacillus cereus ATCC 13245), and the yeast Candida albicans ATCC10231. The minimum inhibitory concentration (MIC) against the tested microorganisms was determined for the crude extracts obtained from the culture broths after ethyl acetate extraction and vacuum rotary evaporation. Three strains with the highest antimicrobial activity M26, M30 and M45 were capable of inhibiting 4 - 5 of the 7 tested microorganisms with MIC values from 64 to 256 μg/ml, depending on each tested strain. Morphological and phylogenetic investigations based on 18S rRNA gene sequences of the three selected strains showed that strains M26 and M30 belonged to the genus Penicillium, whereas strain M45 belonged to the genus Neurospora. The sequences of 18S rRNA gene of three strains M26, M30 and M45 were registered on GenBank database with accession numbers: MH673730, MH673731, MH673732, respectively. Research results showed that marine environment has a great potential in isolation of fungal strains for the search for antibacterial substances as well as other biologically active compounds

Impacts of fallow conditions, compost and silicate fertilizer on soil nematode community in salt–affected paddy rice fields in acid sulfate and alluvial soils in the Mekong Delta, Vietnam

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)Avoidance of intensive rice cultivation (IRC) and soil amendments are potential practices to enhance soil properties. There is only limited information on the effects of reduced IRC and its mixture with compost or silicate fertilizer (Si) on the soil nematode community in salt–affected soils. This study aimed to assess the shifts of soil nematode community by reducing a rice crop from triple rice system (RRR) to a double rice system and mixed with compost or Si in paddy fields in acid sulfate soil (ASS) and alluvial soil (AL) in the Mekong Delta, Vietnam. Field experiments were designed with four treatments in four replicates, including RRR and a proposed system of double–rice followed by a fallow (FRR) and with 3 Mg ha–1 crop−1 compost or 100 kg ha–1 crop−1 Si. Soils were collected at harvest after the 2 year experiment, reflecting the fifth and third consecutive rice crop in RRR and FRR system, respectively. Results showed that reduced IRC gave a significant reduction in abundance of plant–parasitic nematodes (PPN), dominated by Hirschmanniella and increased abundance bacterivorous nematodes when mixed to compost and silicate fertilizer in ASS. In addition, reduced IRC increased nematode biodiversity Hill’s indices and reduced herbivorous footprint in ASS. Proposed system having compost or Si had strongly increased in bacterivorous and omnivorous footprints. Particularly, reduced IRC mixture with Si increased abundance of Rhabdolaimus, Mesodorylaimus and Aquatides, metabolic footprints (structure footprint, bacterivorous, omnivorous and predator) and diversity Hill’s N1 index in ASS. Our results highlighted that reduced IRC was a beneficial practice for decreasing abundance of PPN in salt-affected soils and increasing abundance of FLN in ASS. IRC mixture with compost or Si had potential in structuring the nematode communities with increasing biodiversity, trophic structure, and metabolic footprintsPeer reviewedFinal Accepted Versio

Temporal fluctuation of multidrug resistant salmonella typhi haplotypes in the mekong river delta region of Vietnam.

BACKGROUND: typhoid fever remains a public health problem in Vietnam, with a significant burden in the Mekong River delta region. Typhoid fever is caused by the bacterial pathogen Salmonella enterica serovar Typhi (S. Typhi), which is frequently multidrug resistant with reduced susceptibility to fluoroquinolone-based drugs, the first choice for the treatment of typhoid fever. We used a GoldenGate (Illumina) assay to type 1,500 single nucleotide polymorphisms (SNPs) and analyse the genetic variation of S. Typhi isolated from 267 typhoid fever patients in the Mekong delta region participating in a randomized trial conducted between 2004 and 2005. PRINCIPAL FINDINGS: the population of S. Typhi circulating during the study was highly clonal, with 91% of isolates belonging to a single clonal complex of the S. Typhi H58 haplogroup. The patterns of disease were consistent with the presence of an endemic haplotype H58-C and a localised outbreak of S. Typhi haplotype H58-E2 in 2004. H58-E2-associated typhoid fever cases exhibited evidence of significant geo-spatial clustering along the Sông H u branch of the Mekong River. Multidrug resistance was common in the established clone H58-C but not in the outbreak clone H58-E2, however all H58 S. Typhi were nalidixic acid resistant and carried a Ser83Phe amino acid substitution in the gyrA gene. SIGNIFICANCE: the H58 haplogroup dominates S. Typhi populations in other endemic areas, but the population described here was more homogeneous than previously examined populations, and the dominant clonal complex (H58-C, -E1, -E2) observed in this study has not been detected outside Vietnam. IncHI1 plasmid-bearing S. Typhi H58-C was endemic during the study period whilst H58-E2, which rarely carried the plasmid, was only transient, suggesting a selective advantage for the plasmid. These data add insight into the outbreak dynamics and local molecular epidemiology of S. Typhi in southern Vietnam

Factors affecting the decision to choose a university of high school students: A study in An Giang Province, Vietnam

It is important to provide high school students with the necessary information for them to consult and make a decision to choose a university. The study aims to identify and evaluate the influence of factors in the decision to choose a university for high school students. The questionnaire survey method was used to collect data from 393 students from eight high schools in An Giang Province, Vietnam. Exploratory factor analysis and linear regression were used to analyze the data. The research results show that students are quite satisfied and quite certain with their decision to choose a university, while there are six important factors affecting the decision to choose a university. Influential factors with decreasing order of magnitude are: i) Factors consulted by teachers, family, friends, and relatives; ii) Factors of future job opportunities; iii) Factors of media activities; iv) Factors of learning conditions; v) Factors of university reputation; vi) Factors belong to the students themselves. The findings of the study show that there is no statistically significant difference between the group of males and females, between grades 10, 11, and 12. Besides, there is a statistically significant difference between students in high schools. The findings of this study have theoretical and practical implications for university admissions in Vietnam. Proposals made to university administrators were discussed. From the research results, we want to help students find the right university, and support universities to improve the efficiency of admissions

Triterpenes and triterpene-glycoside from the leaves of Lawsonia inermis.

From the leaves of  Lawsonia inermis (syn. L. alba), two triterpenes augustic acid (1) and 1b,2a,3a,19a-tetrahydroxy-12-ursen-28-oic acid (2), and a triterpene-glycoside suavissimoside R1 (3) were isolated by using various chromatoghraphies. Their structures were characterized on the basis of the spectroscopic data (1D-NMR, HSQC, HMBC, ESI-MS) in comparison with the literature. This is the first report of 1 - 3 from Lawsonia species. Keywords: Lawsonia inermis, Lythraceae, Triterpene

Synthesis and biological evaluation of shikimic acid derivatives

From shikimic acid, new series of oseltamivir analogues containing either ether sulfur or ether oxygen at C-3 position were prepared and evaluated for their biological activities. This is the first report on synthesis of oseltamivir analogues with ether sulfur at C-3. Except for compounds 4d, 4f, 7c and 7d, the remaining compounds were found to be active against MCF-7, LU-1 and KB cell lines. As oseltamivir had no cytotoxicity against different cell lines such as MCF-7, LU-1, KB, MDCK, MRC-5, VERO, MK and 293, the modification of alkyl groups at C-3 of the oseltamivir ring framework could significantly increase the cytotoxicity for this class of compounds. Loss of neuraminidase inhibition activity of these synthetic oseltamivir analogues suggested that the alkyl ether groups at C-3 should be critical for their anti-neuraminidase activity

Potential Use of Rice Husk Biochar and Compost to Improve P Availability and Reduce GHG Emissions in Acid Sulfate Soil

© 2020 by the authors. Acid sulfate soil (ASS) has major problems related to phosphorus deficiency and high potential for N2O emissions, as well as strong acidity. The objective of this study was to evaluate the effects of rice husk biochar and compost on P availability and greenhouse gas (GHG) emissions in ASS in in vitro incubation studies. An ASS was amended with two types of rice husk biochar (at rates of 0 g kg-1, 20 g kg-1, and 50 g kg-1, equivalent to 0 Mg ha-1, 20 Mg ha-1, and 50 Mg ha-1, assuming that bulk density was 1 g cm-3 and evenly applied for 10 cm in depth) and compost (at rates of 0 g kg-1, 10 g kg-1, and 20 g kg-1, equivalent to 0 Mg ha-1, 10 Mg ha-1, and 20 Mg ha-1) and incubated. Application of compost increased labile P by 100% and 200% at rates of 10 g kg-1 and 20 g kg-1, respectively. Both biochars showed an increase in NaHCO3-soluble inorganic P by 16% to 30%, decreases in NaOH-soluble inorganic P and NaHCO3-soluble organic P. N2O emissions were significantly decreased by 80% by a biochar with a higher surface area and higher NH4+ adsorption capacity at a rate of 50 g kg-1 as compared with those in un-amended soil. In contrast, compost amendment at a rate of 10 g kg-1 significantly increased N2O emission by 150%. These results suggest that in ASS, whilst compost is more effective in improving P availability, biochar is more effective in mitigating GHG emissions, emphasizing that fundamental characteristics of organic amendments influenced the outcomes in terms of desirable effects

Prescription of antipsychotics and prevalence of metabolic syndrome in Vietnamese inpatients with schizophrenia

Schizophrenia is a severe mental illness, characterized by psychotic symptoms including delusions, hallucinations, dystonia, adolescent behavior and language, and is accompanied by severe functional impairments. Antipsychotics, which are used mainly in the treatment of schizophrenia, have contributed to the development of metabolic syndrome. Studies on metabolic syndrome in schizophrenic patients have been still limited in Vietnam. The objective of this study was to investigate the prevalence of metabolic syndrome in Vietnamese inpatients with schizophrenia. The study population of this descriptive cross-sectional study was all patients with schizophrenia from September 2020 to June 2021 at Inpatient Department of Binh Dinh Provincial Psychiatric Hospital in Viet Nam. Three hundred and twelve patients who met the sampling requirements were enrolled in this study. The prevalence of schizophrenia in female patients was lower than in male patients (36.9% vs. 63.1%) in our study. The prevalence of metabolic syndrome was 30.4% (25.9% in men and 38.3% in women), more in patients ≥40 years old than in patients under 40 years old (the highest prevalence in patients ≥60 years). There was a significant relationship between age, body mass index (BMI), and duration of antipsychotic treatment with the frequency of metabolic syndrome. In conclusions, the prevalence of schizophrenia was higher in male inpatients than in female inpatients. However, the prevalence of metabolic syndrome was higher in female inpatients than male inpatients with schizophrenia, and increased with age, BMI, and duration of antipsychotic treatment

Constituents from stem barks of Anacolosa poilanei Gagnep. (Olacaceae)

Four compounds were isolated from the stem barks of Anacolosa poilanei Gagnep. Theirs structures were established by spectroscopic analysis including MS and NMR. Accordingly, the isolates were identified as trichadenic acid B (1), trichadonic acid (2), amentoflavone (3) and β-sitosterol (4)