Additional file 1: of Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

RT-LAMP primers design for PEDV nucleotide detection. Nucleotide sequence alignments of M gene of seven PEDV strains. Representative M gene sequences in each strain are aligned with clustalW. Sequence data of designing primers for RT-LAMP in this study (KT323979.1), the sequence used for RT-PCR (JX435310.1 and JN089738.1), the sequence of G1b S INDEL strain (KY619833.1), the sequence of G2b/Non S INDEL/North America strain (KY619838.1), the sequence of G2a/Non S INDEL/Asian strain (KJ960178.1), the sequence of NK96P4C6 G1a classical strain (KY619828). Primer recognition sites are indicated with primer names. (DOCX 28 kb

Additional file 2: of Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

Detection limits of one-step RT-PCR and RtF-RT-LAMP for PEDV S INDEL field strain. From 5.0 × 105 to 5.0 × 100: tenfold serial dilution of 5.0 × 106 copies PEDV S INDEL field strain. (DOCX 14 kb

Additional file 3: of Development of pooled testing system for porcine epidemic diarrhoea using real-time fluorescent reverse-transcription loop-mediated isothermal amplification assay

Detection limits of one-step RT-PCR and RtF-RT-LAMP for the PEDV Non-S INDEL field strain. From 1.5 × 106 to 1.5 × 100: tenfold serial dilution of 1.5 × 107 copies PEDV Non-S INDEL field strain. (DOCX 14 kb