Omentopexy for correction of right abomasal displacement: results in 135 cows

One hundred and thirty-five cows were surgically treated for correction of right displaced abomasum (RDA) using the right flank omentopexy technique. In 33 cows an abomasal dilatation was diagnosed. Abomasal volvulus was found in 99 animals and omaso-abomasal volvulus in three. In-hospital mortality was 15% (n = 20). None of the cows with abomaso-omasal torsion survived. Ninety-seven percent (n = 32) of the cows with abomasal dilatation and 84% (n = 83) of the cows with abomasal volvulus were discharged from the clinic. Six months after surgery, respectively 94% of the cows with abomasal dilatation had survived. This percentage fell to 88.5% after another half year. For cows with abomasal volvulus, these survival rates were 74% and 62%, respectively. Regardless of the type of abomasal dislocation, 77% of the total group of animals survived after six months and 66% after one year. Six months after surgery, good milk production was reported in 67% (n = 58) of the surviving cows; this figure rose to 91% of the surviving cows (n = 63) after 12 months

The use of equine chondrogenic‐induced mesenchymal stem cells as a treatment for osteoarthritis : a randomised, double‐blinded, placebo‐controlled proof‐of‐concept study

Background: There is a need to improve therapies for osteoarthritis in horses. Objectives To assess the efficacy of equine allogeneic chondrogenic-induced mesenchymal stem cells combined with equine allogeneic plasma as a novel therapy for osteoarthritis in horses. Study design: Randomised, double-blinded, placebo-controlled experiment. Methods: In 12 healthy horses, osteoarthritis was induced in the metacarpophalangeal joint using an osteochondral fragment-groove model. Five weeks after surgery, horses were randomly assigned to either an intra-articular injection with chondrogenic-induced mesenchymal stem cells + equine allogeneic plasma (= intervention) or with 0.9% saline solution (= control). From surgery until the study end, horses underwent a weekly joint and lameness assessment. Synovial fluid was collected for cytology and biomarker analysis before surgery and at Weeks 5, 5 + 1d, 7, 9 and 11. At Week 11, horses were subjected to euthanasia, and the metacarpophalangeal joints were evaluated macroscopically and histologically. Results: No serious adverse events or suspected adverse drug reactions occurred during the study. A significant improvement in visual and objective lameness was seen with the intervention compared with the control. Synovial fluid displayed a significantly higher viscosity and a significantly lower glycosaminoglycan concentration in the intervention group. Other biomarkers or cytology parameters were not significantly different between the treatment groups. Significantly less wear lines and synovial hyperaemia were present in the intervention group. The amount of cartilage oligomeric matrix protein, collagen type II and glycosaminoglycans were significantly higher in the articular cartilage of the intervention group. Main limitations: This study assessed the short-term effect of the intervention on a limited number of horses, using an osteoarthritis model. This study also included multiple statistical tests, increasing the risk of type 1 error. Conclusions: Equine allogeneic chondrogenic-induced mesenchymal stem cells combined with equine allogeneic plasma may be a promising treatment for osteoarthritis in horses

Haemangiosarcoma in a captive Asiatic lion (Panthera leo persica)

A 2.7-year-old male captive Asiatic lion (Panthera leo persica) died unexpectedly without preceding symptoms. Gross necropsy revealed liver and lung tumours, which proved to be haemangiosarcomas by histopathology. Some of the liver tumours were ruptured, leading to massive intra-abdominal haemorrhage and death. Haemangiosarcomas are rare in domestic and exotic felids, occurring in skin, thoracic-abdominal cavity and bones. Although these tumours mainly appear to be occurring in older cats, they are sometimes observed in younger animals, as in the present case. This is the first description of haemangiosarcoma in a young Asiatic lion.Keywords: Asiatic lion, Haemangiosarcoma, Histopathology, Neoplasia, Panthera leo persic

The use of slaughterhouse-obtained small intestinal tissue as control material in histological studies should be applied with prudence

This study aimed to evaluate the reliability of slaughterhouse-obtained small intestinal tissue as control material in equine colic research where molecular stress responses in small intestinal tissue are investigated. For this purpose, small intestinal samples from colic horses were collected during surgery or immediately after euthanasia at the oral border of strangulation resection sites and routinely processed for histopathology (i.c. rinsed with 4°C Krebs’ solution, fixated overnight with 4% neutral buffered formaldehyde (FH) at room temperature). Control samples consisted of pieces of mid-jejunum, collected at the slaughterhouse and routinely processed for histopathology under 4 different conditions. The 4 conditions differed with regard to incubation and fixation temperature and whether or not oxygenated Krebs’ solution was used. Histological scoring revealed that slaughterhouse samples had a higher mean lesion score (P<0.001) than colic samples. In addition, more slaughterhouse samples had a higher mean inflammation score than colic samples (P=0.001). The inflammatory cells in the small intestine consisted mostly of eosinophils and as such were very suggestive for parasitic infestation. Hypoxia-inducible factor-1α (HIF1α) nuclear immunoreactivity was more pronounced in slaughterhouse tissue, probably as a result of the delay between slaughter and sampling (P=0.034). The histopathological score (P=0.291), the inflammation score (P=0.248) and the HIF1α nuclear immunoreactivity (P=0.538) did not differ between the different collection protocols. It is concluded that slaughterhouseobtained small intestinal tissue shows distinct alterations and that its use as control tissue when evaluating molecular stress responses should be applied with prudence

Morphological changes in the small intestinal smooth muscle layers of horses suffering from small intestinal strangulation: is there a basis for predisposition for reduced contractility?

Reasons for performing study: Intestinal strangulation often leads to enterectomy after which ileus can develop. This has prompted research to look into possible pathophysiological processes triggering equine ileus. However, morphological changes of the small intestinal smooth muscle in relation to equine colic have not yet been studied. Objectives: The presence of some smooth muscle proteins was morphologically assessed and quantified in control and colic horses. In addition, the up- or down-regulation of heat shock proteins (HSP20 and HSP27) influencing the contractility of smooth muscles was studied. Methods: Cranial resection margins of 18 strangulated small intestinal samples were collected. Small intestinal control samples were collected from 11 horses subjected to euthanasia for other than gastrointestinal-related reasons. Formaldehyde-fixed tissue was paraffin-embedded and processed for conventional staining and immunohistochemistry. Snap-frozen full-thickness biopsies were collected for western blot analyses. Results: Evaluating the muscle layer microscopically, colic samples showed significantly more signs of degradation than controls (P = 0.026) of which vacuolar degeneration was most prominent (P = 0.009). In colic samples, myosin protein levels were decreased (P = 0.022) whereas desmin (P = 0.049) and HSP20 protein levels (P = 0.005) were elevated. Conclusions: In colic samples, microscopic lesions at the level of the muscle layer indicate a stress response. In addition, modified amounts of structural proteins such as myosin and desmin together with increased HSP20 levels could perhaps provide a basis for explaining the malfunctioning of the intestinal muscle layer. Potential relevance: Post operative ileus, following small intestinal strangulation and resection, could be related in part to a dysfunctional muscle layer. In addition to microscopic signs of degeneration, myosin and HSP20 were affected. Pharmacological interventions might alter HSP20 expressions and thus serve a protective effect

Morphological data indicate a stress response at the oral border of strangulated small intestine in horses

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Sclerosing encapsulating peritonitis : a very rare disease entity in cats = Sclerosing encapsulating peritonitis : een zeer zeldzame ziekte bij katten

Sclerosing encapsulating peritonitis (SEP), also known as encapsulating peritoneal sclerosis, is a very rare disease described in humans as well as in animals. This condition is characterized by a chronic, diffuse, fibrocollageneous thickening of parietal and visceral peritoneum with secondary encapsulation of abdominal organs, mostly small intestines. Etiopathogenesis remains incompletely understood. SEP can be divided into primary, idiopathic forms and secondary forms. Secondary SEP can be caused by many different underlying disorders of which peritoneal dialysis is the most common one in humans. Diagnosis of SEP remains difficult due to its vague clinical symptoms; therefore, a combination of medical imaging, surgery and histopathology is warranted. Treatment is challenging and the prognosis is variable, depending on the severity of the disease. A combination of surgery, medicinal therapy, nutritional support and treatment of underlying disorders is used. In this article, two feline cases of SEP are described, followed by a review of the literature