Phytoremediation potential of alimurgic plants in metal-contaminated environments

As soil metal contamination is rapidly increasing worldwide, mainly because of human activities, phytomanagement of polluted land is becoming an environmentally sustainable and cost-effective option that can also produce biomass and energy as secondary utilities. Some Asteraceae and Polygonaceae species growing spontaneously as invasive weeds on natural and farmed land, which are considered medical or edible alimurgic species, have been identified to accumulate high above-ground levels of toxic elements, thanks to efficient root-to-shoot translocation. Most of them combine high adaptability to marginal soils with good shoot biomass, and many are metal indicator or hyperaccumulator species. In this study were investigated the shoot and root growth potential, metal uptake and translocation at the flowering stage of wild chicory (Cichorium intybus L.), common sowthistle (Sonchus oleraceus L.), salsify (Tragopogon porrifolius L.), common dandelion (Taraxacum officinale Web.) and garden sorrel (Rumex acetosa L.) in artificially highly Cd-Co-Cu-Pb-Zn-contaminated soil

Phytoremediation opportunities with alimurgic species in metal-contaminated environments

Alimurgic species are edible wild plants growing spontaneously as invasive weeds in natural grassland and farmed fields. Growing interest in biodiversity conservation projects suggests deeper study of the multifunctional roles they can play in metal uptake for phytoremediation and their food safety when cultivated in polluted land. In this study, the responses of the tap-rooted perennial species Cichorium intybus L., Sonchus oleracerus L., Taraxacum officinaleWeb., Tragopogon porrifolius L. and Rumex acetosa L. were studied in artificially-highly Cd-Co-Cu-Pb-Zn-contaminated soil in a pot-scale trial, and those of T. officinale and R. acetosa in critical open environments (i.e., landfill, ditch sediments, and sides of highly-trafficked roads). Germination was not inhibited, and all species showed appreciable growth, despite considerable increases in tissue metal rates. Substantial growth impairments were observed in C. intybus, T. officinale and T. porrifolius; R. acetosa and S. oleracerus were only marginally affected. Zn was generally well translocated and reached a high leaf concentration, especially in T. officinale (~600 mg/kg dry weight, DW), a result which can be exploited for phytoremediation purposes. The elevated Cd translocation also suggested applications to phytoextraction, particularly with C. intybus, in which leaf Cd reached ~16 mg/kg DW. The generally high root retention of Pb and Cu may allow their phytostabilisation in the medium-term in no-tillage systems, together with significant reductions in metal leaching compared with bare soil. In open systems, critical soil Pb and Zn were associated with heavily trafficked roadsides, although this was only seldom reflected in shoot metal accumulation. It is concluded that a community of alimurgic species can serve to establish an efficient, long-lasting vegetation cover applied for phytoremediation and reduction of soil metal movements in degraded environments. However, their food use is not recommended, since leaf Cd and Pb may exceed EU safety thresholds

Combined endophytic inoculants enhance nickel phytoextraction from serpentine soil in the hyperaccumulator Noccaea caerulescens

This study assesses the effects of specific bacterial endophytes on the phytoextraction capacity of the Ni-hyperaccumulator Noccaea caerulescens, spontaneously growing in a serpentine soil environment. Five metal-tolerant endophytes had already been selected for their high Ni tolerance (6 mM) and plant growth promoting ability. Here we demonstrate that individual bacterial inoculation is ineffective in enhancing Ni translocation and growth of N. caerulescens in serpentine soil, except for specific strains Ncr-1 and Ncr-8, belonging to the Arthrobacter and Microbacterium genera, which showed the highest indole acetic acid production and 1-aminocyclopropane-1-carboxylic acid-deaminase activity. Ncr-1 and Ncr-8 co-inoculation was even more efficient in promoting plant growth, soil Ni removal, and translocation of Ni, together with that of Fe, Co, and Cu. Bacteria of both strains densely colonized the root surfaces and intercellular spaces of leaf epidermal tissue. These two bacterial strains also turned out to stimulate root length, shoot biomass, and Ni uptake in Arabidopsis thaliana grown in MS agar medium supplemented with Ni. It is concluded that adaptation of N. caerulescens in highly Ni-contaminated serpentine soil can be enhanced by an integrated community of bacterial endophytes rather than by single strains; of the former, Arthrobacter and Microbacterium may be useful candidates for future phytoremediation trials in multiple metal-contaminated sites, with possible extension to non-hyperaccumulator plants

16S rDNA Profiling to Reveal the Influence of Seed-Applied Biostimulants on the Rhizosphere of Young Maize Plants

In an open field trial on two agricultural soils in NW Italy, the impact of two seed-applied biostimulants on the rhizosphere bacterial community of young maize plants was evaluated. The 16S rDNA profiling was carried out on control and treated plant rhizosphere samples collected at the 4-leaf stage and on bulk soil. In both soils, the rhizospheres were significantly enriched in Proteobacteria, Actinobacteria, and Bacteriodetes, while the abundances of Acidobacteria, Cloroflexi and Gemmatimonadetes decreased compared with bulk soil. Among the culturable bacteria genera that showed an increase by both biostimulants, most are known to be beneficial for nutrient uptake, such as Opitutus, Chryseolinea, Terrimonas, Rhodovastum, Cohnella, Pseudoduganella and the species Anaeromyxobacter dehalogenans; others are known to be involved in root growth, such as Niastella, Labrys, Chloroflexia and Thermomonas; or in plant defence, such as Ohtaekwangia, Quadrisphaera, Turneriella, and Actinoallomurus. Both biostimulants were also found to stimulate gen. Nannocystis, a potential biocompetitive agent against aflatoxigenic Aspergillus moulds. Under controlled conditions, both biostimulants enhanced the shoot and root biomass at the 4\u20135 leaf stage. We conclude that the biostimulants do not decrease the biodiversity of the microbial community rhizosphere of young maize plants, but stimulate rare bacterial taxa, some involved in plant growth and pathogen resistance, a result that may have implications in improving crop management

Adaptability and Sustainable Management of High-Erucic Brassicaceae in Mediterranean Environment

none4The use of high-erucic acid oils is currently receiving increasing attention, due to the great interest in chemical compounds derived from “green feedstock”. At world level, the production of these raw materials is constantly growing, and a real niche market has progressively been created. This scenario will allow greater substitution of chemicals with “green” compounds, and the introduction of industrial oilcrops. may lead to further expansion of the green market. Alternative uses of crops for nonfood purposes may be an interesting source of profit for farmers, as is happening for higherucic acid oils. Erucic acid is an unsaturated fatty acid (C22:1) with a large number of applications in the chemical industry because it confers desirable technological characteristics, such as high lubricity, cold stability and fire resistance, on oils and derived compounds.mixedFederica Zanetti; Giuliano Mosca; Enrico Rampin; Teofilo VameraliZanetti, Federica; Mosca, Giuliano; Enrico, Rampin; Vamerali, Teofil

Biostimulant Effects of Seed-Applied Sedaxane Fungicide: Morphological and Physiological Changes in Maize Seedlings

Most crops are routinely protected against seed-born and soil-borne fungal pathogens through seed-applied fungicides. The recently released succinate dehydrogenase inhibitor (SDHI), sedaxane\uae, is a broad-spectrum fungicide, used particularly to control Rhizoctonia spp., but also has documented growth-enhancement effects on wheat. This study investigates the potential biostimulant effects of sedaxane and related physiological changes in disease-free maize seedlings (3-leaf stage) at increasing application doses (25, 75 and 150 \u3bcg a.i. seed-1) under controlled sterilized conditions. We show sedaxane to have significant auxin-like and gibberellin-like effects, which effect marked morphological and physiological changes according to an approximate saturation dose-response model. Maximum benefits were attained at the intermediate dose, which significantly increased root length (+60%), area (+45%) and forks (+51%), and reduced root diameter as compared to untreated controls. Sedaxane enhanced leaf and root glutamine synthetase (GS) activity resulting in greater protein accumulation, particularly in the above-ground compartment, while glutamate synthase (GOGAT) activity remained almost unchanged. Sedaxane also improved leaf phenylalanine ammonia-lyase (PAL) activity, which may be responsible for the increase in shoot antioxidant activity (phenolic acids), mainly represented by p-coumaric and caffeic acids. We conclude that, in addition to its protective effect, sedaxane can facilitate root establishment and intensify nitrogen and phenylpropanoid metabolism in young maize plants, and may be beneficial in overcoming biotic and abiotic stresses in early growth stages

Effects of field inoculation with VAM and bacteria consortia on root growth and nutrients uptake in common wheat

This study investigated the effects of a commercial biofertilizer containing the mycorrhizal fungus Rhizophagus irregularis and the diazotrophic N-fixing bacterium Azotobacter vinelandii on root and shoot growth, yield, and nutrient uptake in common wheat (Triticum aestivum L.) in order to improve the sustainable cultivation of this widespread crop. The trials were carried out in controlled conditions (rhizoboxes) and in open fields over two years to investigate the interaction between inoculation and three doses of nitrogen fertilization (160, 120 and 80 kg ha1) in a silty-loam soil of the Po Plain (NE Italy). In rhizoboxes, efficient root colonization by R. irregularis was observed at 50 days after sowing with seed inoculation, together with improved root tip density and branching (+~30% vs. controls), while the effects of post-emergence inoculation by soil and foliar spraying were not observable at plant sampling. In the open, field spraying at end tillering significantly increased the volumetric root length density (RLD, +22% vs. controls) and root area density (+18%) after about two months (flowering stage) in both years under medium and high N fertilization doses, but not at the lowest N dose. In absence of inoculation, RLD progressively decreased with increased N doses. Inoculation had a negligible effect on grain yield and N uptake, which followed a typical N dose-response model, while straw Zn, P, and K concentrations were seldom improved. It is concluded that medium-high N fertilization doses are required to achieve the target yield and standards of quality (protein contents) in wheat cultivation, while the use of this mixed VAM-PGPR biofertilizer appears to be a sustainable mean for minimizing the adverse effects of chemical N fertilizers on root expansion and for improving the uptake of low-mobility nutrients, which has potentially relevant environmental benefits

Biostimulant Effects of Seed-Applied Sedaxane Fungicide: Morphological and Physiological Changes in Maize Seedlings

Most crops are routinely protected against seed-born and soil-borne fungal pathogens through seed-applied fungicides. The recently released succinate dehydrogenase inhibitor (SDHI), sedaxane®, is a broad-spectrum fungicide, used particularly to control Rhizoctonia spp., but also has documented growth-enhancement effects on wheat. This study investigates the potential biostimulant effects of sedaxane and related physiological changes in disease-free maize seedlings (3-leaf stage) at increasing application doses (25, 75 and 150 μg a.i. seed-1) under controlled sterilized conditions. We show sedaxane to have significant auxin-like and gibberellin-like effects, which effect marked morphological and physiological changes according to an approximate saturation dose-response model. Maximum benefits were attained at the intermediate dose, which significantly increased root length (+60%), area (+45%) and forks (+51%), and reduced root diameter as compared to untreated controls. Sedaxane enhanced leaf and root glutamine synthetase (GS) activity resulting in greater protein accumulation, particularly in the above-ground compartment, while glutamate synthase (GOGAT) activity remained almost unchanged. Sedaxane also improved leaf phenylalanine ammonia-lyase (PAL) activity, which may be responsible for the increase in shoot antioxidant activity (phenolic acids), mainly represented by p-coumaric and caffeic acids. We conclude that, in addition to its protective effect, sedaxane can facilitate root establishment and intensify nitrogen and phenylpropanoid metabolism in young maize plants, and may be beneficial in overcoming biotic and abiotic stresses in early growth stages

Variations in yield and gluten proteins in durum wheat varieties under late-season foliar versus soil application of nitrogen fertilizer in a northern Mediterranean environment

BACKGROUND: With the increasing demand for high-quality foodstuffs and concern for environmental sustainability, late-season nitrogen (N) foliar fertilization of common wheat is now an important and widespread practice. This study investigated the effects of late-season foliar versus soil N fertilization on yield and protein content of four varieties of durum wheat, Aureo, Ariosto, Biensur and Liberdur, in a three-year field trial in northern Italy. RESULTS: Variations in low-molecular-weight glutenins (LMW-GS), high-molecular-weight glutenins (HMW-GS) and gliadins were assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It was found that N applied to the canopy did not improve protein rate compared with N application to the soil (general mean 138mg g 121), but moderately increased productivity in the high-yielding varieties Liberdur and Biensur (three-year means 7.23 vs 7.13 and 7.53 vs 7.09 t ha 121 respectively). Technological quality was mainly related to variety choice, Aureo and Ariosto having higher protein rates and glutenin/gliadin ratios. Also found was a strong \u2018variety 7 N application method\u2019 interaction in the proportions of protein subunits within each class, particularly LMW-GS and gliadins. A promising result was the higher N uptake efficiency, although as apparent balance, combined with higher HMW/LMW-GS ratio in var. Biensur. CONCLUSION: Late-season foliar N fertilization allows N fertilizer saving, potentially providing environmental benefits in the rainy climate of the northern Mediterranean area, and also leads to variety-dependent up-regulation of essential LMW-GS and gliadins. Variety choice is a key factor in obtaining high technological quality, although it is currently associated with modest grain yield. This study provides evidence of high quality in the specific high-yielding variety Biensur, suggesting its potential as a mono-varietal semolina for pasta production