Artrosis postraumática en pacientes con fractura-luxacion de Lisfranc.

La artrosis postraumática es una de las complicaciones más frecuentes y más invalidantes de la fractura-luxación de Lisfranc (FLL). Objetivo . Evaluar el grado de incidencia de artrosis postraumática como complicación y secuela en pacientes con fractura-luxación de Lisfranc y valorar qué factores muestran relación directa o influyen en su aparición y desarrollo. Material y Método . Se realizó un estudio de cohortes ambispectivo transversal de 83 pacientes con diagnóstico de fractura-luxación de Lisfranc. Se utilizó un protocolo de recogida de datos con variables relacionadas con la lesión, parámetros clínicos, terapéuticos, complicaciones y escalas de valoración clínica. La valoración de artrosis se realiza mediante aparición de signos radiológicos de la clasifica - ción radiológica de Kellgren y Lawrence y su correlación clínica con presencia de dolor. Resultados . La artrosis se mostró como un factor influyente en los resultados, puntuaciones regulares y malas presentan mayor tasa de artrosis (p=0,001), y en cuanto al grado de artrosis, los casos más graves de artrosis se situaban en el grupo de puntuaciones malas (p=0,025). Este grupo de pacientes presentaron peores puntuaciones porque referían más dolor, mayor consumo de analgésicos, más limitación de las actividades de la vida diaria, de las actividades deportivas y limitación de la distancia caminada. Conclusiones . La artrosis se mostró como un factor influyente en los resultados (p=0,001). Los pacientes que desarrollaron artrosis tardaron más tiempo en reincorporarse a su empleo o actividad laboral previa, tenían un tiempo más demorado desde la cirugía al alta y mayor número total de incapacidades laborales y compensaciones económicas con respecto a los pacientes que no desarrollaron artrosis, siendo todos estos valores estadísticamente significativos.The posttraumatic degenerative osteoarthritis is one of the most frequent complications and more disabling of Lisfranc's fracture - luxation (FLL). Objetive. Evaluate the degree of frequency of posttraumatic degenerative osteoarthritis as complication and adverse effect in patients with Lisfranc's fracture - luxation and value what factors show straight relation or influence in its appearance and development. Material and Method.We have made a transversal prospective and retrospective study of cohorts on 83 patients with diagnosis of Lisfranc's fracture - luxation. We have used a data collecting protocol of items related to the injury, clinical, therapeutic parameters, complications and scales of clinical valuation. The assessment valuation of degenerative osteoarthritis is made under radiological signs realized by means of appearance of radiological signs of Kellgren and Lawrence classification and clinical correlation with presence of pain. Results . The degenerative osteoarthri tis appeared as an influential factor in the results, regular and bad punctuations present major rate of degenerative osteoarthritis (p=0,001), and as for the degree of degenerative osteoarthritis, the most serious cases of degenerative osteoarthritis were placing in the group of bad punctuations (p=0,025). This group of patients presented worse punctuations because they were recounting more pain, major consumption of analgesics, more limitation of the activities of the daily life, of the sports activities and limitation of the travelled distance. Conclusions . The degenerative osteoarthritis appeared as an influential factor in the results (p=0,001). The patients who developed degenerative osteoarthritis were late more time in coming back to his employment or labor previous activity; they had a time more delayed from the surgery to the discharge and major total number of labor disabilities and economic compensations with regard to the patients who did not develop degenerative osteoarthritis, being all these statistically significant values

Discovering the Legacy of Hispanic/Spanish and South American Landscapes through Geohistorical Sources: The Geographical and Topographical Relations of Philip II

Landscapes have history and memory, which are eloquent generators of testimonies and traces on the processes of the landscape that take place today, and that will take place in the future. In recent years, numerous methods of analysing land and landscape patterns have been developed and evaluated, based on the multiplicity of these type of geographic and historical data sources, which have developed the concept of the geohistorical source. The goal of these sources of information allows us to historically reconstruct landscapes. With this in mind, the basic objective of the present research is to approach a geohistorical source with a wide spatial spectrum in Europe and America: the geographical and topographical relations of Philip II. This source has been chosen for the quality, quantity, variety and systematization of the data it provides on the territory and landscape of the crown of Castile. In addition, it ended up being the model of how to obtain organized and homogeneous knowledge of a large spatial area, considering the geographical, anthropological and historical data of the different territories. This geohistorical source is reliable, because the local authorities, both secular and ecclesiastical, are questioned, as they are the ones who inhabit, use, and, at different levels, govern the territory and its people

The correlation between immune subtypes and consensus molecular subtypes in colorectal cancer identifies novel tumour microenvironment profiles, with prognostic and therapeutic implications

Background Solid tumour growth is the consequence of a complex interplay between cancer cells and their microenvironment. Recently, a new global transcriptomic immune classification of solid tumours has identified six immune subtypes (ISs) (C1–C6). Our aim was to specifically characterise ISs in colorectal cancer (CRC) and assess their interplay with the consensus molecular subtypes (CMSs). Methods Clinical and molecular information, including CMSs and ISs, were obtained from The Cancer Genome Atlas (TCGA) (N = 625). Immune cell populations, differential gene expression and gene set enrichment analysis were performed to characterise ISs in the global CRC population by using CMSs. Results Only 5 ISs were identified in CRC, predominantly C1 wound healing (77%) and C2 IFN-γ dominant (17%). CMS1 showed the highest proportion of C2 (53%), whereas C1 was particularly dominant in CMS2 (91%). CMS3 had the highest representation of C3 inflammatory (7%) and C4 lymphocyte depleted ISs (4%), whereas all C6 TGF-β dominant cases belonged to CMS4 (2.3%). Prognostic relevance of ISs in CRC substantially differed from that reported for the global TCGA, and ISs had a greater ability to stratify the prognosis of CRC patients than CMS classification. C2 had higher densities of CD8, CD4 activated, follicular helper T cells, regulatory T cells and neutrophils and the highest M1/M2 polarisation. C2 had a heightened activation of pathways related to the immune system, apoptosis and DNA repair, mTOR signalling and oxidative phosphorylation, whereas C1 was more dependent of metabolic pathways. Conclusions The correlation of IS and CMS allows a more precise categorisation of patients with relevant clinical and biological implications, which may be valuable tools to improve tailored therapeutic interventions in CRC patients.This work was funded by projects DTS15/00157 , PI16/01827 and CIBER-ONC CB16/12/00442 from the Instituto de Salud Carlos III ( Ministry of Economy, Industry and Competitiveness, Spain ) and cofunded by the European Regional Development Fund (ERDF, European Union), and approved by the Ethics Committee or our Institution. BS is funded by AECC (Spain). MCR is funded by Instituto de Salud Carlos III and SEOM (Spain) CCP and BRC are funded by CAM (Programa de Empleo Juvenil (YEI)

O discurso dos #ProtestosBR: análise de conteúdo do Twitter

This paper focus on an analysis of the discourse of Twitter messages during the protests in Brazil during June 2013. Our objective is to discuss how the concepts relate to each other and form networks of meanings around the tweets about the protests. Our search for common characteristics and elements reveals how Twitter was used to describe the protests and to mobilize people rather than to discuss what was actually taking place. We describe the key actors, contexts and demands, as well as the focus on live narratives, #pamphleteer hashtags, and the specific location of tweets

Major role of nutrient supply in the control of picophytoplankton community structure.

abstractThe Margalef´s mandala (1978) is a simplified bottom-up control model that explains how mixing and nutrient concentration determine the composition of marine phytoplankton communities. Due to the difficulties of measuring turbulence in the field, previous attempts to verify this model have applied different proxies for nutrient supply, and very often used interchangeably the terms mixing and stratification. Moreover, because the mandala was conceived before the discovery of smaller phytoplankton groups (picoplankton <2 μm), it describes only the succession of vegetative phases of microplankton. In order to test the applicability of the classical mandala to picoplankton groups, we used a multidisciplinary approach including specifically designed field observations supported by remote sensing, database analyses, and modeling and laboratory chemostat experiments. Simultaneous estimates of nitrate diffusive fluxes, derived from microturbulence observations, and picoplankton abundance collected in more than 200 stations, spanning widely different hydrographic regimes, showed that the contribution of eukaryotes to picoautotrophic biomass increases with nutrient supply, whereas that of picocyanobacteria shows the opposite trend. These findings were supported by laboratory and modeling chemostat experiments that reproduced the competitive dynamics between picoeukaryote sand picocyanobacteria as a function of changing nutrient supply. Our results indicate that nutrient supply controls the distribution of picoplankton functional groups in the ocean, further supporting the model proposed by Margalef.RADIALES (IEO

Control of tHe structure of marine phytoplAnkton cOmmunities by turbulence and nutrient supply dynamicS (CHAOS)

extended abstract del posterIn order to investigate the role of turbulence mixing on structuring marine phytoplankton communities, the CHAOS project included a multidisciplinary approach involving specifically designed field observations supported by remote sensing, database analyses, and modeling and laboratory chemostat experiments. Field observations carried out in the outer part of Ría de Vigo in summer 2013 showed that, as a result of increased mixing levels, nitrate diffusive input into the euphotic layer was approximately 4-fold higher during spring tides. This nitrate supply could contribute to explain the continuous dominance of large-sized phytoplankton during the upwelling favorable season. Simultaneous estimates of nitrate diffusive fluxes, derived from microturbulence observations, and picoplankton abundance collected in more than 100 stations, spanning widely different hydrographic regimes, showed that the contribution of eukaryotes to picoautotrophic biomass increases with nutrient supply, whereas that of picocyanobacteria shows the opposite trend. These findings were supported by laboratory and modeling chemostat experiments that reproduced the competitive dynamics between picoeukaryote and picocyanobacteria as a function of changing nutrient supply. The results derived from this project confirm that turbulence and mixing control the availability of light and nutrients, which in turn determine the structure of marine phytoplankton communities.RADIALES-20 (IEO), CHAOS (CTM 2012-30680), Malaspina-2010(CSD2008-00077

Nutrient supply does play a role on the structure of marine picophytoplankton communities

Conference communicationThe Margalef´s mandala (1978) is a simplified bottom-up control model that explains how mixing and nutrient concentration determine the composition of marine phytoplankton communities. Due to the difficulties of measuring turbulence in the field, previous attempts to verify this model have applied different proxies for nutrient supply, and very often used interchangeably the terms mixing and stratification. Moreover, because the mandala was conceived before the discovery of smaller phytoplankton groups (picoplankton <2 µm), it describes only the succession of vegetative phases of microplankton. In order to test the applicability of the classical mandala to picoplankton groups, we used a multidisciplinary approach including specifically designed field observations supported by remote sensing, database analyses, and modeling and laboratory chemostat experiments. Simultaneous estimates of nitrate diffusive fluxes, derived from microturbulence observations, and picoplankton abundance collected in more than 200 stations, spanning widely different hydrographic regimes, showed that the contribution of eukaryotes to picoautotrophic biomass increases with nutrient supply, whereas that of picocyanobacteria shows the opposite trend. These findings were supported by laboratory and modeling chemostat experiments that reproduced the competitive dynamics between picoeukaryote sand picocyanobacteria as a function of changing nutrient supply. Our results indicate that nutrient supply controls the distribution of picoplankton functional groups in the ocean, further supporting the model proposed by Margalef.Spanish Governmen

Adaptive data acquisition strategies for energy-efficient, smartphone-based, continuous processing of sensor streams

Ministry of Education, Singapore under its Academic Research Funding Tier

Methanethiol-dependent dimethylsulfide production in soil environments

Dimethylsulfide (DMS) is an environmentally important trace gas with roles in sulfur cycling, signalling to higher organisms and in atmospheric chemistry. DMS is believed to be predominantly produced in marine environments via microbial degradation of the osmolyte dimethylsulfoniopropionate (DMSP). However, significant amounts of DMS are also generated from terrestrial environments, for example, peat bogs can emit ~6 μmol DMS m−2 per day, likely via the methylation of methanethiol (MeSH). A methyltransferase enzyme termed ‘MddA’, which catalyses the methylation of MeSH, generating DMS, in a wide range of bacteria and some cyanobacteria, may mediate this process, as the mddA gene is abundant in terrestrial metagenomes. This is the first study investigating the functionality of MeSH-dependent DMS production (Mdd) in a wide range of aerobic environments. All soils and marine sediment samples tested produced DMS when incubated with MeSH. Cultivation-dependent and cultivation-independent methods were used to assess microbial community changes in response to MeSH addition in a grassland soil where 35.9% of the bacteria were predicted to contain mddA. Bacteria of the genus Methylotenera were enriched in the presence of MeSH. Furthermore, many novel Mdd+ bacterial strains were isolated. Despite the abundance of mddA in the grassland soil, the Mdd pathway may not be a significant source of DMS in this environment as MeSH addition was required to detect DMS at only very low conversion rates

In Vivo Tumor Targeting and Imaging with Engineered Trivalent Antibody Fragments Containing Collagen-Derived Sequences

There is an urgent need to develop new and effective agents for cancer targeting. In this work, a multivalent antibody is characterized in vivo in living animals. The antibody, termed “trimerbody”, comprises a single-chain antibody (scFv) fragment connected to the N-terminal trimerization subdomain of collagen XVIII NC1 by a flexible linker. As indicated by computer graphic modeling, the trimerbody has a tripod-shaped structure with three highly flexible scFv heads radially outward oriented. Trimerbodies are trimeric in solution and exhibited multivalent binding, which provides them with at least a 100-fold increase in functional affinity than the monovalent scFv. Our results also demonstrate the feasibility of producing functional bispecific trimerbodies, which concurrently bind two different ligands. A trimerbody specific for the carcinoembryonic antigen (CEA), a classic tumor-associated antigen, showed efficient tumor targeting after systemic administration in mice bearing CEA-positive tumors. Importantly, a trimerbody that recognizes an angiogenesis-associated laminin epitope, showed excellent tumor localization in several cancer types, including fibrosarcomas and carcinomas. These results illustrate the potential of this new antibody format for imaging and therapeutic applications, and suggest that some laminin epitopes might be universal targets for cancer targeting