Response of osteoblasts and preosteoblasts to calcium deficient and Sisubstituted hydroxyapatites treated at different temperatures

tHydroxyapatite (HA) is a calcium phosphate bioceramic widely used for bone grafting and augmenta-tion purposes. The biological response of HA can be improved through chemical and microstructuralmodifications, as well as by manufacturing it as macroporous implants. In the present study, calciumdeficient hydroxyapatite (CDHA) and Si substituted hydroxyapatite (SiHA) macroporous scaffolds havebeen prepared by robocasting. In order to obtain different microstructural properties, the scaffolds havebeen treated at 700◦C and 1250◦C. The scaffolds have been characterized and tested as supports forboth osteoblast growth and pre-osteoblast differentiation, as fundamental requisite for their potentialuse in bone tissue engineering. Morphology, viability, adhesion, proliferation, cell cycle, apoptosis, intra-cellular content of reactive oxygen species and interleukin-6 production were evaluated after contactof osteoblasts-like cells with CDHA and SiHA materials. An adequate interaction of osteoblasts-like cellsand preosteoblasts-like cells with all these scaffolds was observed. However, the higher bone cell pro-liferation and differentiation on CDHA and SiHA scaffolds treated at 1250◦C and the lower adsorptionof albumin and fibrinogen on these materials in comparison to those treated at 700◦C, suggest a bettertissue response to CDHA and SiHA materials treated at high temperature

In Vitro and In Vivo Response of Zinc-Containing Mesoporous Bioactive Glasses in a Sheep Animal Model

Zinc-enriched mesoporous bioactive glasses (MBGs) are bioceramics with potential antibacterial and osteogenic properties. However, few assays have been performed to study these properties in animal models.In this study, MBGs enriched with up to 5% ZnO were synthesized, physicochemically characterized, and evaluated for their osteogenic activity both in vitro and in vivo. The ZnO MBGs showed excellent textural properties despite ZnO incorporation. However, the release of Zn2+ ions inhibited the mineralization process when immersed in simulated body fluid. In vitro assays showed significantly highe r values of viability and expression of early markers of celldifferentiation and angiogenesis in a ZnO-content-dependent manner. The next step was to study the osteogenic potential in a sheep bone defect model. Despite their excellent textural properties and cellular response in vitro, the ZnO MBGs were not able to integrate into the bone tissue, which can be explained in terms of inhibition of the mineralization process caused by Zn2+ ions. This work highlights the need to develop nanostructured materials for bone regeneration that can mineralize to interact with bone tissue and induce the processes of implant acceptance, cell colonization by osteogenic cells, and regeneration of lost bone tissue

La enseñanza en el máster de abogacía a través del análisis de casos reales de carácter multidisciplinar. Aprendizaje "a partir del estudio de razonamientos parciales temáticos"

Generar blog para publicaciones de alumnos del Máster Abogacía, con "entradas" dirigidas. Habría análisis desde varias disciplinas añadiéndose estudios con enfoque basado en otras áreas de conocimiento, generando interdisciplinariedad