Magnetic fields at the periphery of UCHII regions from carbon recombination line observations

Several indirect evidences indicate a magnetic origin for the non-thermal width of spectral lines observed toward molecular clouds. In this letter, I suggest that the origin of the non-thermal width of carbon recombination lines (CRLs) observed from photo-dissociation regions (PDRs) near ultra-compact \HII\ regions is magnetic and that the magnitude of the line width is an estimate of the \alfven speed. The magnetic field strengths estimated based on this suggestion compare well with those measured toward molecular clouds with densities similar to PDR densities. I conclude that multi-frequency CRL observations have the potential to form a new tool to determine the field strength near star forming regions.Comment: To appear in ApJ Letter

Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages

Microcephaly is a cortical malformation disorder characterized by an abnormally small brain. Recent studies have revealed severe cases of microcephaly resulting from human mutations in the NDE1 gene, which is involved in the regulation of cytoplasmic dynein. Here using in utero electroporation of NDE1 short hairpin RNA (shRNA) in embryonic rat brains, we observe cell cycle arrest of proliferating neural progenitors at three distinct stages: during apical interkinetic nuclear migration, at the G2-to-M transition and in regulation of primary cilia at the G1-to-S transition. RNAi against the NDE1 paralogue NDEL1 has no such effects. However, NDEL1 overexpression can functionally compensate for NDE1, except at the G2-to-M transition, revealing a unique NDE1 role. In contrast, NDE1 and NDEL1 RNAi have comparable effects on postmitotic neuronal migration. These results reveal that the severity of NDE1-associated microcephaly results not from defects in mitosis, but rather the inability of neural progenitors to ever reach this stage

NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores

NudE and NudEL are related proteins that interact with cytoplasmic dynein and LIS1. Their functional relationship and involvement in LIS1 and dynein regulation are not completely understood. We find that NudE and NudEL each localize to mitotic kinetochores before dynein, dynactin, ZW10, and LIS1 and exhibit additional temporal and spatial differences in distribution from the motor protein. Inhibition of NudE and NudEL caused metaphase arrest with misoriented chromosomes and defective microtubule attachment. Dynein and dynactin were both displaced from kinetochores by the injection of an anti-NudE/NudEL antibody. Dynein but not dynactin interacted with NudE surprisingly through the dynein intermediate and light chains but not the motor domain. Together, these results identify a common function for NudE and NudEL in mitotic progression and identify an alternative mechanism for dynein recruitment to and regulation at kinetochores

The Magnetic Field Geometry in M82 and Cen A

Imaging polarimetry at 1.65 and 2.2 $\mu$m is presented for the classic starburst galaxy M82 and the advanced merger system Cen A. Polarimetry at near IR wavelengths allows the magnetic field geometry in galaxies to be probed much deeper into dusty regions than optical polarimetry. In M82, the magnetic field throughout the nucleus has a polar geometry, presumably due to the massive vertical flow that is a result of the intense star formation there. Fully two thirds of the line of sight dust through to the center of M82 contains a vertical magnetic field. In Cen A, the prominent dust lane shows a normal planar field geometry. There is no indication of significant disturbance in the field geometry in the dust lane and the polarization strength is near normal for the amount of extinction. Either the magnetic field geometry was well maintained during the merger, or it reestablished itself very easily