Raman Tweezers Spectroscopy of Live, Single Red and White Blood Cells

An optical trap has been combined with a Raman spectrometer to make high-resolution measurements of Raman spectra of optically-immobilized, single, live red (RBC) and white blood cells (WBC) under physiological conditions. Tightly-focused, near infrared wavelength light (1064 nm) is utilized for trapping of single cells and 785 nm light is used for Raman excitation at low levels of incident power (few mW). Raman spectra of RBC recorded using this high-sensitivity, dual-wavelength apparatus has enabled identification of several additional lines; the hitherto-unreported lines originate purely from hemoglobin molecules. Raman spectra of single granulocytes and lymphocytes are interpreted on the basis of standard protein and nucleic acid vibrational spectroscopy data. The richness of the measured spectrum illustrates that Raman studies of live cells in suspension are more informative than conventional micro-Raman studies where the cells are chemically bound to a glass cover slip

Ovarian Pregnancy following Intracytoplasmic Sperm Injection and Embryo Transfer: A Case Report

Ovarian pregnancy is a rare form of ectopic pregnancy following ICSI-ET, and the diagnosis depends on the physicians suspicion and experience. Preservation of ovarian tissue during surgery is of utmost importance to preserve fertility. We present a case of ovarian pregnancy who had a successful treatment preserving the ovary

Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml

Optical pathology of oral tissue: a Raman spectroscopy diagnostic method

Raman and fluorescence spectroscopy methods are being considered as techniques which could be complementary or even alternative to biopsy, and pathology and clinical assays in many medical applications. The present paper discusses the results of Raman spectral studies on oral tissues for optical pathology. It is shown that Raman spectra of oral tissues can be classified into spectra of normal and malignant sets and a model based on such a classification can be used to analyse oral tissue for detection of oral malignancy. Sensitivity and specificity calculated from 90 test spectra are better than 85 and 90 per cent respectively

Adenomatoid Tumours: A Short Series with Literature Review

Adenomatoid tumours are hormone independent tumours of mesothelial origin. These are distinctive benign neoplasms usually localised in the epididymis in males and myometrium in females. They are uncommon and account for less than 5% of tumours in males and approximately 1% in females. Due to their rarity and distinct histomorphology, we present a case series of six adenomatoid tumours of which three were males and three were females, obtained over a period of five years at a tertiary hospital in South India. The diagnosis of this tumour in all the three women and in one of the male patients was incidental, and they underwent surgical procedure for other coexisting lesions. In two male patients swelling of the testis was present; however, the clinical diagnosis of both these patients was more sinister than a benign entity like adenomatoid tumour. The present series describe the histomorphological features of this entity and establish the unique histopathological findings of this tumour which is clinically subtle and is seldom recognised