A star is born again: Methods for larval rearing of an emerging model organism, the False clownfish Amphiprion ocellaris

As interest increases in ecological, evolutionary, and developmental biology (Eco-Evo-Devo), wild species are increasingly used as experimental models. However, we are still lacking a suitable model for marine fish species, as well as coral reef fishes that can be reared at laboratory scales. Extensive knowledge of the life cycle of anemonefishes, and the peculiarities of their biology, make them relevant marine fish models for developmental biology, ecology, and evolutionary sciences. Here, we present standard methods to maintain breeding pairs of the anemonefish Amphiprion ocellaris in captivity, obtain regular good quality spawning, and protocols to ensure larval survival throughout rearing. We provide a detailed description of the anemonefish husbandry system and life prey culturing protocols. Finally, a “low-volume” rearing protocol useful for the pharmacological treatment of larvae is presented. Such methods are important as strict requirements for large volumes in rearing tanks often inhibit continuous treatments with expensive or rare compounds

Exposure to four chemical UV filters through contaminated sediment: impact on survival, hatching success, cardiac frequency and aerobic metabolic scope in embryo-larval stage of zebrafish

International audienc

Impact of Egg Exposure to UV Filter-Spiked Sediment on the Survival, Hatching Success, Cardiac Frequency and Metabolic Scope of Zebrafish Embryos

International audienceChemical UV filters are increasingly used in cosmetics to protect skin from UV radiation. As a consequence, they are released into the aquatic environment via recreational activities and wastewaters. In aquatic ecosystems, fish eggs in contact with sediment can be affected by organic and lipophilic pollutants such as UV filters. The present study aims to evaluate the toxicity of six individual UV filters, diethylhexyl butamido triazone (DBT), diethylamino hydroxybenzoyl hexyl benzoate (DHHB), ethylhexyl triazone (ET), 2-ethylhexyl salicylate (ES), homosalate (HS), and octocrylene (OC), in embryo-larval stages of zebrafish Danio rerio. Contamination of fish eggs and larvae with UV filters occurred by contact with spiked sediment for 96 h at a concentration of 10 μg g −1. Among the six UV filters tested, OC delayed hatching success whereas ES significantly increased the heartbeat rate of embryo-larvae after sediment exposure probably as a stress response