Wireless sensor network for ignitions detection: an IoT approach

Wireless Sensor Networks (WSN) can be used to acquire environmental variables useful for decision-making, such as agriculture and forestry. Installing a WSN on the forest will allow the acquisition of ecological variables of high importance on risk analysis and fire detection. The presented paper addresses two types of WSN developed modules that can be used on the forest to detect fire ignitions using LoRaWAN to establish the communication between the nodes and a central system. The collaboration between these modules generate a heterogeneous WSN; for this reason, both are designed to complement each other. The first module, the HTW, has sensors that acquire data on a wide scale in the target region, such as air temperature and humidity, solar radiation, barometric pressure, among others (can be expanded). The second, the 5FTH, has a set of sensors with point data acquisition, such as flame ignition, humidity, and temperature. To test HTW and 5FTH, a LoRaWAN communication based on the Lorix One gateway is used, demonstrating the acquisition and transmission of forest data (simulation and real cases). Even in internal or external environments, these results allow validating the developed modules. Therefore, they can assist authorities in fighting wildfire and forest surveillance systems in decision-making.This work is financed by SAFe Project through PROMOVE—Fundação La Caixainfo:eu-repo/semantics/publishedVersio

Natural attenuation processes in AMD context by mineralogical control: iron oxyhydroxides, oxyhydroxysulfates, and efflorescent sulfates

Acid mine drainage (AMD) is a common problem associated with the weathering of metal sulfide wastes in abandoned mining areas, which can be rich in toxic metals and metalloids. Oxidative dissolution of these minerals may negatively affect the soils and water reservoirs nearby. The formation of secondary mineral phases such as iron oxyhydroxides, oxyhydroxysulfates, and efflorescent sulfates may be a natural attenuation process in these areas because these minerals can scavenge potentially toxic elements. Typically formed by the evaporation (efflorescent salts) or precipitation (ochreous iron-rich oxyhydroxides) these minerals are highly soluble and reactive. Therefore, their characterization and inventory are particularly important for predicting the cycles of retention/contamination or accumulation zones of potential toxic elements (PTE) and acidity in mining contaminated systems. The abandoned mines of Valdarcas and São Domingos were selected for the present study due to their AMD signature and occurrence of some of these secondary minerals. Valdarcas (north of Portugal) is a W-mine associated with a skarn deposit with sulfides that has already been rehabilitated. However, the AMD problems remain in the watercourse that receives leachates from the waste dumps. São Domingos is a Cu-mine composed of massive sulfide deposits that belong to the Iberian Pyrite Belt (south of Portugal). This mining area, which is presently under remediation process, is highly contaminated and AMD is a longlasting problem. The samples collected in these mining areas were characterized by their morphology, size, mineralogy, and chemical composition using different techniques: binocular microscopy, SEM-EDS, TEM, BET, DRX, FTIR, and XPS. In Valdarcas mine area, typical ochreous phases such as schwertmannite and goethite were identified as the dominant secondary phases. These brownish-yellow ferric amorphous and poorly crystalline minerals with high surface area (125 mg2 /m) can remove PTE via adsorption processes, contributing to their retention in the mining environment. In contrast, at São Domingos, efflorescent sulfate salts are more abundant. Melanterite and copiapite were the prevailing salts identified. With different morphologies, these minerals are highly soluble and therefore play a relevant key in the retention/mobilization cycles of hazardous contaminants, such as arsenic (As). This integrative methodologic approach, based on the use of several techniques, allowed a complete characterization of these minerals. In addition to composition, the surface properties that are relevant for the adsorption ability, were also obtained. Therefore, the study revealed a diversity of behaviors for the identified phases, suggesting various roles in increasing the resilience of the contaminated systems

Efficient extraction of the RuBisCO enzyme from spinach leaves using aqueous solutions of biocompatible ionic liquids

Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not allow high yields of extraction. Therefore, the development of an efficient and selective RuBisCOs’ extraction method is required. In this work, aqueous solutions of biocompatible ionic liquids (ILs), i.e., ILs derived from choline and analogues of glycine-betaine, were applied in the RuBisCO’s extraction from spinach leaves. Three commercial imidazolium-based ILs were also investigated for comparison purposes. To optimize RuBisCO’s extraction conditions, response surface methodology was applied. Under optimum extraction conditions, extraction yields of 10.92 and 10.57 mg of RuBisCO/g of biomass were obtained with the ILs cholinium acetate ([Ch][Ac]) and cholinium chloride ([Ch]Cl), respectively. Circular dichroism (CD) spectroscopy results show that the secondary structure of RuBisCO is better preserved in the IL solutions when compared to the commonly used extraction solvent. The obtained results indicate that cholinium-based ILs are a promising and viable alternative for the extraction of RuBisCO from vegetable biomass.publishe

Optimal sensors positioning to detect forest fire ignitions

Forests have been harassed by fire in recent years. Whether by human action or for other reasons, the burned area has increased harming fauna and flora. It is fundamental to detect an ignition early in order to firefighters fight the fire minimizing the fire impacts. The proposed Forest Monitoring System aims at improving the nature monitoring and to enhance the existing surveillance systems. A set of innovative operations is proposed that will allow to identify a forest ignition and also will monitor the fauna. For that, a set of sensors are being developed and placed in the forest to transmit data and identify forest fire ignition. This paper addresses a methodology that identifies the ideal positions to place the developed sensors in order to minimize the fire hazard. Some preliminary results are shown by a random algorithm that spread points to position sensor modules in areas with high risk of fire hazard.This work has been supported by FCT — Fundação para a Ciência e Tecnologia within the Project Scope: UIDB/5757/2020.info:eu-repo/semantics/publishedVersio

Integrated platform resorting to ionic liquids comprising the extraction, purification and preservation of DNA

The large-scale production of therapeutically targeted-deoxyribonucleic acid (DNA) has passed through several challenges, postponing the tangible implementation of an effective, economic and sustainable manufacturing system. Such challenges comprise the need to develop an integrative downstream process able to extract, purify and long-term preserve DNA, whilst reducing the risk of degradation by endonucleases that would compromise their effectiveness as therapeutic products. In this work, three-phase partitioning (TPP) systems formed by the application of aqueous biphasic systems (ABS) composed of several biocompatible cholinium-based ionic liquids (ILs), are proposed for the separation of double stranded DNA (dsDNA) from the endonuclease deoxyribonuclease I (DNase I). By taking advantage of the tailor-made properties of ILs, dsDNA can be completely extracted to the IL-rich phase, whereas DNase I is precipitated at the ABS interphase. The ABS/TPP formed by IL cholinium glycolate ([N 111(2OH) ][Gly]) fulfills the aim of this work, i.e. at ensuring the technical viability of IL-based ABS/ TPP for the “one-pot” extraction, purification and long-term preservation of dsDNA. The results reveal the potential of this system to be applied in the bioprocessing of DNA, particularly relevant when envisioning DNA- based therapeutic products.publishe

Insights on the laccase extraction and activity in ionic-liquid-based aqueous biphasic systems

Due to their catalytic properties, selectivity, and efficiency, enzymes are excellent biocatalysts. In particular, laccases are versatile multi-copper oxidases with great interest for a wide plethora of biotechnological and environmental applications. Even though several laccase-catalysed processes have been reported at an industrial level, the high costs of their downstream processing required to provide biocatalysts with high purity levels, stability and activity remains one of the main drawbacks when economically evaluating the overall processes. Aqueous biphasic systems based on ionic liquids (ILs) can be foreseen as a promising alternative approach for the extraction and activity maintenance/improvement of enzymes, essentially due to the designer solvents ability of ionic liquids. However, to take advantage of this feature and to use the full potential of IL-based aqueous biphasic systems, it is necessary to understand the effect of ILs as phase-forming constituents and how they affect the enzymes extraction and activity. In order to overcome the lack of information on this topic in the literature, in this work, IL-based aqueous biphasic systems were investigated to extract and enhance the laccase activity, in order to gather evidences that could be used to improve the enzymes downstream processing. To this end, a wide screening of imidazolium-, pyridinium-, pyrrolidinium-, piperidinium-, tetraalkylphosphonium-, and tetraalkylammonium-based ILs as phase-forming components of ABS was carried out. Furthermore, these ILs were used to create ABS combined with salts, polymers and used as adjuvants in polymer-based ABS. Most ABS comprising ILs revealed to be highly efficient extraction platforms, allowing the complete extraction of laccase for all the conditions tested, and with an enzyme activity enhancement by more than 50%. Overall, the obtained results demonstrate that laccase preferentially partitions to the most hydrophilic phase in ABS comprising ILs, both used as adjuvants or as phase-forming components, corresponding to the phase in which the IL is enriched. Furthermore, the IL chemical structure of the IL plays a significant role in the enzyme activity, where ILs with a higher number of hydroxyl groups seem to be relevant to improve the laccase activity.publishe

Smart systems for monitoring buildings - an IoT application

Life in society has initiated a search for comfort and security in social centers. This search generated revolutions within the knowledge about the technologies involved, making the environments automated and integrated. Along with this increase, ecological concerns have also arisen, which have been involved since the design of intelligent buildings, remaining through the years of their use. Based on these two pillars, the present study aims to monitor three central systems inside the apartments of the Apolo Building (Bragan¸cacity, Portugal). The electrical energy consumption, water flow, and waste disposal systems are integrated through a single database. The data is sent remotely via WiFi through the microcontroller. For better visualization and analytics of the data, a web application is also developed, which allows for real-time monitoring. The obtained results demonstrate to the consumer his behavior regarding household expenses. The idea of showing the consumer their expenditure is to create an ecological awareness. Through the data collected and the environmental alternatives found, it is possible to observe whether there was a behavior change when receiving this data, either in the short or long term.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CeDRI (UIDB/05757/2020 and UIDP/05757/2020) and SusTEC (LA/P/0007/2021). Thadeu Brito was supported by FCT PhD grant SFRH/BD/08598 /2020 and João Braun received the support of a fellowship from ”la Caixa” Foundation (ID 100010434) with code LCF/BQ/DI20/11780028.info:eu-repo/semantics/acceptedVersio

Copiapite group: occurrence and properties in mining contamination scenarios

O grupo copiapite apresentou uma ampla gama de composições químicas, permitindo a identificação de várias fases minerais (por exemplo, aluminocopiapite e magnesiocopiapite). Esses minerais, normalmente observados em ambientes mineiros, são formados a partir da precipitação e evaporação da drenagem ácida da mina. Nessas áreas, a copiapite tem sido estudada para compreender a sua capacidade de adsorção de alguns elementos potencialmente tóxicos. O presente trabalho visa caracterizar amostras minerais de copiapite, utilizando diferentes técnicas, para entender a sua morfologia, composição mineralógica e química, bem como o seu papel ambiental

Biomanipulação nas lagoas das Sete Cidades e das Furnas (São Miguel, Açores)

XII congresso da Associação Espanhola de Limnologia/IV Congresso Ibérico de Limnologia, organizado pela Associação Espanhola de Limnologia, de 5 a 9 de Julho de 2004 no Porto, Portugal.A qualidade da água de um sistema lacustre de baixa profundidade não deve ser avaliada apenas segundo a concentração de nutrientes, uma vez que esses sistemas podem existir em dois estados alternativos: um de água transparente, dominado por macrófitos, e outro de água turva dominado por fitoplâncton

Cooperative heterogeneous robots for autonomous insects trap monitoring system in a precision agriculture scenario

The recent advances in precision agriculture are due to the emergence of modern robotics systems. For instance, unmanned aerial systems (UASs) give new possibilities that advance the solution of existing problems in this area in many different aspects. The reason is due to these platforms’ ability to perform activities at varying levels of complexity. Therefore, this research presents a multiple-cooperative robot solution for UAS and unmanned ground vehicle (UGV) systems for their joint inspection of olive grove inspect traps. This work evaluated the UAS and UGV vision-based navigation based on a yellow fly trap fixed in the trees to provide visual position data using the You Only Look Once (YOLO) algorithms. The experimental setup evaluated the fuzzy control algorithm applied to the UAS to make it reach the trap efficiently. Experimental tests were conducted in a realistic simulation environment using a robot operating system (ROS) and CoppeliaSim platforms to verify the methodology’s performance, and all tests considered specific real-world environmental conditions. A search and landing algorithm based on augmented reality tag (AR-Tag) visual processing was evaluated to allow for the return and landing of the UAS to the UGV base. The outcomes obtained in this work demonstrate the robustness and feasibility of the multiple-cooperative robot architecture for UGVs and UASs applied in the olive inspection scenario.The authors would like to thank the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CeDRI (UIDB/05757/2020 and UIDP/05757/2020) and SusTEC (LA/P/0007/2021). In addition, the authors would like to thank the following Brazilian Agencies CEFET-RJ, CAPES, CNPq, and FAPERJ. In addition, the authors also want to thank the Research Centre in Digitalization and Intelligent Robotics (CeDRI), Instituto Politécnico de Braganca (IPB) - Campus de Santa Apolonia, Portugal, Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Portugal, INESC Technology and Science - Porto, Portugal and Universidade de Trás-os-Montes e Alto Douro - Vila Real, Portugal. This work was carried out under the Project “OleaChain: Competências para a sustentabilidade e inovação da cadeia de valor do olival tradicional no Norte Interior de Portugal” (NORTE-06-3559-FSE-000188), an operation used to hire highly qualified human resources, funded by NORTE 2020 through the European Social Fund (ESF).info:eu-repo/semantics/publishedVersio