Rehabilitación de cabañas pasiegas. Cabañas con encanto.

Se expone la rehabilitación de unas cabañas que se encuentran en el valle del Pas, antiguas construcciones cuya finalidad era guardar el ganado. Actualmente estas construcciones se encuentran abandonadas y tras su rehabilitación su uso es de complejo residencial-hotelero destinado a relanzar el turismo rural y la repoblación de pueblos abandonados. Se da a conocer la historia de estas construcciones y se analizan posibles soluciones para los problemas que se plantean actualmente en el medio rural. El fin es recopilar una documentación para dar a conocer en que consiste la rehabilitación de las cabañas pasiegas mostrando paso a paso su evolución con el fin de dar a conocer el entorno y como estas cabañas colaboran en la repoblación de los pueblos abandonados. Actualmente existen 10 cabañas rehabilitadas pero el trabajo se centrará especialmente en la rehabilitación de la última cabaña que se está reformando en la actualidad, Cabaña “La Brena”, con el fin de mostrar de primera mano las fases por las que trascurre la rehabilitación. El trabajo no comprenderá la rehabilitación completa de la cabaña puesto que por motivos sanitarios la rehabilitación vio obligada a detenerse durante 3 meses por un confinamiento a nivel nacional. Es por eso que el trabajo comprenderá la rehabilitación de la cabaña hasta la fecha de entrega del mismo y el resto de la rehabilitación será documentada a partir de cabañas ya rehabilitadas.Grado en Fundamentos de la Arquitectur

Exploring cryptic amyloidogenic regions in prion-like proteins from plants

Prion-like domains (PrLDs) are intrinsically disordered regions (IDRs) of low sequence complexity with a similar composition to yeast prion domains. PrLDs-containing proteins have been involved in different organisms’ regulatory processes. Regions of moderate amyloid propensity within IDRs have been shown to assemble autonomously into amyloid fibrils. These sequences tend to be rich in polar amino acids and often escape from the detection of classical bioinformatics screenings that look for highly aggregation-prone hydrophobic sequence stretches. We defined them as cryptic amyloidogenic regions (CARs) and recently developed an integrated database that collects thousands of predicted CARs in IDRs. CARs seem to be evolutionary conserved among disordered regions because of their potential to stablish functional contacts with other biomolecules. Here we have focused on identifying and characterizing CARs in prion-like proteins (pCARs) from plants, a lineage that has been poorly studied in comparison with other prionomes. We confirmed the intrinsic amyloid potential for a selected pCAR from Arabidopsis thaliana and explored functional enrichments and compositional bias of pCARs in plant prion-like proteins

Some Learning Objects to Explain Kepler s Laws

In this paper, we present some learning objects for the study of Kepler’s laws that graphically show the orbits and the movements of various planets. One of them shows the orbit of a planet from the point of view of a fixed planet, showing that the orbit is quite involved. No differentials equations are required, but only elementary vector calculus. The learning objects have been implemented in Matlab. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 1–7, 2013; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.20446Benítez López, J.; Gimenez Valentin, MH.; Hueso Pagoaga, JL.; Martínez Molada, E.; Riera Guasp, J. (2013). Some Learning Objects to Explain Kepler s Laws. Computer Applications in Engineering Education. 21:1-7. doi:10.1002/cae.20446S172

ANÁLISIS CONTRASTIVO DE LA ENTONACIÓN DEL ESPAÑOL BOGOTANO Y DEL ESPAÑOL DE SAN JUAN EN FRASES ENTONATIVAS SIMPLES

El presente trabajo tiene como objetivo analizar la entonación de una variedad del español hablado en San Juan (Puerto Rico) y en Bogotá (Colombia) mediante la aplicación del modelo autosegmental y métrico (am) a un corpus constituido por 80 enunciados declarativos simples extraídos de entrevistas realizadas a 20 estudiantes universitarios (hombres y mujeres) nacidos en ambas ciudades capitales. Mediante la aplicación del modelo am, se analizan los movimientos tonales asociados con las sílabas tónicas y con los extremos de las oraciones, con el objetivo de describir la organización fonológica de los enunciados y establecer un repertorio de unidades que conforman los diversos contornos entonativos recurrentes en las dos variedades dialectales estudiadas. La descripción y el análisis de la organización fonológica de los contornos entonativos permitieron postular la existencia de acentos contrastivos tanto a nivel interdialectal como intradialectal

In vitro Natural Killer Cell Immunotherapy for Medulloblastoma

How the immune system attacks medulloblastoma (MB) tumors effectively is unclear, although natural killer (NK) cells play an important role in immune defense against tumor cells. Interactions between receptors on NK cells and ligands expressed by tumor cells are critical for tumor control by immunotherapy. In this study, we analyzed tumor samples from 54 MB patients for expression of major histocompatibility complex class I-related chains A (MICA) and UL16 binding protein (ULPB-2), which are ligands for the NK group 2 member D activatory receptor (NKG2D). The percentage of MICA and ULBP-2 positive cells was higher than 25% in 68% and 6% of MB patients, respectively. A moderate-high intensity of MICA cytoplasmic staining was observed in 46% MB patients and weak ULBP-2 staining was observed in 8% MB patients. No correlation between MICA/ULBP-2 expression and patient outcome was found. We observed that HTB-186, a MB cell line, was moderately resistant to NK cell cytotoxicity in vitro. Blocking MICA/ULBP-2 on HTB-186, and NKG2D receptor on NK cells increased resistance to NK cell lysis in vitro. However, HLA class I blocking on HTB-186 and overnight incubation with IL-15 stimulated NK cells efficiently killed tumor cells in vitro. We conclude that although NKG2D/MICA-ULBP-2 interactions have a role in NK cell cytotoxicity against MB, high expression of HLA class I can protect MB from NK cell cytotoxicity. Even so, our in vitro data indicate that if NK cells are appropriately stimulated, they may have the potential to target MB in vivo

Cell dedifferentiation and epithelial to mesenchymal transitions during intestinal regeneration in H. glaberrima

<p>Abstract</p> <p>Background</p> <p>Determining the type and source of cells involved in regenerative processes has been one of the most important goals of researchers in the field of regeneration biology. We have previously used several cellular markers to characterize the cells involved in the regeneration of the intestine in the sea cucumber <it>Holothuria glaberrima</it>.</p> <p>Results</p> <p>We have now obtained a monoclonal antibody that labels the mesothelium; the outer layer of the gut wall composed of peritoneocytes and myocytes. Using this antibody we studied the role of this tissue layer in the early stages of intestinal regeneration. We have now shown that the mesothelial cells of the mesentery, specifically the muscle component, undergo dedifferentiation from very early on in the regeneration process. Cell proliferation, on the other hand, increases much later, and mainly takes place in the mesothelium or coelomic epithelium of the regenerating intestinal rudiment. Moreover, we have found that the formation of the intestinal rudiment involves a novel regenerative mechanism where epithelial cells ingress into the connective tissue and acquire mesenchymal phenotypes.</p> <p>Conclusions</p> <p>Our results strongly suggest that the dedifferentiating mesothelium provides the initial source of cells for the formation of the intestinal rudiment. At later stages, cell proliferation supplies additional cells necessary for the increase in size of the regenerate. Our data also shows that the mechanism of epithelial to mesenchymal transition provides many of the connective tissue cells found in the regenerating intestine. These results present some new and important information as to the cellular basis of organ regeneration and in particular to the process of regeneration of visceral organs.</p

La simulación digital como apoyo para la visualización de procesos ondulatorios

Una de las líneas que venimos desarrollando en la enseñanza de la Física en la E.U.I.T.I. de la Universidad Politécnica de Valencia, es la de la simulación digital, utilizando animaciones, de procesos físicos relativamente complejos, tales como los ondulatorios. En esta comunicación se muestran mediante el programa ONDAS las posibilidades de este tipo de simulación para explicar conceptos tales como: ondas longitudinales, transversales y mixtas; ondas electromagnéticas; ondas estacionarias; dispersión; absorción; ...One of the guidelines that we are developing in Physics Education in the E.U.I.T.I. in the Universidad Politécnica de Valencia, is digital simulation, by animations, of relatively complex physical processes, such as wave processes. In this presentation, the program ONDAS shows the possibilities of digital simulation for explaining concepts such as: longitudinal, transverse and mixed waves; electromagnetic waves; standing waves; dispersion; absorption; ..

Estudio con EF del método asintótico para la predicción de vida a fatiga bajo fretting en uniones con contacto conforme

La concordancia, bajo determinados supuestos, del campo tensional elástico en las inmediaciones de una entalla aguda (soluciones de Williams) con el existente en el borde de un punzón deslizando sobre un sustrato del mismo material sugiere la posibilidad de emplear las soluciones de Williams para el cálculo de vida a fatiga bajo fretting de uniones a presión. De corroborarse esta afinidad, esta analogía entre el comportamiento de piezas entalladas y contactos conformes permitiría realizar ensayos con probetas entalladas en lugar de los complicados ensayos de fretting. El objetivo del presente artículo es analizar mediante elementos finitos la validez de dicha analogía, dejando una herramienta preparada para el estudio de la influencia que puedan tener otros factores entre los que se encuentra la plastificación de la zona gobernada por la singularidad.The resemblance between the elastic stress field in a sharp V-notch and a sliding punch on a substrate of the same material, provided some requirements are fulfilled, suggests the possibility of using the former in fretting-fatigue life predictions of complete locally slipping contact problems. In case that this analogy between both stress fields be corroborated, it will allow to test sharp V-notch specimens instead of doing complex fretting-fatigue tests, much more difficult to handle with. In this paper, a finite element analysis is carried out in order to evaluate the validity of such an analogy, getting a ready tool for studying the influence that further factors could have on it, as is the case of cyclic yielding near the punch corners

Acute effects of a single tennis match on passive shoulder rotation range of motion, isometric strength and serve speed in professional tennis players

Shoulder pain has been associated with glenohumeral internal rotation deficit (GIRD) and a reduction in external rotation (ER) strength; however, in tennis players, there is scarce evi- dence regarding the impact of a single match on shoulder range of motion (ROM), strength and serve speed. The aim of this study was to determine the acute effect of a single tennis match on shoulder rotation ROM, isometric strength and serve speed. Twenty-six profes- sional tennis players participated in the study (20.4±4.4 years; 10.5±3.2 years tennis exper- tise; 20.5±5.4 h/week training). Passive shoulder external (ER-ROM) and internal rotation ROM (IR-ROM), ER and IR isometric strength were measured before and after a single ten- nis match (80.3±21.3 min) in both shoulder´s. Moreover, the total arc of motion (TAM) and ER/IR strength ratio were calculated. Video analysis was used to assess the number of serves and groundstrokes, while a radar gun was utilized to measure maximal ball speed. In the dominant shoulder, compared to pre-match levels, IR-ROM was significantly reduced (-1.3%; p = 0.042), while ER-ROM (5.3%; p = 0.037) and TAM (3.1%; p = 0.050) were signif- icantly increased. In the non-dominant shoulder, ER-ROM (3.7%; p = 0.006) was increased. Furthermore, in the dominant shoulder, the isometric ER strength was significantly reduced after the match (-4.8%; p = 0.012), whereas serve speed was not significantly reduced after match (-1.16%; p = 0.197). A single tennis match leads to significant reductions in shoulder ROM (e.g., IR of the dominant shoulder) and isometric strength (e.g., ER of the dominant shoulder). This study reveals the importance of recovery strategies prescription aiming at minimize post-match alteration in the shoulders.SIThe authors would like to express their gratitude to the tennis players for their participation.The authors received no specific funding for this work