Alkaloids from leaves of Guatteria pogonopus (Annonaceae) and their Cytotoxicities

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-02-04T12:04:40Z No. of bitstreams: 1 SANTOS, M.F. Alkaloids from leaves... 2018.pdf: 730144 bytes, checksum: d7e0d9cedb7ba9d4c04d12104109764f (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-02-04T12:20:05Z (GMT) No. of bitstreams: 1 SANTOS, M.F. Alkaloids from leaves... 2018.pdf: 730144 bytes, checksum: d7e0d9cedb7ba9d4c04d12104109764f (MD5)Made available in DSpace on 2019-02-04T12:20:05Z (GMT). No. of bitstreams: 1 SANTOS, M.F. Alkaloids from leaves... 2018.pdf: 730144 bytes, checksum: d7e0d9cedb7ba9d4c04d12104109764f (MD5) Previous issue date: 2018CNPq, CAPES, FINEP, FAPEAM, Fundação Araucária, FAPITEC/SE, UFAM, UFS, and UFPR for financial support and fellowship.Universidade Federal de Sergipe. Departamento de Química. São Cristóvão, SE, Brasil / Universidade Federal do Paraná. Centro de Ressonância Magnética Nuclear. Curitiba, PR, Brasil.Universidade Federal de Sergipe. Departamento de Química. São Cristóvão, SE, Brasil.Universidade Federal do Paraná. Centro de Ressonância Magnética Nuclear. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Sergipe. Departamento de Química. São Cristóvão, SE, Brasil.Universidade Federal do Paraná. Centro de Ressonância Magnética Nuclear. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil.Universidade Federal do Vale do São Francisco. Núcleo de Estudos e Pesquisas de Plantas Medicinais. Petrolina, PE, Brasil.Universidade do Estado do Amazonas. Escola Superior de Ciências da Saúde. Manaus, AM, Brasil.Universidade Federal do Paraná. Centro de Ressonância Magnética Nuclear. Curitiba, PR, Brasil.Universidade Federal do Amazonas. Departamento de Química. Manaus, AM, Brasil.The phytochemical investigation of the alkaloid-rich fraction obtained from the leaves of Guatteria pogonopus Mart. (Annonaceae) allowed the isolation and identification for the first time in this species of: (+)-nornuciferine (1), a mixture of 1 and (+)-anonaine (2), (+)-isocorydine (3), (+)-nuciferine (4), (+)-roemerine (5), (−)-tetrahydropseudocolumbamine (6), a mixture of 6, liriodenine (9) and lysicamine (10), a mixture of 1,2,9-trimethoxy-10-hydroxyaporphine (7) and bulbocapnine (8), 9, 10, and (+)-N-methyllindicarpine (11). Compounds 6, 7, 8, and 11 have not been previously reported in the family Annonaceae. Furthermore, the formerly synthetic 1,2,9-trimethoxyaporfin-10-ol (7) is described for the first time as a natural aporphine alkaloid herein. The chemical structures were established by 1D and 2D NMR as well as in comparison with data previously reported in the literature. The cytotoxic activity of the alkaloids was evaluated against tumor (B16-F10, HepG2, HL-60, and K562) and non-tumor (PBMC) cell lines. Alkaloid 1 presented significant activity against HepG2 cell lines with IC50 of 9.60 μmol L-1 while the mixture of 6, 9 and 10 displayed strong cytotoxic activity against HL-60 and K562 cell lines with IC50 values of 3.41 an 8.50 μmol L-1, respectively

ALKALOIDS FROM LEAVES OF GUATTERIA POGONOPUS (ANNONACEAE) AND THEIR CYTOTOXICITIES

The phytochemical investigation of the alkaloid-rich fraction obtained from the leaves of Guatteria pogonopus Mart. (Annonaceae) allowed the isolation and identification for the first time in this species of: (+)-nornuciferine (1), a mixture of 1 and (+)-anonaine (2), (+)-isocorydine (3), (+)-nuciferine (4), (+)-roemerine (5), (-)-tetrahydropseudocolumbamine (6), a mixture of 6, liriodenine (9) and lysicamine (10), a mixture of 1,2,9-trimethoxy-10-hydroxyaporphine (7) and bulbocapnine (8), 9, 10, and (+)-N-methyllindicarpine (11). Compounds 6, 7, 8, and 11 have not been previously reported in the family Annonaceae. Furthermore, the formerly synthetic 1,2,9-trimethoxyaporfin-10-ol (7) is described for the first time as a natural aporphine alkaloid herein. The chemical structures were established by 1D and 2D NMR as well as in comparison with data previously reported in the literature. The cytotoxic activity of the alkaloids was evaluated against tumor (B16-F10, HepG2, HL-60, and K562) and non-tumor (PBMC) cell lines. Alkaloid 1 presented significant activity against HepG2 cell lines with IC50 of 9.60 µmol L-1 while the mixture of 6, 9 and 10 displayed strong cytotoxic activity against HL-60 and K562 cell lines with IC50 values of 3.41 an 8.50 µmol L-1, respectively

Hydroalcoholic Leaf Extract of <i>Punica granatum</i>, alone and in Combination with Calcium Hydroxide, Is Effective against Mono- and Polymicrobial Biofilms of <i>Enterococcus faecalis</i> and <i>Candida albicans</i>

Failures in endodontic treatments are mostly associated with the difficulty in eradicating microbes of the root canal system, highlighting the need to develop novel effective antimicrobials. Punica granatum (pomegranate) leaf hydroalcoholic extract may be a potential alternative in canal dressing, owing to its antimicrobial properties. The objective of this study was to evaluate the antimicrobial activity of hydroalcoholic leaf extract of Punica granatum (HEPg) alone or in combination with calcium hydroxide (Ca(OH)2) against Enterococcus faecalis and Candida albicans in isolation and in mono- and polymicrobial biofilms. Microdilution tests in broth and assays for inhibition of biofilm formation were carried out to evaluate the antimicrobial properties of HEPg and HEPg + Ca(OH)2 against Enterococcus faecalis and Candida albicans. The cytotoxicity of HEPg in HaCaT cells was evaluated by MTT assay. HEPg and HEPg + Ca(OH)2 exerted significant antimicrobial activity against planktonic cells and mono- and polymicrobial biofilms. The combination of Punica granatum extract with Ca(OH)2 appears to be a promising alternative in endodontic treatments, which could be tested in vivo to confirm the efficacy of this mixture in disinfecting root canal systems

Resumos em andamento - Educação

Resumos em andamento - Educaçã

Resumos em andamento - Educação

Resumos em andamento - Educaçã