ROM THE HISTORY OF TRADE AND BANKING IN SVISHTOV. TRADING-AND-BANKING (MONEY-CHANGING) COMPANIES AND BANKING HOUSES AND THEIR IMPORTANCE FOR THE TOWN ECONOMY (1878-1912)

Based on archival sources not used so far, periodicals and other sources of information, the study reviews the economic life of Svishtov in the period 1878-1912, with an emphasis on the development of trade. Against this background, it examines the issue of re-directing trading capital to banking. The focus of the study is the trading-and-banking (money-changing) companies and banking houses (8 partnerships and 9 sole proprietorships). The study answers a wide range of questions concerning their emergence, institutional characteristics, the nature of the capital mobilized in them; the banking operations and services they offered, with a focus on credit and the circumstances that allowed banking houses at certain periods to make a greater contribution to lending money to local trade and production; the psychological profile of the Svishtov merchant; the reasons why the joint-stock principle did not establish itself firmly and permanently in the economic life of the town. It also reveals the leading role of the Bulgarian National Bank in lending money to local trade and to the two savings joint-stock companies that existed in the 1890s and shows the importance of the banking houses for the development of the town’s economy in three directions – they were subjects in the process of initial capital accumulation; they provided short-term credit and increased the common funds for performing payment operations; they brought stability to the functioning of the banking and lending system especially in times of changes in the central bank’s policy. They also played the role of a ‘financial buffer’ that ensured the survival of companies in times of temporary hardships. The conclusion reached is that being away from joint-stock entrepreneurship, especially in banking, and the inability of local economic forces to create a jointstock bank, was one of the main reasons for the economic backwardness of the town, beginning at the end of the nineteenth century

Diet composition of the lizard Lacerta viridis (Laurenti, 1768) (Reptilia: Lacertidae) in Bulgaria confirm its generalistic feeding behaviour

The eastern green lizard (Lacerta viridis) is a mostly insectivorous species, based on multiple studies from across its range. However, for Bulgaria the published data of such kind are limited to five publications. We investigated faecal samples from a total of 60 individuals of free-ranging L. viridis obtained from two localities in western Bulgaria. Our aim was to clarify the diet regarding its taxonomic composition, as well as some physical characteristics of the prey like hardness and evasiveness. For one of the study sites we compared the realised trophic niche (prey items from the faecal pellets) with the fundamental niche (invertebrates collected via pit-fall traps exposure). In our results, two invertebrate taxa had the largest share (both in frequency and abundance) in the food spectrum of L. viridis: Araneae and Coleoptera), respectively 21.2% and 17.6%. Regarding specific prey selectivity, analysis showed that Lepidoptera are most preferred (E* = 0.68), and Formicidae - most avoided prey items (E* = -0.79). We registered several cases of saurophagy (in four samples) and keratophagy (in two samples), two types of dietary items, which have not been reported for L. viridis so far

BULGARIAN LAND AND ITS NATURAL RESOURCES – LATE 12TH CENTURY – 14TH CENTURY

В настоящата статия се разкрива представата за българската държава и нейните природни дадености в западноевропейските извори за периода кр. на XII-XIV в. За постигане на тази цел се анализират и систематизират публикуваните у нас извори от западен произход – хроники, истории, пътеписи, космографски описания, доклади, географски карти, портолани и др. В тях са отразени впечатленията и познанията на западния летописец за границите на българската държава и нейните природни дадености. Те разкриват, че българите и тяхната земя намират достойно място в трудовете на западноевропейските автори. The author of this article has made an attempt to reveal how the Bulgarian state and its natural resources have been described in West European sources of information during the period from the late 12th century to the 14th century. In order to achieve the aim of the article the West European sources of information published in Bulgaria have been analyzed and systematized, incl. chronicles, stories, travelogues, cosmographic maps, reports, maps, portolans, etc. They reveal the impressions and knowledge of western annalists and chroniclers on the borders of the Bulgarian state and its natural resources. They also show that Bulgarians and their land occupy a worthy place in the works of western authors

Winter activity of the snake-eyed lizard Ophisops elegans (Reptilia: Lacertidae) in the northwesternmost part of its range

There is a lack of information for potential winter activity of the snake-eyed lizard Ophisops elegans in Europe, where it has a limited distribution. To test the hypothesis that this species can be active during the winter months, two locations in Bulgaria were chosen. The visits were conducted in January, February and December 2022 near the village of Meden Buk and in December 2022 above the village of Mezek. A total of 19 individuals of O. elegans were recorded. In addition, we confirmed foraging behaviour based on faecal sample collection. To our knowledge, this is the northernmost record of winter activity in the snake-eyed lizard and the first consecutive observation of year-round activity for this species in Europe

An extended mtDNA phylogeography for the alpine newt illuminates the provenance of introduced populations

Many herpetofauna species have been introduced outside of their native range. MtDNA barcoding is regularly used to determine the provenance of such populations. The alpine newt has been introduced across the Netherlands, the United Kingdom and Ireland. However, geographical mtDNA structure across the natural range of the alpine newt is still incompletely understood and certain regions are severely undersampled. We collect mtDNA sequence data of over seven hundred individuals, from both the native and the introduced range. The main new insights from our extended mtDNA phylogeography are that 1) haplotypes from Spain do not form a reciprocally monophyletic clade, but are nested inside the mtDNA clade that covers western and eastern Europe; and 2) haplotypes from the northwest Balkans form a monophyletic clade together with those from the Southern Carpathians and Apuseni Mountains. We also home in on the regions where the distinct mtDNA clades meet in nature. We show that four out of the seven distinct mtDNA clades that comprise the alpine newt are implicated in the introductions in the Netherlands, United Kingdom and Ireland. In several introduced localities, two distinct mtDNA clades co-occur. As these mtDNA clades presumably represent cryptic species, we urge that the extent of genetic admixture between them is assessed from genome-wide nuclear DNA markers. We mobilized a large number of citizen scientists in this project to support the collection of DNA samples by skin swabbing and underscore the effectiveness of this sampling technique for mtDNA barcoding

Diet of the Viviparous lizard Zootoca vivipara (Lichtenstein, 1823) (Reptilia: Lacertidae) from its southern range

The Viviparous lizard Zootoca vivipara (Lichtenstein, 1823) is widespread across Eurasia, but in Bulgaria it is considered a glacial relict, restricted to the high mountains. In spite of its wide distribution, there is still lack of knowledge on its dietary habits, especially from the southern part of the range. In order to shed light on its diet, we conducted а study in three mountain sites in Bulgaria (Vitosha, Rila and Stara Planina Mtns.). They were visited in 2016 and 2017 during the lizard's active season (May–September); 343 Viviparous lizards were captured by hand and measured and weighed. To investigate the food preferences, we adopted a non-invasive method, obtaining faecal samples, which were then analysed under a stereoscopic microscope. A total of 290 faecal samples that could be assigned to individual specimens with determined age and sex were collected. We calculated the relative abundance and occurrence of each prey category. Data were compared by sex and age classes (immatures vs. adults) as a whole and for each of the three sites. A total of 19 prey categories were detected and the number of categories per study site varied between 16 and 18. The most common invertebrates were Araneae and Auchenorrhyncha. Statistically significant differences between immatures and adults were found as adults showed more diverse diet. No such differences between sexes were detected

Unravelling the origin of the common wall lizards (Podarcis muralis) in south-eastern Europe using mitochondrial evidence

The origin of the common wall lizards (Podarcis muralis) populations in south-eastern Europe (namely in Bulgaria and Romania), representing the north-eastern range border of this species, was addressed using mitochondrial DNA. We compared cytochrome b sequences from Bulgaria and Romania with those from the contiguous range in Central Europe that are available from previous studies. We recorded five main haplogroups in Bulgaria and Romania, belonging to the Central Balkan clade. However, haplogroup III was recorded in more localities than previously found. Additionally, signs of haplotype admixture were identified in several populations along the Danube River. The presence of the Southern Alps haplotype in one population from Otopeni, Bucharest (Romania) and its close phylogenetic relationships to north Italy populations suggests human-mediated introductions of this wall lizard clade in Romania. Our results confirm that P. muralis can have non-native lineages and admixture through active human-mediated transport

Analyses of Skin Secretions of Vipera ammodytes (Linnaeus, 1758) (Reptilia: Serpentes), with Focus on the Complex Compounds and Their Possible Role in the Chemical Communication

Snakes rely heavily on chemical cues when foraging, searching for mates, etc. Snakes’ sex attractiveness pheromones comprise mainly heavy, semi-volatile compounds such as ketones. Here we investigated the composition of skin secretions of adult Vipera ammodytes (Linnaeus, 1758) individuals. The samples were analyzed by gas chromatography/mass spectrometry and the identification of the compounds was performed using commercial mass spectral libraries and retention times. The relative concentrations of all detected compounds were tested for significant differences between (1) male vs. female live individuals, (2) shed skin vs. live individuals, and (3) pre-reproductive vs. reproductive live individuals. We detected fifty-nine compounds of which six were ketones. Two ketones (2-pentacosanone and 2-heptacosanone) were present in many of the samples and thus may have an important role in the V. ammodytes chemical communication. We did not find significant differences between the relative concentrations of the compounds between male and female individuals (only three compounds are exceptions). Significant differences were found between extracts from shed skins and live individuals and between live pre-reproductive individuals and live reproductive individuals. The results of the study suggest that chemical communication in V. ammodytes involves less compounds in comparison to the known literature data for other species

Pathogenic Potential of Opportunistic Gram-Negative Bacteria Isolated from the Cloacal Microbiota of Free-Living Reptile Hosts Originating from Bulgaria

Reptiles are known to be asymptomatic carriers of various zoonotic pathogens. A number of Gram-negative opportunistic commensals are causative agents of bacterial infections in immunocompromised or stressed hosts and are disseminated by reptiles, whose epidemiological role should not be neglected. Since most studies have focused on exotic species, in captivity or as pet animals, the role of wild populations as a potential source of pathogens still remains understudied. In the present study, we isolated a variety of Gram-negative bacteria from the cloacal microbiota of free-living lizard and tortoise hosts (Reptilia: Sauria and Testudines) from the Bulgarian herpetofauna. We evaluated their pathogenic potential according to their antibiotic susceptibility patterns, biofilm-forming capacity, and extracellular production of some enzymes considered to play roles as virulence factors. To our knowledge, the phenotypic manifestation of virulence factors/enzymatic activity and biofilm formation in wild reptile microbiota has not yet been widely investigated. All isolates were found to be capable of forming biofilms to some extent and 29.6% of them could be categorized as strong producers. Two strains proved to be excellent producers. The majority of the isolated strains showed extracellular production of at least one exoenzyme. The most pronounced pathogenicity could be attributed to the newly isolated Pseudomonas aeruginosa strain due to its multiresistance, excellent biofilm formation, and expression of exoenzymes

Comparison of methodologies for the extraction of snakes’ (Reptilia: Serpentes) skin secretions and preliminary results on the presence of pheromones

During this preliminary study, 26 specimens from 8 different snake species were examined. Three methodologies for extraction of skin secretions by immersion of skin in n-hexane were used: immersing the whole body of live individuals for approximately 1 min in the field (n = 13), immersing the whole body of dead specimens (found freshly killed on road) for 24 h (n = 4), and soaking a shed skin for 24 h (n = 9). We did not immerse the head and tail to avoid contamination of the samples. All samples were collected during snakes’ active period (February – first half of June) and were analyzed by gas chromatography-mass spectrometry (GC/qMS). Based on the prevalence in the fraction of long-chained hydrocarbons, lipids or lipids with ketones we can separate the samples in three categories. The lipid fractions were present mainly in the shed skin samples, while the long-chained hydrocarbons dominated in the live specimens. According to the literature, ketones are the main component of the snakes’ pheromone communication. We found ketones in five samples, three live and two dead specimens, from five different species (Vipera ammodytes (Linnaeus, 1758), V. berus (Linnaeus, 1758), Dolichophis caspius (Gmelin, 1789), Natrix natrix (Linnaeus, 1758), and Platyceps najadum (Eichwald, 1831)). No ketones were found in the shed skin extracts. Ketones were found only in adults, both males and females. Presence of ketones was observed only for the period from the end of May to the beginning of June. Based on the preliminary results, we can suggest that secretion extraction from live specimens in the field could prove a valuable method in studies on snake pheromones in addition to the already developed methodologies. We speculate that the pheromones are present only in the reproductive and probably post-reproductive period. However, further research with larger sample size is required