Dobivanje nadomjestka mliječne masti iz majčina mlijeka interesterifikacijom biljnih ulja

Palm oil, palm kernel oil, olive oil, sunflower oil, and marine oil blend, formulated in the mass ratio of 4.0:3.5:1.0:1.5:0.2, was subjected to interesterification catalyzed by lipase from Thermomyces lanuginosa (Lipozyme® TL IM) for obtaining a product that contains similar triacylglycerol (TAG) structure to that of human milk fat (HMF). Reactions were carried out in a double jacketed glass vessel equipped with magnetic stirrer at 60 °C for 2, 4, 6, 8, 12 and 24 h. The blend was analyzed for fatty acid composition of both total fatty acids and those at the sn-2 position after pancreatic lipase hydrolysis. After interesterification, TAGs were purified by thin layer chromatography and TAG species were determined according to the carbon number (CN) by high-temperature gas chromatography. Enzymatic interesterification generated significant differences for all TAG species from CN30 to CN54. Concentrations of some TAG species (CN30, 32, 34, 36, 38, 50, 52 and 54) decreased, while some (CN40 to 48) increased after 24 h. TAG species with higher CN reached maximum levels at the end of 6 h of reaction time. The predominant TAGs of the reaction product after 24 h were CN46, 48, 50, 52 and 54 with ratios of 13.8, 18.2, 13.9, 17.8, and 12.1 %, respectively. These TAG species contain mainly 1,3-diunsaturated-2-saturated structure, like HMF.Interesterifikacijom mješavine palmina ulja, ulja koštice palme, maslinova, suncokretova i ribljeg ulja (maseni omjer 4,0:3,5:1,0:1,5:0,2), ubrzane lipazom iz Thermomyces lanuginosa (Lipozyme® TL IM), dobiven je proizvod po sastavu sličan triacilglicerolu iz majčina mlijeka. Postupak je proveden u posudama s dvostrukom stijenkom i magnetskom miješalicom na 60 °C tijekom 6, 8, 12 i 24 h. Istražen je sastav ukupnih masnih kiselina i onih u sn-2 položaju nakon hidrolize pomoću lipaze gušterače. Nakon interesterifikacije triacilgliceroli su izdvojeni tankoslojnom kromatografijom, a broj ugljikovih atoma utvrđen je plinskom kromatografijom pri visokoj temperaturi. Interesterifikacija je uzrokovala velike razlike u sastavu svih triacilglicerola s 30-54 ugljikovih atoma. Smanjile su se koncentracije nekih triacilglicerola (sa 30, 32, 34, 36, 38, 50, 52 i 54 ugljikova atoma), dok su se neke (onih sa 40 do 48 ugljikovih atoma) povećale nakon 24 h. Nakon 6 sati reakcije bilo je najviše triacilglicerola s većim brojem ugljikovih atoma. Nakon 24 sata prevladali su triacilgliceroli sa 46, 48, 50, 52 i 54 ugljikova atoma. Njihov je udio iznosio: 13,8; 18,2; 13,9; 17,8 i 12,1 %. Struktura tih triacilglicerola bila je slična onima iz majčina mlijeka

VIRTUAL LEARNING IS NOT FOR MY CHILD! A PARENTAL PERSPECTIVE OF PRACTICES USED WITH CHILDREN WITH AUTISM DURING THE PANDEMIC

Once the COVID-19 was declared as a pandemic, most countries temporarily closed schools and shifted to home-based distance education. Each country had its own way of implementing education remotely. Turkey used an enhanced version of the currently existing Education Information Network (EBA) to deliver distance education to all children including those with autism. The rapid shift from face-to-face education to home-based virtual learning created unprecedented challenges and impacted development and learning of children with autism who often need individualized and systematic instruction. Therefore, the purpose of this study was to explore parental perceptions about effectiveness of distance education practices for children with autism and challenges they faced during visual learning. A total of 208 parents of children with autism participated in quantitative data collection while 18 also attended to individually conducted interviews. Results indicated many families did not use EBA to support their children’s learning and the content of virtual learning opportunities through EBA was not appropriate for the characteristics of children with autism. Implications for future practice and research as well as the limitations of this study were discussed

Determination of Primary Surface Water Pollution Indicators by Multivariate Statistical Techniques in an Industrialized Basin

This study aimed to investigate the point and diffuse pollution sources of high total phosphorus (TP) pollution detected in the dry and wet seasons of the industrialized and urbanized Saz-Cayirova Basin through field observation and multivariate statistical techniques. In this context, nineteen water quality parameters were analyzed in surface water samples collected monthly between June 2020 and July 2021 from nineteen different sites along the Saz-Cayirova stream. Firstly, two reference sites representing a better surface water status were determined and assessed the water quality on the stream tributaries affecting the wastewater treatment plant in the two Organized Industrial Zones (OIZ). Secondly, hierarchical cluster analysis (HCA) and principal component analysis (PCA) were performed to evaluate the complex water quality dataset and reveal the latent sources of TP pollution. The results showed that the tributaries in the pressure of OIZ discharges were highly enriched in COD, TOC, NO3-N, NH3-N, and TP concentrations compared to the reference level. Compared with reference sites, the concentrations of the four heavy metals examined were at a plausible level. Besides, the pollution sources of TP were industrial processes wastewater such as dyeing, washing, phosphating, domestic wastewater from OIZ, urban diffuse waters with organic character, and continuous discharges of undefined sources. The seasonal variation of TP values is observed relatively low, indicating that the stream network is greatly affected by point source pollution. Our observation and analysis imply that the treatment technologies adopted by the OIZ wastewaters plant is sufficient to treat heavy metals. However, measures need to be formulated to remove TP and organic pollution from the secondary production process. © 2022, Avestia Publishing. All rights reserved

FGF-23 and vascular dysfunction in patients with stage 3 and 4 chronic kidney disease

Studies in animals show that fibroblast growth factor (FGF)-23 interferes with vascular reactivity induced by the nitric oxide (NO) system. To investigate the relationship between circulating FGF-23 levels and the response of forearm blood flow to ischemia (flow-mediated vasodilatation, FMD) and nitroglycerin, we tested 183 patients with stage 3-4 chronic kidney disease (CKD). None of them had cardiovascular complications or were taking drugs interfering with vascular function. Patients with FGF-23 levels above the median had significantly lower glomerular filtration rate, FMD, and fetuin-A levels (an anti-inflammatory molecule and potent inhibitor of calcification). They also had higher proteinuria and phosphate levels when compared to patients whose FGF-23 levels were below the median. The response to nitroglycerin was not different between the two groups. Multiple regression analysis showed that the relationship between FGF-23 and FMD was only modestly sensitive to adjustment for classical risk factors, biomarkers of bone mineral metabolism, high-sensitivity C-reactive protein, and homeostatic model assessment index. Adjustment for asymmetrical dimethyl arginine (ADMA) weakened the strength of this link; however, it remained highly significant. There was no independent association between FGF-23 and nitroglycerin. Thus, attenuation of FMD by ADMA suggests that this endogenous inhibitor of NO synthase may, in part, mediate the vascular effects of FGF-23 in patients with CKD. © 2010 International Society of Nephrology