Endophytic hyphal compartmentalization is required for successful mycobiont-wheat interaction as revealed by confocal laser microscopy

Non-Peer ReviewedRoot endophytic fungi are seen as promising alternatives to replace chemical fertilizers and pesticides in sustainable and organic agriculture systems. Fungal endophytes structure formations play key roles in symbiotic intracellular association with plant-roots. To compare the morphologies of Ascomycete endophytic fungi in wheat, we analyzed growth morphologies during endophytic development of hyphae within the cortex of living vs dead root cells. Confocal laser scanning microscopy (CLSM) was used to characterize fungal cell morphology within lactofuchsin-stained roots. Cell form regularity Ireg and cell growth direction Idir, indexes were used to quantify changes in fungal morphology. Endophyte fungi in living roots had a variable Ireg and Idir values, low colonization abundance and patchy colonization patterns, whereas the same endophyte species in dead (γ-irradiated) roots had consistent form of cells and mostly grew parallel to the root axis. Knot, coil and vesicle structures dominated in living roots, as putative symbiotic functional organs. Finally, an increased hypha septation in living roots might indicate local specialization within endophytic Ascomycota. Our results suggested that the applied method could be expanded to other septate fungal symbionts

Bulk viscous effects on flow and dilepton radiation in a hybrid approach

Starting from IP-Glasma initial conditions, we investigate the effects of bulk pressure on low mass dilepton production at the Relativistic Heavy Ion Collider (RHIC) and the Large Hadron Collider (LHC) energies. Though thermal dilepton $v_2$ is affected by the presence of both bulk and shear viscosity, whether or not these effects can be measured depends on the dilepton "cocktail" contribution to the the low mass dilepton $v_2$. Combining the thermal and "cocktail" dileptons, the effects of bulk viscosity on total dilepton $v_2$ is investigated.Comment: Proceedings for the 26th International Conference on Ultrarelativistic Nucleus-Nucleus Collisions (Quark Matter 2017), February 5-11 2017, Chicago, Illinois, US