225 research outputs found
Classical sheep scrapie in Great Britain: spatial analysis and identification of environmental and farm-related risk factors
Evidence of scrapie transmission via milk
<p>Abstract</p> <p>Background</p> <p>The risk of scrapie infection increases with increased duration and proximity of contact between sheep at lambing. Scrapie infectivity has not been detected in milk but cellular prion protein, the precursor of disease-associated prion protein PrP<sup>d</sup>, has been found in milk from ruminants. To determine whether milk is able to transmit scrapie, 18 lambs with a prion protein genotype associated with high susceptibility to scrapie (VRQ/VRQ) were fed milk from twelve scrapie-affected ewes of the same genotype, and 15 VRQ/VRQ sheep reared on scrapie-free dams served as controls.</p> <p>Results</p> <p>Three lambs fed milk from scrapie-affected ewes were culled due to intercurrent diseases at 43, 44 and 105 days of age respectively, and PrP<sup>d </sup>was detected in the distal ileum of the first two lambs, whilst PrP<sup>d </sup>was not found in lymphoreticular tissues in the third lamb. A control lamb, housed in a separate pen and culled at 38 days of age, was also negative for PrP<sup>d </sup>in a range of tissues. Samples of recto-anal mucosa associated lymphoid tissue collected from the remaining 15 live lambs at seven months of age (between five to seven months after mixing) were positive for PrP<sup>d </sup>in the scrapie milk recipients, whereas PrP<sup>d </sup>was not detected in the remaining 14 controls at that time. A subsequent sample collected from control lambs revealed PrP<sup>d </sup>accumulation in two of five lambs eight months after mixing with scrapie milk recipients suggestive of an early stage of infection via lateral transmission. By contrast, the control sheep housed in the same building but not mixed with the scrapie milk recipients were still negative for PrP<sup>d</sup>.</p> <p>Conclusion</p> <p>The presence of PrP<sup>d </sup>in distal ileum and rectal mucosa indicates transmission of scrapie from ewe to lamb via milk (or colostrum) although it is not yet clear if such cases would go on to develop clinical disease. The high level of infection in scrapie-milk recipients revealed by rectal mucosal testing at approximately seven months of age may be enhanced or supplemented by intra-recipient infection as these lambs were mixed together after feeding with milk from scrapie-affected ewes and we also observed lateral transmission from these animals to lambs weaned from scrapie-free ewes.</p
Optimization of commodity portfolio management
The problem we consider is introduced by Uljarice Bačka, LLC. The core business activities of the company are trade of agriculture commodities, warehousing and distribution and crops production. The main traded goods are: corn, wheat, barely, sunflower, soybean, soybean meal and raw material for crops production: fertilizers, plant protection products, seeds and other. Since a large part of company’s activities relays on corn, predicting the price of that good is of the main interest. In order to make a reasonable predictions, models which incorporate the crucial factors influencing the corn prices are needed. Of course, the important issue is which data are available. Within the data that we obtained, a correlation analysis is performed to point out the relevant parameters. We introduce different methods for obtaining the predictions and provide some numerical results
Relevant but neglected habitat types by the Directive 92/43 EEC in southern Italy
The 92/43/EEC Habitats Directive is the main European Union legal tool concerning nature conservation. The habitat types listed in Annex I to the Directive are phytosociology-based. It is widely acknowledged that phytosociological analysis is a crucial approach for habitats characterization and for monitoring their conservation status. Based on bibliographic investigations and new field survey campaigns, a list of habitat types neglected by the Habitats Directive is here presented and described for southern Italy. In this paper, 8 new habitat types and 13 subtypes are proposed. For each of these proposed new habitat types, a wide range of information, including ecology, chorology, species composition, syntaxonomy, threats, and conservation status, is here provided. To supply more detailed phytogeographical and coenological information about the proposed new habitat types, distribution maps based on 10 x 10 km reference grids and phytosociological tables including unpublished releves were carried out
Detection and Antimicrobial Resistance Profile of Enteropathogenic (EPEC) and Shigatoxigenic Escherichia coli (STEC) in Conventional and Organic Broiler Chickens
ABSTRACT Enteropatogenic Escherichia coli (EPEC) and shigatoxigenic E. coli (STEC), are generally poultry and poultry product isolate and can cause serious human infections. Many strains may become resistant to various antimicrobials, which can hinder the treatment of bacterial diseases. Organic farming seeks to avoid the selection and frequency of antimicrobial-resistant bacteria. This study aims to verify the resistance of EPEC and STEC from organic and conventional (industrial) broiler isolates to antimicrobials. All isolates were submitted to disk diffusion test with tetracycline, gentamicin, enrofloxacin, ceftriaxone and amoxicillin + clavulanate (TET, GEN, ENO, CTX, AMC) and PCR to detect specific virulence genes for EPEC and STEC. A total of 297 E. coli strains were isolated, 213 from conventional. In organic broiler, 84 strains were isolated. The strains from the conventional broiler isolates were resistant to five antimicrobials tested: TET 48.82% (104/213), ENO 28.17% (60/213), CTX 15.49% (33/213), GEN 14.55% (31/213), and AMC 7.04% (15/213), and 9.86% (21/213) were considered multidrug-resistant. Organic chicken strains were resistant to four of the antimicrobials tested: TET 35.7% (30/84), ENO 9.5% (8/84), CTX 2.4% (2/84), GEN 4.8% (4/84). Of the strains from the organic broiler chicken isolates, only 1.2% (1/84) was considered multidrug-resistant. No EPEC and STEC were found in the organic chicken samples. The multidrug resistance was characterized in 9.52% (2/21) of the EPEC and 4.76% (1/21) of the STEC. The study demonstrated the absence of EPEC and STEC strains in organic broilers and carcasses and a lower frequency of multiresistant strains compared to conventional breeding
Relationship between clinical signs and postmortem test status in cattle experimentally infected with the bovine spongiform encephalopathy agent
<p>Abstract</p> <p>Background</p> <p>Various clinical protocols have been developed to aid in the clinical diagnosis of classical bovine spongiform encephalopathy (BSE), which is confirmed by postmortem examinations based on vacuolation and accumulation of disease-associated prion protein (PrP<sup>d</sup>) in the brain. The present study investigated the occurrence and progression of sixty selected clinical signs and behaviour combinations in 513 experimentally exposed cattle subsequently categorised postmortem as confirmed or unconfirmed BSE cases. Appropriate undosed or saline inoculated controls were examined similarly and the data analysed to explore the possible occurrence of BSE-specific clinical expression in animals unconfirmed by postmortem examinations.</p> <p>Results</p> <p>Based on the display of selected behavioural, sensory and locomotor changes, 20 (67%) orally dosed and 17 (77%) intracerebrally inoculated pathologically confirmed BSE cases and 21 (13%) orally dosed and 18 (6%) intracerebrally inoculated but unconfirmed cases were considered clinical BSE suspects. None of 103 controls showed significant signs and were all negative on diagnostic postmortem examinations. Signs indicative of BSE suspects, particularly over-reactivity and ataxia, were more frequently displayed in confirmed cases with vacuolar changes in the brain. The display of several BSE-associated signs over time, including repeated startle responses and nervousness, was significantly more frequent in confirmed BSE cases compared to controls, but these two signs were also significantly more frequent in orally dosed cattle unconfirmed by postmortem examinations.</p> <p>Conclusions</p> <p>The findings confirm that in experimentally infected cattle clinical abnormalities indicative of BSE are accompanied by vacuolar changes and PrP<sup>d </sup>accumulation in the brainstem. The presence of more frequently expressed signs in cases with vacuolar changes is consistent with this pathology representing a more advanced stage of disease. That BSE-like signs or sign combinations occur in inoculated animals that were not confirmed as BSE cases by postmortem examinations requires further study to investigate the potential causal relationship with prion disease.</p
Equilibrium configurations of two charged masses in General Relativity
An asymptotically flat static solution of Einstein-Maxwell equations which
describes the field of two non-extreme Reissner - Nordstr\"om sources in
equilibrium is presented. It is expressed in terms of physical parameters of
the sources (their masses, charges and separating distance). Very simple
analytical forms were found for the solution as well as for the equilibrium
condition which guarantees the absence of any struts on the symmetry axis. This
condition shows that the equilibrium is not possible for two black holes or for
two naked singularities. However, in the case when one of the sources is a
black hole and another one is a naked singularity, the equilibrium is possible
at some distance separating the sources. It is interesting that for
appropriately chosen parameters even a Schwarzschild black hole together with a
naked singularity can be "suspended" freely in the superposition of their
fields.Comment: 4 pages; accepted for publication in Phys. Rev.
- …