Not Available

Not AvailableAnthracnose, caused by Colletotrichum spp. is a serious pre- and post-harvest disease in chilli (Capsicum annuum L.) which is a remunerative spice-cum-cash crop of the India. An attempt was made to tag genomic regions controlling anthracnose resistance using reported microsatellite markers.Out of 60 polymorphic SSR markers screened, only four differentiated the individual constituents of resistant and susceptible bulks. Of these four, only one (HpmsE 081) was found associated with genomic regions controlling anthracnose resistance. However, the association was weak as suggested by low contribution of the marker towards the variance of response to anthracnose disease in terms of lesion sizeNot Availabl

Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

Not AvailableDiseases caused by closely related species of Phytophthora such as P. palmivora, P. nicotianae, P. capsici, P. meadii, P. citrophthora and P. colocasiae are major production constraints of plantation crops in India. A reliable method for the rapid identification of these Phytophthora species is required for production of disease-free planting material and promote exports. A specific PCR assay with primer pairs designed from the ITS region effectively differentiated Phytophthora species belonging to different clades viz., P. palmivora, P. nicotianae and P. capsici. In order to differentiate closely related species (P. meadii, P. citrophthora and P. colocasiae), a high-resolution melting (HRM) curve analysis, using primers designed based on single nucleotide differences in A/Tand C/A alleles was developed and validated. HRMcurve analysis proved to be a fast and accurate technique for differentiation of closely related species of Phytophthora