Absolute quantification of viruses by TaqMan real-time RT-PCR in grapevines.

The absolute quantification determines the absolute amount of a targeted nucleic acid expressed as a copy number or concentration. The knowledge of virus concentrations in commercial crops possesses high relevance to ensure a reliable diagnosis. The objective of this study was to perform an absolute quantification of five viruses in infected grapevines (Vitis spp.). Different known amounts of the standard sample (cloned viral cDNA or in vitro transcribed viral RNA) were quantified by TaqMan RT-qPCR. Based on these data, standard curves were generated plotting Ct values (threshold cycle) against the log of the standard sample amount. Infected grapevine samples were evaluated to determine virus titers, which were highly variable. This result may contribute to improve virus diagnosis by accurately quantifying virus titre variations in grapevines. Key words: RT-qPCR, GRSPaV, GVA, GVD, GLRaV-3 and -4. RESUMO: A quantificação absoluta determina a quantidade absoluta de um ácido nucleico alvo expressa como número de cópias ou concentração. O conhecimento das concentrações virais em culturas comerciais tem grande relevância para assegurar um diagnóstico confiável. O objetivo deste trabalho foi realizar uma quantificação absoluta de cinco vírus em videiras infectadas (Vitis spp.). Diferentes quantidades conhecidas da amostra padrão (cDNA viral clonado ou RNA viral transcrito in vitro) foram quantificadas por RT-qPCR TaqMan. A partir destes dados, curvas padrão foram geradas plotando-se os valores de Ct (ciclo limiar) contra o log da quantidade da amostra padrão. Amostras de videiras infectadas foram avaliadas visando-se determinar os títulos virais que foram bastante variáveis. Este resultado contribui para melhorar o diagnóstico viral ao quantificar com precisão variações no título viral em videiras. Palavras-chave: RT-qPCR, GRSPaV, GVA, GVD, GLRaV-3 e -4. A quantificação absoluta determina a quantidade absoluta de um ácido nucleico alvo expressa como número de cópias ou concentração. O conhecimento das concentrações virais em culturas comerciais tem grande relevância para assegurar um diagnóstico confiável. O objetivo deste trabalho foi realizar uma quantificação absoluta de cinco vírus em videiras infectadas (Vitis spp.). Diferentes quantidades conhecidas da amostra padrão (cDNA viral clonado ou RNA viral transcrito in vitro) foram quantificadas por RT-qPCR TaqMan. A partir destes dados, curvas padrão foram geradas plotando-se os valores de Ct (ciclo limiar) contra o log da quantidade da amostra padrão. Amostras de videiras infectadas foram avaliadas visando-se determinar os títulos virais que foram bastante variáveis. Este resultado contribui para melhorar o diagnóstico viral ao quantificar com precisão variações no título viral em videiras. Palavras-chave: RT-qPCR, GRSPaV, GVA, GVD, GLRaV-3 e -4

Exploring efficient imperative handover mechanisms for heterogeneous wireless networks

The Next Generation Internet will provide ubiquitous computing by the seamless operation of heterogeneous wireless networks. It will also provide support for quality-ofservice, QoS, fostering new classes of applications and will havea built-in multi-level security environment. A key requirement of this new infrastructure will be support for efficient vertical handover. Y-Comm is a new architecture that will meet the challenge of this new environment. This paper explores the design of efficient imperative handover mechanisms using the Y-Comm Framework. It first looks at different types of handovers, then examines the Y-Comm Framework and shows how Y-Comm maps unto current mobile infrastructure. It then explores support for different handover mechanisms using Y-Comm. Finally, it highlights the development of a new testbed to further investigate the proposed mechanisms

Expressão de proteína capsídica recombinante do Apple stem pitting virus e produção de antissoro policlonal.

O objetivo deste trabalho foi a expressão do gene da proteína capsidíca do ASPV em bactérias e a produção do antissoro policlonal contra a proteína expressada.Resumo

Dbl oncogene expression in MCF-10 A epithelial cells disrupts mammary acinar architecture, induces EMT and angiogenic factor secretion.

The proteins of the Dbl family are guanine nucleotide exchange factors (GEFs) of Rho GTPases and are known to be involved in cell growth regulation. Alterations of the normal function of these proteins lead to pathological processes such as developmental disorders, neoplastic transformation, and tumor metastasis. We have previously demonstrated that expression of Dbl oncogene in lens epithelial cells modulates genes encoding proteins involved in epithelial-mesenchymal-transition (EMT) and induces angiogenesis in the lens. Our present study was undertaken to investigate the role of Dbl oncogene in epithelial cells transformation, providing new insights into carcinoma progression. To assess how Dbl oncogene can modulate EMT, cell migration, morphogenesis, and expression of pro-apoptotic and angiogenic factors we utilized bi- and three-dimensional cultures of MCF-10░A cells. We show that upon Dbl expression MCF-10░A cells undergo EMT. In addition, we found that Dbl overexpression sustain

Numerical and Experimental Analysis of the Daughter Distribution in Liquid-Liquid Stirred Tanks

The drop size distributions (DSDs) of a dilute immiscible liquid-liquid mixture were measured in a fully turbulent stirred tank operating at different impeller speeds. The results were used to infer the best daughter distribution function (DDF) leading to the best reproduction of the shape of the DSD. Bell-shaped, U-shaped, M-shaped, and uniform statistical DDFs were studied, producing from two to four daughters from each breakup event. A simplified approach from the literature was adopted to solve the population balance equation that considers the spectrum of the turbulence inside the tank obtained from computational fluid dynamics simulations. The U-shaped distribution producing four fragments better reproduces the shape of the experimental DSD in the studied system

Telehealth in oncofertility and breast cancer patients during COVID-19: preliminary results of insenoallasalute.it project

Abstract. – OBJECTIVE: Breast cancer (BC) is the leading diagnosis in premenopausal patients. Lockdown measures during COVID-19 pandemic reduced facilities for premenopausal patients, impairing oncological and reproductive health. To reduce its effect, a telehealth program called insenoallasalute.it was designed in Italy. PATIENTS AND METHODS: A national-based multicentric observational study was undertaken by insenoallasalute.it study group (Italian Ministry of Health, Modena Hospital and Tor Vergata University Hospital) to raise awareness among women on a) BC and its negative role on reproductive health; b) increase adherence to screening programs and self-examination; c) present oncofertility strategies. A webbased platform with two sections was designed: an informative section and a telehealth application activated with a mobile one-time password. After a self-evaluation test to select premenopausal women with maternal desire and family or personal history for BC or ovarian cancer, and premenopausal women with maternity desires with prior medically assisted procreation, a dedicated agenda for telehealth evaluation was displayed and planned. In case the patients fulfilled the criteria for further evaluation, they were invited to perform an outpatient evaluation in one of the pilot centers. RESULTS: From July 2021 to December 2021, 2,830 single accounts were activated, and 2,450 (86.57%) completed the tests. 53 patients were selected to undergo telehealth consultation and 40 (80.0%) scheduled the telehealth visit. 6 patients underwent surgery in the study centers. CONCLUSIONS: In our experience insenoallasalute.it embodied an innovative solution to spread BC awareness, BC screening program, and oncofertility opportunities in the oncological population

A survey of potential architectures for communication in heterogeneous networks

An increasingly wireless world will mean that devices with multiple network interfaces will soon become commonplace. Users will expect to be always connected from anywhere and at any time as connections will be switched to available networks using handover techniques. However, different networks have different Qualities-of-Service so a Quality-of-Service Framework is needed to help applications and services deal with this new environment. In addition, since these networks must work together, future mobile systems will have an open, instead of the currently closed, architecture. Therefore new mechanisms will be needed to protect users, servers and network infrastructure. This means that future mobile networks will have to integrate communications, mobility, quality-of service and security. This paper provides an overview of potential architectures for communication in future networks. Our study shows that only a number of these architectures support this integration

Implementação da técnica de RT-PCR em tempo real para diagnóstico viral no Laboratório de Virologia da Embrapa Uva e Vinho.

Resumo

Detection of three viruses in apples by taqman real time RT-PCR.

Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Resumo 388