Evidence of in situ microbial activity and sulphidogenesis in perennially sub-0 \ub0C and hypersaline sediments of a high Arctic permafrost spring

The lost hammer (LH) spring perennially discharges subzero hypersaline reducing brines through thick layers of permafrost and is the only known terrestrial methane seep in frozen settings on Earth. The present study aimed to identify active microbial communities that populate the sediments of the spring outlet, and verify whether such communities vary seasonally and spatially. Microcosm experiments revealed that the biological reduction of sulfur compounds (SR) with hydrogen (e.g., sulfate reduction) was potentially carried out under combined hypersaline and subzero conditions, down to 1220 \ub0C, the coldest temperature ever recorded for SR. Pyrosequencing analyses of both 16S rRNA (i.e., cDNA) and 16S rRNA genes (i.e., DNA) of sediments retrieved in late winter and summer indicated fairly stable bacterial and archaeal communities at the phylum level. Potentially active bacterial and archaeal communities were dominated by clades related to the T78 Chloroflexi group and Halobacteria species, respectively. The present study indicated that SR, hydrogenotrophy (possibly coupled to autotrophy), and short-chain alkane degradation (other than methane), most likely represent important, previously unaccounted for, metabolic processes carried out by LH microbial communities. Overall, the obtained findings provided additional evidence that the LH system hosts active communities of anaerobic, halophilic, and cryophilic microorganisms despite the extreme conditions in situ.Peer reviewed: YesNRC publication: Ye

Atmospheric Biosignatures

Life has likely coevolved with the Earth system in time in various ways. Our oxygen-rich atmosphere and the protective ozone layer are mainly the result of photosynthetic activity. Additionally, bacteria emit greenhouse gases such as methane and nitrous oxide into the atmosphere, and vegetation can emit a variety of organic molecules. In an exoplanetary context, it is important to consider whether such gas-phase species – so-called atmospheric biosignatures – could be detected spectroscopically and attributed to extraterrestrial life. Another signature of life on Earth is the so-called redox disequilibrium of its atmosphere. This refers to the presence of simultaneously oxidizing and reducing species (e.g., molecular oxygen and methane). Without life, such species would react and be removed on relatively fast timescales. Since Earth’s atmosphere has changed considerably during its history, we will also consider atmospheric biosignatures in the context of the early Earth. This chapter will present a brief literature review of atmospheric biosignatures. We will discuss the main photochemical responses of such species in the modern and early Earth’s atmosphere and their potential to act as atmospheric biosignatures in an exoplanetary context