Alteracija lokomotorne aktivnosti (LA) miševa otrovanih subletalnim dozama olovnog acetata

It was shown that locomotometry allows for continuous recording of some injurious effects of lead poisoning upon mice. For detection of poisoning with lead acetate in mice, locomotometry was more sensitive than the recording of mortality rates, demonstration of stippled cell, in the peripheral blood or detection of manifest symptoms of illness by plain inspection. Single or multiple intraperitoneal injections of saline or distilled water, after the third post-injection day, do not induce detectable alterations of the locomotive activity (LA) of mice. Dose-dependent alterations of LA have been observed after intraperitoneal administration of lead acetate. Acute, single-dose poisoning induces dose-dependent depressions of the LA, while chronic poisoning with multiple injections tends to induce elevated LA, mostly combined with and exceptionally without depression of the LA. Time trends show intermittent alterations of the LA induced by lead poisoning. In some animals significant alterations of LA lasted as long as observed (from 4 to 6 months), while in others the normal LA was restored within 20 or 60 days after the last injection of lead acetate. Animals poisoned with lethal doses of lead acetate developed stippled cells during the 2nd or 3rd week, while significant alterations of the LA started to be manifest from the first day after the first injection. Mice poisoned with subletal doses {0.4, 0.8 and 1.0 mg) showed no stippled cells, but displayed alterations of LA. Possible applications of locomotometry for continuous measurements of degrees of illness or of clinical improvements, in cases of poisoning or of various treatments, were discussed briefly.Metod lokomotometrije omogućuje kontinuirano registrovanje patološkog dejstva otrovanja olovom na miševe. Za detekciju otrovanja olovom metoda lokomotometrije pokazala se je znatno osetljivijom od praćenja mortaliteta, od nalaza bazofilnih granulacija u eritrocitima, i od registrovanja manifestnih simptoma oboljenja. Od trećeg dana posle aplikacije jedne ili više intraperatonealnih injekcija vode, LA miševa ne ispoljava merljive alteracije. Intraperitonealne injekcije rastvora olovnog acetata izazivaju alteraciju LA, koja je proporcionalna dozi unetog otrova. Akutna otrovanja, izazvana pojedinačnim dozama izazivaju usporavanje pokretljivosti, dok je u slučajevima hroničnih otrovanja, izazvanih multiplim dozama olova, zapažene i povišenje LA miševa. U nekih životinja zapažane su mesecima značajne, većinom intermibirajuće, aliteracije LA, dok je u drugih LA normalizovana u roku od 20 do 60 dana posle poslednje injekcije olovnog acetata. Bazofilne granulacije eritrocita pojavljuju se tek oko desetog dana posle otrovanja letalnim dozama olova, dok je značajna alteraoija LA zapažena već prvih dana posle davanja otrova. U eritrocitima miševa otrovanih subletainim dozama otrova bazofilne granulacije nisu nađene. Diskutovane su mogućnosti primene lokomotometrije u cilju kontinuiranog merenja jačine bolesti usled otrovanja ili u cilju ocene kliničkog poboljšanja pod dejstvom raznih tretmana