Effects of recombinant IFN-α2b on the phenotype of neutrophil granulocyte subpopulations in patients with COVID-19

Investigation of molecular mechanisms associated with interferon (IFN) production and receptor function of neutrophil granulocytes (NGs) in COVID-19 is highly relevant because it can be promising in the search for new therapeutic strategies targeting NGs and their reactivity to restore and strengthen the innate immune response against SARS-CoV-2. Objective. To assess the effects of recombinant IFN-α2b on the phenotype of CD16+IFNα/βR1–CD119+, CD16+IFNα/βR1+CD119–, and CD16+IFNα/βR1+CD119+ NGs from peripheral blood of patients with COVID-19 in an in vitro experiment. Patients and methods. We analyzed blood samples from 31 patients with a mean age of 61 years (range: 57;71 years) with moderate COVID-19. We assessed the number of CD16+IFNα/βR1–CD119+, CD16+IFNα/βR1+CD119–, and CD16+IFNα/βR1+CD119+ NGs, receptor density (FC 500, ‘Beckman Coulter,’ USA), phagocytic activity of NGs before and after incubation with recombinant IFN-α2b. We also measured serum levels of several cytokines, including IFNα, IFNγ, IL-6, IL-8 (ELISA, ‘Vektor-Best’ LLC). The control group comprised 22 adult healthy individuals with a mean age of 58 years (range: 57; 70 years). Results. Patients with moderate COVID-19 demonstrated low serum levels of IFNα and IFNγ along with elevated levels of IL-6 and IL-8. We observed transformation of 3 phenotypes among NG subpopulations: CD16+IFNα/βR1–CD119+, CD16+IFNα/βR1+CD119-, and CD16+IFNα/βR1+CD119+. We observed positive remodulating effects of recombinant IFN-α2b on the number and phenotype of NG subpopulations and their phagocytic activity in our in vitro experiment. Conclusion. Recombinant IFN-α2b demonstrated positive effects in in vitro experiments; therefore, it can be considered in the future as a potential therapeutic tool for moderate COVID-19. Restoration of type I IFN might be an effective treatment option for COVID-19, because it can promote faster virus elimination, restore normal functioning of the IFN system, and have positive regulatory effects on the phenotype of NG subpopulations. © 2022, Dynasty Publishing House. All rights reserved

Improving the quality of ShKh15 ball bearing steel by vibrotreating the ingots

15.00; Translated from Russian (Stal' 1987 (4) p. 48-49)SIGLEAvailable from British Library Document Supply Centre- DSC:9022.06(BISI--26012)T / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Comparative analysis of terms of participation of Ukraine is in world organization of trade with other members of multilateral auction system (2012 – 2013)

У статті наведені результати порівняльного аналізу умов участі України в Світовій організації торгівлі із іншими членами багатосторонньої торгівельної системи та розкриті перспективні напрямки подальшого розвитку зовнішньоторговельної діяльності України.В статье приведены результаты сравнительного анализа условий участия Украины в Мировой организации торговли с другими членами многосторонней торговой системы и раскрыты перспективные направления последующего развития внешнеторговой деятельности Украины.In the article the results of comparative analysis of terms of participation of Ukraine are resulted in World organization of trade with other members of the multilateral auction system and perspective directions of subsequent development foreign trade activity of Ukraine are exposed