6 research outputs found
ΠΡΠΈΡΠΈΠ½Ρ Π»ΠΎΠΆΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΈΠΎΠ·ΠΈΡΠ° Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΡΡΠ΅ΡΠ»ΠΈΠ½ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ: ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ»ΡΡΠ°ΠΉ
Differential diagnosis of inflammatory myopathies with hereditary muscular dystrophies accompanied by a secondary inflammatory process is a timeβconsuming clinical and pathomorphological task. In particular, false diagnosis of polymyositis in patients with dysferlinopathy reaches 25 % of cases.A 40βyearβold female patient with a limbβgirdle phenotype of dysferlinopathy, initially diagnosed as polymyositis, is presented. The reasons that led to the erroneous diagnosis were: sporadic case; subacute onset; proximal muscle weakness; myalgia, which stopped on the glucocorticosteroid therapy; high levels of creatine phosphokinase (up to 17 times); the presence of lymphocyticβmacrophage infiltrate in the muscle biopsy and the absence of magnetic resonance imaging data in primary examination of the patient.The refractoriness of clinical and laboratory signs to complex immunosuppressive therapy was the reason for revising the muscle biopsy with typing of the inflammatory infiltrate. The predominantly unexpressed perivascular infiltrate was characterized by the predominance of macrophages and, to a lesser extent, CD4+, which indicated the secondary nature of the inflammation in the muscle observed in some hereditary muscular dystrophies. When conducting an immunohistochemical reaction, the absence of the dysferlin protein in the sarcoplasmic membrane was revealed.Wholeβexome sequencing (NGS) revealed a mutation in exon 39 of the DYSF gene (p.Gln1428Ter) in the heterozygous state, which leads to the appearance of a stop codon and premature termination of protein translation. MLPA method registered 3 copies of exons 18, 19, 20, 22, 24 of the DYSF gene.Thus, this clinical example reflects the main methodological errors and possible effects of immunosuppressive therapy in patients with dysferlinopathy.ΠΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½Π°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΌΠΈΠΎΠΏΠ°ΡΠΈΠΉ, ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΠΈΡ
ΡΡ Π²ΡΠΎΡΠΈΡΠ½ΡΠΌ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠΌ, Ρ Π½Π°ΡΠ»Π΅Π΄ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ ΠΌΡΡΠ΅ΡΠ½ΡΠΌΠΈ Π΄ΠΈΡΡΡΠΎΡΠΈΡΠΌΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ»ΠΎΠΆΠ½ΠΎΠΉ ΠΈ ΡΡΡΠ΄ΠΎΠ΅ΠΌΠΊΠΎΠΉ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎβΠΏΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π·Π°Π΄Π°ΡΠ΅ΠΉ. Π ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ, Π»ΠΎΠΆΠ½Π°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΠΏΠΎΠ»ΠΈΠΌΠΈΠΎΠ·ΠΈΡΠ° Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΡΡΠ΅ΡΠ»ΠΈΠ½ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ Π΄ΠΎΡΡΠΈΠ³Π°Π΅Ρ 25 % ΡΠ»ΡΡΠ°Π΅Π².ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π° ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΊΠ° 40 Π»Π΅Ρ Ρ ΠΏΠΎΡΡΠ½ΠΎβΠΊΠΎΠ½Π΅ΡΠ½ΠΎΡΡΠ½ΡΠΌ ΡΠ΅Π½ΠΎΡΠΈΠΏΠΎΠΌ Π΄ΠΈΡΡΠ΅ΡΠ»ΠΈΠ½ΠΎΠΏΠ°ΡΠΈΠΈ, ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΠΊΠ°ΠΊ ΠΏΠΎΠ»ΠΈΠΌΠΈΠΎΠ·ΠΈΡ. ΠΡΠΈΡΠΈΠ½Ρ, ΠΏΠΎΠ²Π»Π΅ΠΊΡΠΈΠ΅ ΠΎΡΠΈΠ±ΠΎΡΠ½ΡΡ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΡ: ΡΠΏΠΎΡΠ°Π΄ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠ΅; ΠΏΠΎΠ΄ΠΎΡΡΡΡΠΉ Π΄Π΅Π±ΡΡ; ΠΏΡΠΎΠΊΡΠΈΠΌΠ°Π»ΡΠ½Π°Ρ ΠΌΡΡΠ΅ΡΠ½Π°Ρ ΡΠ»Π°Π±ΠΎΡΡΡ; ΠΌΠΈΠ°Π»Π³ΠΈΡ, ΠΊΡΠΏΠΈΡΠΎΠ²Π°Π²ΡΠ°ΡΡΡ Π½Π° ΡΠΎΠ½Π΅ Π³Π»ΡΠΊΠΎΠΊΠΎΡΡΠΈΠΊΠΎΡΡΠ΅ΡΠΎΠΈΠ΄Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ; ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ ΠΊΡΠ΅Π°ΡΠΈΠ½ΡΠΎΡΡΠΎΠΊΠΈΠ½Π°Π·Ρ (Π΄ΠΎ 17 ΡΠ°Π·); Π½Π°Π»ΠΈΡΠΈΠ΅ Π»ΠΈΠΌΡΠΎΡΠΈΡΠ°ΡΠ½ΠΎβΠΌΠ°ΠΊΡΠΎΡΠ°Π³Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ° Π² ΠΌΡΡΠ΅ΡΠ½ΠΎΠΌ Π±ΠΈΠΎΠΏΡΠ°ΡΠ΅ ΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ Π΄Π°Π½Π½ΡΡ
ΠΌΠ°Π³Π½ΠΈΡΠ½ΠΎβΡΠ΅Π·ΠΎΠ½Π°Π½ΡΠ½ΠΎΠΉ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΏΡΠΈ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΌ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ.Π Π΅ΡΡΠ°ΠΊΡΠ΅ΡΠ½ΠΎΡΡΡ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎβΠ»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ² ΠΊ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠΉ ΠΈΠΌΠΌΡΠ½ΠΎΡΡΠΏΡΠ΅ΡΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΠΎΡΠ»ΡΠΆΠΈΠ»Π° ΠΏΡΠΈΡΠΈΠ½ΠΎΠΉ ΠΏΠ΅ΡΠ΅ΡΠΌΠΎΡΡΠ° ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π±ΠΈΠΎΠΏΡΠΈΠΈ ΠΌΡΡΡΡ Ρ ΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠ°. ΠΠ΅Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΉ, ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΏΠ΅ΡΠΈΠ²Π°ΡΠΊΡΠ»ΡΡΠ½ΡΠΉ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π»ΡΡ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΈ, Π² ΠΌΠ΅Π½ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ, CD4+, ΡΡΠΎ ΡΠΊΠ°Π·ΡΠ²Π°Π»ΠΎ Π½Π° Π²ΡΠΎΡΠΈΡΠ½ΡΠΉ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΡ Π² ΠΌΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, Π½Π°Π±Π»ΡΠ΄Π°Π΅ΠΌΠΎΠ³ΠΎ ΠΏΡΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Π½Π°ΡΠ»Π΅Π΄ΡΡΠ²Π΅Π½Π½ΡΡ
ΠΌΡΡΠ΅ΡΠ½ΡΡ
Π΄ΠΈΡΡΡΠΎΡΠΈΡΡ
. ΠΡΠΈ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π²ΡΡΠ²Π»Π΅Π½ΠΎ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ Π±Π΅Π»ΠΊΠ° Π΄ΠΈΡΡΠ΅ΡΠ»ΠΈΠ½Π° Π² ΡΠ°ΡΠΊΠΎΠΏΠ»Π°Π·ΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΌΠ΅ΠΌΠ±ΡΠ°Π½Π΅.Π Ρ
ΠΎΠ΄Π΅ ΠΏΠΎΠ»Π½ΠΎΡΠΊΠ·ΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ (NGS) Π²ΡΡΠ²Π»Π΅Π½Π° ΠΌΡΡΠ°ΡΠΈΡ Π² 39βΠΌ ΡΠΊΠ·ΠΎΠ½Π΅ Π³Π΅Π½Π° DYSF (p.Gln1428Ter) Π² Π³Π΅ΡΠ΅ΡΠΎΠ·ΠΈΠ³ΠΎΡΠ½ΠΎΠΌ ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠ°Ρ ΠΊ ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΡ ΡΡΠΎΠΏβΠΊΠΎΠ΄ΠΎΠ½Π° ΠΈ ΠΏΡΠ΅ΠΆΠ΄Π΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΠ΅ΡΠΌΠΈΠ½Π°ΡΠΈΠΈ ΡΡΠ°Π½ΡΠ»ΡΡΠΈΠΈ Π±Π΅Π»ΠΊΠ°. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ MLPA Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΠΏΠΎ 3 ΠΊΠΎΠΏΠΈΠΈ 18, 19, 20, 22, 24βΠ³ΠΎ ΡΠΊΠ·ΠΎΠ½ΠΎΠ² Π³Π΅Π½Π° DYSF. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠ»ΡΡΠ°ΠΉ ΠΎΡΡΠ°ΠΆΠ°Π΅Ρ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΎΡΠΈΠ±ΠΊΠΈ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΡΠΏΡΠ΅ΡΡΠΈΠ²Π½ΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΡΡΠ΅ΡΠ»ΠΈΠ½ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ
ΠΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠΈ ΡΠ°Π½Π½Π΅ΠΉ ΡΠΏΠΈΠ»Π΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠΈ 66βΠ³ΠΎ ΡΠΈΠΏΠ° (ΠΎΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ ΠΈ ΡΠΎΠ±ΡΡΠ²Π΅Π½Π½ΠΎΠ΅ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΠ΅)
Early epileptic encephalopathy-66 was first diagnosed in a male patient from Russia using whole-exome sequencing. Early epileptic encephalopathy- 66 is a unique disorder in the group of early epileptic encephalopathies. The same recurrent heterozygous variant of the nucleotide sequence was found in all known patients, but the severity of seizures and dysmorphic signs significantly vary between patients. The current study of a recurrent pathogenic variant in PACS2 gene expands the phenotype spectrum of early epileptic encephalopathy-66 and will improve the management of patients with that disorder in Russia in the future.ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ΠΎ ΠΏΠ΅ΡΠ²ΠΎΠ΅ ΠΎΠΏΠΈΡΠ°Π½ΠΈΠ΅ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊ ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ Π±ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ Ρ ΡΠ°Π½Π½Π΅ΠΉ ΡΠΏΠΈΠ»Π΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠ΅ΠΉ 66βΠ³ΠΎ ΡΠΈΠΏΠ°. Π‘ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΠΎΠ»Π½ΠΎΡΠΊΠ·ΠΎΠΌΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½Π° ΡΠ°Π½Π΅Π΅ ΠΎΠΏΠΈΡΠ°Π½Π½Π°Ρ Π³Π΅ΡΠ΅ΡΠΎΠ·ΠΈΠ³ΠΎΡΠ½Π°Ρ ΠΌΡΡΠ°ΡΠΈΡ NM_001100913.2: c.625G>A (p.Glu209Lys) Π² Π³Π΅Π½Π΅ PACS2. ΠΠ°Π½Π½ΠΎΠ΅ ΠΌΠΎΠ½ΠΎΠ³Π΅Π½Π½ΠΎΠ΅ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠ΅ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ½ΠΈΠΊΠ°Π»ΡΠ½ΡΠΌ Π² Π³ΡΡΠΏΠΏΠ΅ ΡΠ°Π½Π½ΠΈΡ
ΡΠΏΠΈΠ»Π΅ΠΏΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΠΉ β Ρ Π²ΡΠ΅Ρ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΎΠ±Π½Π°ΡΡΠΆΠΈΠ²Π°Π΅ΡΡΡ ΠΎΠ΄ΠΈΠ½Π°ΠΊΠΎΠ²ΡΠΉ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΠΉ Π²Π°ΡΠΈΠ°Π½Ρ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ, Π½ΠΎ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ ΠΎΡΠ»ΠΈΡΠ°ΡΡΡΡ ΠΏΠΎ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΡΠΆΠ΅ΡΡΠΈ ΠΈ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π΄ΠΈΠ·ΠΌΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ², ΡΡΠΎ ΠΏΡΠ΅Π΄- ΠΏΠΎΠ»ΠΎΠΆΠΈΡΠ΅Π»ΡΠ½ΠΎ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠ°Π·Π½ΡΠΌ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠΎΠ½ΠΎΠΌ. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΅ΡΠΈΠΉ ΡΠ»ΡΡΠ°Π΅Π² ΡΠ΅ΠΊΡΡΡΠ΅Π½ΡΠ½ΡΡ
ΠΏΠ°ΡΠΎΠ³Π΅Π½Π½ΡΡ
Π²Π°ΡΠΈΠ°Π½ΡΠΎΠ² ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΠΎΠΏΡΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ°ΠΊΡΠΈΠΊΡ Π²Π΅Π΄Π΅Π½ΠΈΡ Π½ΠΎΠ²ΡΡ
ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΏΡΠΈ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΈΠΈ ΡΠΆΠ΅ ΠΈΠ·Π²Π΅ΡΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²Π°ΡΠΈΠ°Π½ΡΠ°
Clinical and genetic characteristics of the early 66th type epileptic encephalopathy (literature review and own observation)
Early epileptic encephalopathy-66 was first diagnosed in a male patient from Russia using whole-exome sequencing. Early epileptic encephalopathy- 66 is a unique disorder in the group of early epileptic encephalopathies. The same recurrent heterozygous variant of the nucleotide sequence was found in all known patients, but the severity of seizures and dysmorphic signs significantly vary between patients. The current study of a recurrent pathogenic variant in PACS2 gene expands the phenotype spectrum of early epileptic encephalopathy-66 and will improve the management of patients with that disorder in Russia in the future
Genetic screening of an endemic mutation in the DYSF gene in an isolated, mountainous population in the Republic of Dagestan
Abstract Background Dysferlinopathy has a high prevalence in relatively isolated ethnic groups where consanguineous marriages are characteristic and/or the founder effect exists. However, the frequency of endemic mutations in most isolates has not been investigated. Methods The prevalence of the pathological DYSF gene variant (NM_003494.4); c.200_201delinsAT, p. Val67Asp (rs121908957) was investigated in an isolated Avar population in the Republic of Dagestan. Genetic screenings were conducted in a remote mountainous region characterized by a high level of consanguinity among its inhabitants. In total, 746 individuals were included in the screenings. Results This pathological DYSF gene variant causes two primary phenotypes of dysferlinopathy: limbβgirdle muscular dystrophy (LGMD) type R2 and Miyoshi muscular dystrophy type 1. Results indicated a high prevalence of the allele at 14% (95% confidence interval [CI]: 12β17; 138 out of 1518 alleles), while the allele in the homozygous state was detected in 29 casesβ3.8% (CI: 2.6β5.4). The population load for dysferlinopathy was 832.3βΒ±β153.9 per 100,000 with an average prevalence of limbβgirdle muscular dystrophies ranging from 0.38βΒ±β0.38 to 5.93βΒ±β1.44 per 100,000. Conclusion A significant burden of the allele was due to inbreeding, as evidenced by a deficiency of heterozygotes and the Wright fixation index equal to 0.14 (CI 0.06β0.23)
Biallelic variants in PPP1R13L cause paediatric dilated cardiomyopathy
Childhood dilated cardiomyopathy (DCM) is a leading cause of heart failure requiring cardiac transplantation and approximately 5% of cases result in sudden death. Knowledge of the underlying genetic cause can aid prognostication and clinical management and enables accurate recurrence risk counselling for the family. Here we used genomic sequencing to identify the causative genetic variant(s) in families with children affected by severe DCM. In an international collaborative effort facilitated by GeneMatcher, biallelic variants in PPP1R13L were identified in seven children with severe DCM from five unrelated families following exome or genome sequencing and inheritance-based variant filtering. PPP1R13L encodes inhibitor of apoptosis-stimulating protein of p53 protein (iASPP). In addition to roles in apoptosis, iASPP acts as a regulator of desmosomes and has been implicated in inflammatory pathways. DCM presented early (mean: 2?years 10?months; range: 3?months-9?years) and was progressive, resulting in death (n = 3) or transplant (n = 3), with one child currently awaiting transplant. Genomic sequencing technologies are valuable for the identification of novel and emerging candidate genes. Biallelic variants in PPP1R13L were previously reported in a single consanguineous family with paediatric DCM. The identification here of a further five families now provides sufficient evidence to support a robust gene-disease association between PPP1R13L and severe paediatric DCM. The PPP1R13L gene should be included in panel-based genetic testing for paediatric DCM.The article is available via Open Access. Click on the 'Additional link' above to access the full-text.Published version, accepted version, submitted versio