9 research outputs found
ΠΠ½Π΄ΡΠΊΡΠΈΡ ΠΎΡΡΠ΅ΠΎΠ³Π΅Π½Π΅Π·Π° ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎβΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π³ΡΠ±ΡΠ°ΡΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ 3D-Π½ΠΎΡΠΈΡΠ΅Π»Ρ, Π½Π°Π³ΡΡΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠΌ
Objective: to study the induction of osteogenesis caused by introducing into the defect area broadly porous cryogenically structured 3D carriers, based on serum albumin and loaded with a bioregulator isolated from bovine serum on an experimental model of mandible defect in rabbits in vivo.Materials and methods. Cryogenically structured sponges in the form of cylindrical specimens, 5 mm in diameter and 5 mm in height, prepared from bovine serum albumin, were used as the bioregulator carrier. The experimental laboratory animals were male Chinchilla rabbits, weighing 2β2.5 kg. Bone tissue was skeletonized under anesthesia (intramuscular anesthetic Zoletil 100) with a 3-cm incision in the angle of the mandible and a 5-mm-diameter cutter was used to create a 2β3-mm deep defect to install an appropriate-size albumin sponge. A total of 24 animals participated in the experiment. X-ray control of the defect area was performed in vivo on day 14 using PanExam+ (Kavo) device (20 m X-ray). Histological examination of tissues was carried out at day 30 after the defect using a light microscope.Results. Experiments performed indicate an active restoration of bone tissue in the extensive defect area when using an albumin-based 3D carrier with the inclusion of a bioregulator as compared to the control experiments. There were osteointegrative and osteoinductive processes, almost complete decomposition (biodegradation) of albumin sponge with formation of islands of dense bone tissue with small foci of coarse fibrous tissue in the defect. This demonstrated good dynamics of recovery processes at this stage of healing.Conclusion. Under the action of a serum bioregulator contained in an albumin-based sponge, the repair process leads to restoration of normal bone tissue without formation of bone callus and altered bone tissue different from the native one.Π¦Π΅Π»Ρ ΡΠ°Π±ΠΎΡΡ: ΠΈΠ·ΡΡΠΈΡΡ in vivo Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΠΊΡΠΎΠ»ΠΈΠΊΠ° ΠΈΠ½Π΄ΡΠΊΡΠΈΡ ΠΎΡΡΠ΅ΠΎΠ³Π΅Π½Π΅Π·Π°, Π²ΡΠ·ΡΠ²Π°Π΅ΠΌΡΡ Π²Π½Π΅ΡΠ΅Π½ΠΈΠ΅ΠΌ Π² ΠΎΠ±Π»Π°ΡΡΡ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΡΠΈΡΠΎΠΊΠΎΠΏΠΎΡΠΈΡΡΡΡ
ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎ-ΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
3D-Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π°, Π½Π°Π³ΡΡΠΆΠ΅Π½Π½ΡΡ
Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠΎΠΌ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΠΌ ΠΈΠ· ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ ΠΊΡΡΠΏΠ½ΠΎΠ³ΠΎ ΡΠΎΠ³Π°ΡΠΎΠ³ΠΎ ΡΠΊΠΎΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ Π½ΠΎΡΠΈΡΠ΅Π»Ρ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ ΠΊΡΠΈΠΎΠ³Π΅Π½Π½ΠΎ-ΡΡΡΡΠΊΡΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Π³ΡΠ±ΠΊΠΈ Π² Π²ΠΈΠ΄Π΅ ΡΠΈΠ»ΠΈΠ½Π΄ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ 5 ΠΌΠΌ ΠΈ Π²ΡΡΠΎΡΠΎΠΉ ΡΠ°ΠΊΠΆΠ΅ 5 ΠΌΠΌ, ΠΏΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΈΠ· Π±ΡΡΡΠ΅Π³ΠΎ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π°Π»ΡΠ±ΡΠΌΠΈΠ½Π°. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΡ Ρ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° ΠΊΡΠΎΠ»ΠΈΠΊΠ°Ρ
ΠΏΠΎΡΠΎΠ΄Ρ Π¨ΠΈΠ½ΡΠΈΠ»Π»Π° Π²Π΅ΡΠΎΠΌ 2β2,5 ΠΊΠ³, ΡΠ°ΠΌΡΠ°Ρ
. ΠΠΎΠ΄ Π½Π°ΡΠΊΠΎΠ·ΠΎΠΌ (Π²Π½ΡΡΡΠΈΠΌΡΡΠ΅ΡΠ½ΡΠΉ Π½Π°ΡΠΊΠΎΠ· Zoletil 100) ΡΠ°Π·ΡΠ΅Π·ΠΎΠΌ Π΄ΠΎ 3 ΡΠΌ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ³Π»Π° Π½ΠΈΠΆΠ½Π΅ΠΉ ΡΠ΅Π»ΡΡΡΠΈ ΡΠΊΠ΅Π»Π΅ΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΊΠΎΡΡΠ½ΡΡ ΡΠΊΠ°Π½Ρ ΠΈ ΡΡΠ΅Π·ΠΎΠΉ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ 5 ΠΌΠΌ ΡΠΎΠ·Π΄Π°Π²Π°Π»ΠΈ Π΄Π΅ΡΠ΅ΠΊΡ Π³Π»ΡΠ±ΠΈΠ½ΠΎΠΉ 2β3 ΠΌΠΌ Π΄Π»Ρ ΡΡΡΠ°Π½ΠΎΠ²ΠΊΠΈ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ ΡΠ°Π·ΠΌΠ΅ΡΠ°. ΠΡΠ΅Π³ΠΎ Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ΅ ΠΏΡΠΈΡΡΡΡΡΠ²ΠΎΠ²Π°Π»ΠΎ 24 ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. ΠΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΠ΅Π½ΡΠ³Π΅Π½ΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΠΎΠ±Π»Π°ΡΡΠΈ Π΄Π΅ΡΠ΅ΠΊΡΠ° Π½Π° 14-Π΅ ΡΡΡΠΊΠΈ ΠΏΡΠΈΠΆΠΈΠ·Π½Π΅Π½Π½ΠΎ Π½Π° Π°ΠΏΠΏΠ°ΡΠ°ΡΠ΅ PanExam+ (Kavo), 20 ΠΌΠ Π΅Π½ΡΠ³Π΅Π½. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠΊΠ°Π½Π΅ΠΉ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π½Π° 30-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ Π½Π°Π½Π΅ΡΠ΅Π½ΠΈΡ Π΄Π΅ΡΠ΅ΠΊΡΠ° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ
ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠΎΠ² ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎΠ± Π°ΠΊΡΠΈΠ²Π½ΠΎΠΌ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠΈ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π² ΠΎΠ±Π»Π°ΡΡΠΈ ΠΎΠ±ΡΠΈΡΠ½ΠΎΠ³ΠΎ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ 3D-Π½ΠΎΡΠΈΡΠ΅Π»Ρ ΠΈΠΌΠ΅Π½Π½ΠΎ Ρ Π²ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ ΠΎΠΏΡΡΠ°ΠΌΠΈ. ΠΠ°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½Ρ ΠΏΡΠΎΡΠ΅ΡΡΡ ΠΎΡΡΠ΅ΠΎΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ ΠΈ ΠΎΡΡΠ΅ΠΎΠΈΠ½Π΄ΡΠΊΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ, ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ ΠΏΠΎΠ»Π½ΠΎΠ΅ ΡΠ°Π·Π»ΠΎΠΆΠ΅Π½ΠΈΠ΅ (Π±ΠΈΠΎΠ΄Π΅Π³ΡΠ°Π΄Π°ΡΠΈΡ) Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π½Π° ΠΌΠ΅ΡΡΠ΅ Π΄Π΅ΡΠ΅ΠΊΡΠ° ΠΎΡΡΡΠΎΠ²ΠΊΠΎΠ² ΠΏΠ»ΠΎΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Ρ Π½Π΅Π±ΠΎΠ»ΡΡΠΈΠΌΠΈ ΠΎΡΠ°Π³Π°ΠΌΠΈ Π³ΡΡΠ±ΠΎΠ²ΠΎΠ»ΠΎΠΊΠ½ΠΈΡΡΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΡΡΠΎ Π³ΠΎΠ²ΠΎΡΠΈΡ ΠΎ Ρ
ΠΎΡΠΎΡΠ΅ΠΉ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² Π½Π° Π΄Π°Π½Π½ΠΎΠΌ ΡΡΠΎΠΊΠ΅ Π·Π°ΠΆΠΈΠ²Π»Π΅Π½ΠΈΡ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΏΠΎΠ΄ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΡΡΠ²ΠΎΡΠΎΡΠΎΡΠ½ΠΎΠ³ΠΎ Π±ΠΈΠΎΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ° Π² ΡΠΎΡΡΠ°Π²Π΅ Π°Π»ΡΠ±ΡΠΌΠΈΠ½ΠΎΠ²ΠΎΠΉ Π³ΡΠ±ΠΊΠΈ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ Π±Π΅Π· ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΠΌΠΎΠ·ΠΎΠ»ΠΈ ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½Π½ΠΎΠΉ ΠΊΠΎΡΡΠ½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ, ΠΎΡΠ»ΠΈΡΠ½ΠΎΠΉ ΠΎΡ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ
Changes in human sperm motility characteristics in the presence of protein-peptide complex
The study objective is to examine the changes in motility of human spermatozoa under the effect of a protein-peptide complex extracted from cattle testes (Bos taurus).Materials and methods. The protein-peptide complex was extracted from testes tissue obtained during butchering of cattle at meat processing plants of the Moscow Region. Human spermatozoa were obtained from the ejaculate in the usual way. From the ejaculate sample, 1 ml was collected for the experiment and control. The protein-peptide complex was added to a test tube in concentrations of 10β8, 10β9, andΒ 10β12 mg/ml. For every concentration, 10β12 experiments were performed. General and active motility were evaluated after 30 min, 1 and 3 hours of incubation at room temperature.Results and conclusion. The experiments have shown that in the presence of the protein-peptide complex, spermatozoa motility increasedΒ by 10β30 % relative to the initial value at 30 minutes, and this change persisted for 3 hours of observation. The change depended on the complex concentration: the largest increase was observed at 10β12 mg/ml. Higher complex concentrations did not have a positive effectΒ on spermatozoa motility