328 research outputs found

    High Q Shielded Dielectric Resonator -- The Basis of Stable MM-Waves Generator

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    High Q-factor whispering gallery modes which are excited in a shielded hemispherical dielectric resonator by an external signal or Gunn diode are investigated. Their application as modes of an oscillator with high frequency stability is proposed. The power of the output signal and frequency tuning of an oscillator based on the investigated resonator are measured

    Widespread presence of "bacterial-like" PPP phosphatases in eukaryotes

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    BACKGROUND: In eukaryotes, PPP (protein phosphatase P) family is one of the two known protein phosphatase families specific for Ser and Thr. The role of PPP phosphatases in multiple signaling pathways in eukaryotic cell has been extensively studied. Unlike eukaryotic PPP phosphatases, bacterial members of the family have broad substrate specificity or may even be Tyr-specific. Moreover, one group of bacterial PPPs are diadenosine tetraphosphatases, indicating that bacterial PPP phosphatases may not necessarily function as protein phosphatases. RESULTS: We describe the presence in eukaryotes of three groups of expressed genes encoding "non-conventional" phosphatases of the PPP family. These enzymes are more closely related to bacterial PPP phosphatases than to the known eukaryotic members of the family. One group, found exclusively in land plants, is most closely related to PPP phosphatases from some α-Proteobacteria, including Rhizobiales, Rhodobacterales and Rhodospirillaceae. This group is therefore termed Rhizobiales / Rhodobacterales / Rhodospirillaceae-like phosphatases, or Rhilphs. Phosphatases of the other group are found in Viridiplantae, Rhodophyta, Trypanosomatidae, Plasmodium and some fungi. They are structurally related to phosphatases from psychrophilic bacteria Shewanella and Colwellia, and are termed Shewanella-like phosphatases, or Shelphs. Phosphatases of the third group are distantly related to ApaH, bacterial diadenosine tetraphosphatases, and are termed ApaH-like phosphatases, or Alphs. Patchy distribution of Alphs in animals, plants, fungi, diatoms and kinetoplasts suggests that these phosphatases were present in the common ancestor of eukaryotes but were independently lost in many lineages. Rhilphs, Shelphs and Alphs form PPP clades, as divergent from "conventional" eukaryotic PPP phosphatases as they are from each other and from major bacterial clades. In addition, comparison of primary structures revealed a previously unrecognised (I/L/V)D(S/T)G motif, conserved in all bacterial and "bacterial-like" eukaryotic PPPs, but not in "conventional" eukaryotic and archaeal PPPs. CONCLUSIONS: Our findings demonstrate that many eukaryotes possess diverse "bacterial-like" PPP phosphatases, the enzymatic characteristics, physiological roles and precise evolutionary history of which have yet to be determined

    Excitation of the High-Q-factor Whispering Gallery Modes in the Hemispherical Dielectric Resonator with the Cylinder Shield

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    A hollow metal cylinder with a semicircle in the base is used as the shield of a hemispherical dielectric resonator with whispering gallery (WG) modes. It is shown that the spectrum of the WG modes of this resonator is clearer than the spectrum of a similar resonator with a hemispherical shield. It is determined that the high-Q-factor WG modes are excited in the proposed resonator by the coupling slot on the mirror. Their fields are localized in the dielectric. The frequency dependence of the Q-factor value is measured. A good conformity of this experimental dependence with the analytic dependence is obtained in this pape

    Millimeter Wave Oscillator Based Dielectric Hemisphere with Cylinder Shield

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    The purpose of this paper is the investigation of the main Gunn diode oscillator characteristics with a hemispherical dielectric resonator which is shielded by a metal cylinder. The frequency, output power and electronic tuning of frequency, the steepness of which allows one to estimate the frequency stability in relation to the parasitic change of the Gunn diode voltage, and their comparative analysis with the characteristics of generation in a similar open dielectric resonator
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