Synthesis of substance P partial sequential peptides on a photolytically removable 4-aminomethyl-3-nitrobenzoylaminomethyl polystyrene support

Sequential peptides corresponding to substance P (6–11) were synthesised on a photocleavable 4-aminomethyl-3-nitrobenzoylaminomethyl polystyrene resin. This photoremovable polymeric support, useful for the synthesis of C-terminal peptide amides, was prepared from 4-bromomethyl-3-nitrobenzoylaminomethyl polystyrene by treatment with potassium phthalimide followed by hydrazinolysis. Amino acid residues were incorporated onto this support following the standard solid-phase methodology of peptide synthesis. The protected peptide amides were cleaved from the resin by photolysis with light of wavelength 350 nm. The resin was used for the synthesis of peptides including the partial sequence corresponding to substance P (9-11) which was further subjected to stepwise synthesis to prepare substance P (6-11) in an overall yield of 88%. This approach combines the advantages of the photochemical deprotection of the 4-aminomethyl-3-nitro carboxyl protecting group, the polymer-supported peptide synthesis and the polymer-analogous functionalisation procedure to obtain the C-terminal peptide amide under mild conditions

Polymer-bound mixed carboxylic dithiocarbamic anhydrides. A new class of selective and recyclable solid-phase acylating reagents

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Cystic lymphangioma of pericardium presenting as isolated chylopericardium – A case report

Isolated chylopericardium due to cystic lymphangioma of pericardium is a rare entity. We report a case of asymptomatic chylopericardium in a young male who presented with cardiomegaly. Echocardiography revealed massive pericardial effusion without tamponade. Pericardiocentesis yielded 1.25 L of tea-colored fluid which showed triglyceride level of 1723 mg/dL and cholesterol of 1021 mg/dL with a cholesterol to triglyceride ratio of <1, characteristic of chylous fluid. Lymphoscintigraphy using 99Tc demonstrated lymphatic leak around the heart region. Fusion of MRI images with lymphoscintigraphy was taken with a view of localizing the leak site; it demonstrated enhancement in the pericardial space. Surgery was done via right lateral thoracotomy. Thoracic duct was ligated above diaphragm and pericardial window created by anterior pericardiectomy. The patient had an uneventful recovery and was well after 6 months of follow up. Pericardial biopsy showed cystic lymphangioma of pericardium