Development and Validation of Diagnostic KASP Markers for Brown Planthopper Resistance in Rice

Rice (Oryza sativa L.) is an important source of nutrition for the world’s burgeoning population that often faces yield loss due to infestation by the brown planthopper (BPH, Nilaparvata lugens (Stål)). The development of rice cultivars with BPH resistance is one of the crucial precedences in rice breeding programs. Recent progress in high-throughput SNP-based genotyping technology has made it possible to develop markers linked to the BPH more quickly than ever before. With this view, a genome-wide association study was undertaken for deriving marker-trait associations with BPH damage scores and SNPs from genotyping-by-sequencing data of 391 multi-parent advanced generation inter-cross (MAGIC) lines. A total of 23 significant SNPs involved in stress resistance pathways were selected from a general linear model along with 31 SNPs reported from a FarmCPU model in previous studies. Of these 54 SNPs, 20 were selected in such a way to cover 13 stress-related genes. Kompetitive allele-specific PCR (KASP) assays were designed for the 20 selected SNPs and were subsequently used in validating the genotypes that were identified, six SNPs, viz, snpOS00912, snpOS00915, snpOS00922, snpOS00923, snpOS00927, and snpOS00929 as efficient in distinguishing the genotypes into BPH-resistant and susceptible clusters. Bph17 and Bph32 genes that are highly effective against the biotype 4 of the BPH have been validated by gene specific SNPs with favorable alleles in M201, M272, M344, RathuHeenati, and RathuHeenati accession. These identified genotypes could be useful as donors for transferring BPH resistance into popular varieties with marker-assisted selection using these diagnostic SNPs. The resistant lines and the significant SNPs unearthed from our study can be useful in developing BPH-resistant varieties after validating them in biparental populations with the potential usefulness of SNPs as causal markers

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Not AvailableAgronomic management of rice based cropping systems in sulfur deficient soilsiir

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Not AvailableRice (Oryza sativa L.) is grown under a range of hydrological regimes from the unflooded rainfed, through the rainfed and irrigated flooded, to the deep water and floating cultivation systems. It is the staple food for a large proportion of the world’s population. The geographic range of worldwide rice production is from the equator to the temperate areas of northern Japan and southern Australia and from sea level to attributes of more than 2500 m. The anaerobic soil environment created by flood irrigation of lowland rice creates a unique and challenging environment for the efficient management of soil and fertilizer nutrients. Supplying required essential nutrients in adequate rates, sources, application methods, and application times are important factors that influence the productivity and sustainability of rice.Not Availabl