Applications of UV-LIGA and grayscale lithography for display technologies

In the article MEMS technologies for display production and application presented. UV-LIGA and greyscale lithography based on SU-8 resist approaches were shown. Methods, technologies and structures of heterogeneous materials with soft magnetic properties, pros and cons are discussed. Unique specific parameters of soft magnetic composite material were achieved: magnetic induction of saturation – 2,1 Т, working frequency range – up to 1 MHz, permeability – up to 3000, total loss – 8 W/kg, Curie temperature – above 800 єС. Electroplating allows deposition of soft magnetic alloys on the conductive substrate. Metals like Fe, Ni, Co with additives like B, P were used to get the best soft magnetic properties. Special codeposition process was developed to allow insertion of soft magnetic composite powder filaments into soft magnetic matrix formed during. It allows developing magnetic micromotors for display production. Simulation of the hybrid step micromotors was carried out in Ansys Maxwell 19. It was demonstrated that it is possible to get 10 mN m tourqe under 25 μm rotor-stator air gap. Only presented microtechnologies can provide such accuracy of the mciromotors elements. As for greyscale photolithography, special grey mask were developed and it was demonstrated the possibility to produce controllable real 3D relief on the SU-8 photoresist. Thus, microtechnologies should be integrated into display technology to provide cost effective production and advanced properties of final products

Activated producing HOCL neutrophils revealed by flow cytometry and confocal microscopy with celestine blue B

Hypochlorous acid (HOCl) is produced with myeloperoxidase (MPO) in activated neutrophils. To assay MPO activity we chose a HOCl-selective dye i.e. celestine blue B (CB) that changes into pink glycol after oxidation. Our aim was to elaborate fluorescent methods of measuring HOCl production with activated neutrophils. Scanning CB fluorescence before and after its reaction with HOCl revealed activation and maximum emission, at 487 and 578 nm respectively, which are specific for oxidized product. Activated with Phorbol 12-myristate 13-acetate (PMA), the neutrophils were incubated with MPO inhibitor, 4-aminobenzoic acid hydrazide, which decreased fluorescence intensity (activation 487 nm, emission 578 nm) as compared with inhibitor-free samples. By confocal microscopy method we obtained images of CB- and DAPI-stained neutrophils. Neutrophil extracellular traps (NET) are clearly visible: DAP1 staining of multiple DNA bands co-localizes with fluorescence of oxidized CB. Flow cytometry showed that intensity of neutrophils activated by 50 nM PMA was 5 times higher (p < 0.05) than in PMA-free cells

A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES

Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships between the myeloperoxidase activity, native, and HOCl-modified ceruloplasmin levels in blood plasma samples of the patients with cardiovascular diseases.Specific antibodies against myeloperoxidase, ceruloplasmin, and HOCl-modified ceruloplasmin were obtained and specific enzyme-linked immunosorbent assays (ELISA) were developed. A combination of highly sensitive methods of myeloperoxidase assay i.e., solid-phase adsorption of antigens with subsequent testing of either their activity, or peroxidase-labeled antibody activity allowed elaborating the highly sensitive assays for ceruloplasmin and its HOCl-modified molecules, and for myeloperoxidase (concentration, peroxidase and halogenating activity). Positive correlation was proven between the myeloperoxidase concentration and activities. HOCl-modified ceruloplasmin content also correlated with myeloperoxidase activity.The HOCl-modified ceruloplasmin was first discovered in blood plasma samples from patients with cardiovascular diseases. In view of correlation between myeloperoxidase activity and HOCl-modified ceruloplasmin content in plasma, we suggest that HOCl production is aimed for suppression of myeloperoxidaseinhibitory function of ceruloplasmin

On the power of test for randomness on the base of lempel-ziv predictor

We propose a technique on the base of universal predictors for statistical test construction for randomness testing of binary sequences. The technique allows to find the asymptotic power of the test. We use the technique to construct the test on the base of the universal Lempel-Ziv predictor and theoretically find its power for the model of i.i.d. asymmetric Bernoulli trials under two-staged procedure of test construction. We perform comparison of the proposed test with Lempel-Ziv compression test from NIST SP800-22

Analysis of tests for randomness based on universal predictors: Bernoulli trials case

An approach to randomness testing for Bernoulli trials on the base of universal predictors is considered. We propose two strategies for using universal predictors and derive the power of statistical test constructed on the base of maximum-likelihood predictor for Bernoulli trials. The results are extended to CTW, SPM and Lempel-Ziv universal predictors. Comparison of test constructed on the base of Lempel-Ziv predictor with Lempel-Ziv compression test, proposed in NIST SP800-22, is performed

Analysis of tests for randomness based on universal predictors: Bernoulli trials case

An approach to randomness testing for Bernoulli trials on the base of universal predictors is considered. We propose two strategies for using universal predictors and derive the power of statistical test constructed on the base of maximum-likelihood predictor for Bernoulli trials. The results are extended to CTW, SPM and Lempel-Ziv universal predictors. Comparison of test constructed on the base of Lempel-Ziv predictor with Lempel-Ziv compression test, proposed in NIST SP800-22, is performed

On the power of test for randomness on the base of lempel-ziv predictor

We propose a technique on the base of universal predictors for statistical test construction for randomness testing of binary sequences. The technique allows to find the asymptotic power of the test. We use the technique to construct the test on the base of the universal Lempel-Ziv predictor and theoretically find its power for the model of i.i.d. asymmetric Bernoulli trials under two-staged procedure of test construction. We perform comparison of the proposed test with Lempel-Ziv compression test from NIST SP800-22

On Approach to Randomness Testing on the base of the Universal Predictors

The proposed approach allows to construct a statistical test for randomness using the known predictors that are universal for general alternative hypotheses. The test is shown to be consistent and to have a required significance level. An example of the test based on the Lempel-Ziv algorithm is given

Copper-Induced Oligomerization of Ceruloplasmin

Oligomerization of copper-containing ferroxidase of ceruloplasmin in the presence of protein-unbound copper in a solution has been investigated by small-angle X-ray scattering (SAXS). Ceruloplasmin is a monomer with a molecular weight of ~132 kDa. Copper chloride added in a concentration of 10 μM leads to dimerization of 60% of protein. According to the analysis of intersubunit contacts, dimerization can prevent the formation of protein‒protein complexes of ceruloplasmin with leukocyte proteins like myeloperoxidase and eosinophil peroxidase. The formation of these complexes is a part of organism’s protective response to inflammatory processes. In addition, dimeric ceruloplasmin probably loses its ability to bind and oxidize one of substrates p-phenylenediamine